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Rabbit Recombinant Monoclonal PDX1 antibody. Suitable for IHC-P, IP, WB, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 6 publications.

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Images

Western blot - Anti-PDX1 antibody [EPR22002] (AB219207), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDX1 antibody [EPR22002] (AB219207), expandable thumbnail
  • Western blot - Anti-PDX1 antibody [EPR22002] (AB219207), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-PDX1 antibody [EPR22002] (AB219207), expandable thumbnail
  • Immunoprecipitation - Anti-PDX1 antibody [EPR22002] (AB219207), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PIPWBICC/IF
Human
Tested
Expected
Tested
Expected
Mouse
Tested
Tested
Tested
Tested
Rat
Tested
Expected
Expected
Expected

Tested
Tested

Species
Mouse
Dilution info
1/500
Notes

Perform heat-mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 20 minutes.

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Species
Rat
Dilution info
1/500
Notes

Perform heat-mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 20 minutes.

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Species
Human
Dilution info
1/500
Notes

Perform heat-mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 20 minutes.

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Tested
Tested

Species
Mouse
Dilution info
1/30
Notes

-

Expected
Expected

Species
Rat, Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/1000
Notes

Human and rat species are recommended based on IHC result. This antibody is unsuitable to be used in western blot on human and rat samples.

Species
Human
Dilution info
1/1000
Notes

Human and rat species are recommended based on IHC result. This antibody is unsuitable to be used in western blot on human and rat samples.

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/1000
Notes

-

Expected
Expected

Species
Rat, Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Associated Products

Select an associated product type

9 products for Alternative Product

Target data

Function

Activates insulin, somatostatin, glucokinase, islet amyloid polypeptide and glucose transporter type 2 gene transcription. Particularly involved in glucose-dependent regulation of insulin gene transcription. As part of a PDX1:PBX1b:MEIS2b complex in pancreatic acinar cells is involved in the transcriptional activation of the ELA1 enhancer; the complex binds to the enhancer B element and cooperates with the transcription factor 1 complex (PTF1) bound to the enhancer A element. Binds preferentially the DNA motif 5'-[CT]TAAT[TG]-3'. During development, specifies the early pancreatic epithelium, permitting its proliferation, branching and subsequent differentiation. At adult stage, required for maintaining the hormone-producing phenotype of the beta-cell.

Alternative names

IPF1, STF1, PDX1, Pancreas/duodenum homeobox protein 1, PDX-1, Glucose-sensitive factor, Insulin promoter factor 1, Insulin upstream factor 1, Islet/duodenum homeobox-1, Somatostatin-transactivating factor 1, GSF, IPF-1, IUF-1, IDX-1, STF-1

Recommended products

Rabbit Recombinant Monoclonal PDX1 antibody. Suitable for IHC-P, IP, WB, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 6 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR22002
Purification technique
Affinity purification Protein A
Specificity

Human and rat species are recommended based on IHC result. This antibody is unsuitable to be used in western blot on human and rat samples.

Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Pancreatic duodenal homeobox 1 (PDX1) also known as insulin promoter factor 1 (IPF1) is an important transcription factor with a molecular weight of approximately 31 kDa. PDX1 plays an essential role in the development and function of the pancreas. This protein is mainly expressed in the pancreatic beta cells where it regulates gene expression linked to insulin production. Its expression also extends to the duodenum where it influences other developmental processes.

Biological function summary

PDX1 plays a critical role in pancreatic development beta-cell differentiation and insulin gene transcription. It functions as part of a protein complex that controls key aspects of beta-cell identity and function. The interactions within this complex allow PDX1 to effectively modulate pancreatic gene expression and maintain glucose homeostasis.

Pathways

PDX1 is deeply involved in the insulin secretion pathway and the regulation of glucose metabolism. It interacts with other transcription factors such as MAFA and NEUROD1 to carry out its role in these pathways. The presence of PDX1 in these pathways ensures the proper regulation of genes essential for insulin production and release connecting it to various metabolic processes.

