JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB84987

Anti-PDX1 antibody [OTI2A12]

Be the first to review this product! Submit a review

|

(6 Publications )

Mouse Monoclonal PDX1 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 6 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human PDX1.
6 Images
Immunocytochemistry/ Immunofluorescence - Anti-PDX1 antibody [OTI2A12] (AB84