Associated diseases and disorders

PDX1 associates mainly with diabetes mellitus and pancreatic agenesis. Mutations or functional impairments in PDX1 affect insulin production leading to diabetes. Furthermore in pancreatic agenesis where the pancreas fails to develop PDX1 malfunctions are often implicated. PDX1 interactions with proteins like MAFA and NEUROD1 during this process are critical; alterations in these relationships can exacerbate disease states linking PDX1 to both genetic and acquired forms of pancreatic dysfunction.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

  • Western blot - Anti-PDX1 antibody [EPR22002] (ab219207), expandable thumbnail

    Western blot - Anti-PDX1 antibody [EPR22002] (ab219207)

    Lane 1: 60 seconds

    Lane 2: 7 seconds

    All lanes: Anti-PDX1 antibody at 1/1000 dilution

    Lane 1: Caco-2 (Human colorectal adenocarcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 2: Beta-TC-6 (Mouse pancreas insulinoma beta cell) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 30 kDa

    Observed band size: 40 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDX1 antibody [EPR22002] (ab219207), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDX1 antibody [EPR22002] (ab219207)

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue labeling PDX1 with ab219207 at 1/500 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) ready to use. Nuclear and weak cytoplasmic staining in mouse pancreatic islet (PMID: 1847781, PMID: 22688334) is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) ready to use.

    Performed on a Leica Biosystems BOND® RX instrument.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Western blot - Anti-PDX1 antibody [EPR22002] (ab219207), expandable thumbnail

    Western blot - Anti-PDX1 antibody [EPR22002] (ab219207)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    The molecular mass observed is consistent with what has been described in the literature (PMID: 12488243)

    Negative control: NIH/3T3 (PMID: 25271154)

    All lanes: Western blot - Anti-PDX1 antibody [EPR22002] (ab219207) at 1/1000 dilution

    Lane 1: Beta-TC-6 (mouse pancreas insulinoma beta cell line) whole cell lysate at 10 µg

    Lane 2: NIH/3T3 (mouse embyro fibroblast cell line) whole cell lysate at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 30 kDa

    Observed band size: 46 kDa

    Exposure time: 92s

  • Immunocytochemistry/ Immunofluorescence - Anti-PDX1 antibody [EPR22002] (ab219207), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-PDX1 antibody [EPR22002] (ab219207)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Beta-TC-6 (mouse pancreas insulinoma beta cell line) and NIH/3T3 (mouse embyro fibroblast cell line) cells labeling PDX1 with ab219207 at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green).

    Confocal image showing nuclear staining in Beta-TC-6 cells.

    Negative control: NIH/3T3 (PMID: 25271154).

    The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889) (red) at 1/200 dilution.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution.

  • Immunoprecipitation - Anti-PDX1 antibody [EPR22002] (ab219207), expandable thumbnail

    Immunoprecipitation - Anti-PDX1 antibody [EPR22002] (ab219207)

    PFX1 was immunoprecipitated from 0.35 mg of Beta-TC-6 (mouse pancreas insulinoma beta cell line) whole cell lysate with ab219207 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab219207 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/5000 dilution.

    Lane 1: Beta-TC-6 whole cell lysate 10 μg (Input).
    Lane 2: ab219207 IP in Beta-TC-6 whole cell lysate.
    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab219207 in Beta-TC-6 whole cell lysate.

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: 3 seconds.

    The molecular mass observed is consistent with what has been described in the literature (PMID: 12488243).

    All lanes: Immunoprecipitation - Anti-PDX1 antibody [EPR22002] (ab219207)

    Predicted band size: 30 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDX1 antibody [EPR22002] (ab219207), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDX1 antibody [EPR22002] (ab219207)

    Immunohistochemical analysis of paraffin-embedded rat pancreas tissue labeling PDX1 with ab219207 at 1/500 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) ready to use. Nuclear and weak cytoplasmic staining in rat pancreatic islet (PMID: 1847781, PMID: 22688334) is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) ready to use.

    Performed on a Leica Biosystems BOND® RX instrument.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDX1 antibody [EPR22002] (ab219207), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDX1 antibody [EPR22002] (ab219207)

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue labeling PDX1 with ab219207 at 1/500 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) ready to use. Nuclear and weak cytoplasmic staining on human pancreatic islet and pancreatic acini (PMID: 1847781, PMID: 22688334) is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) ready to use.

    Performed on a Leica Biosystems BOND® RX instrument.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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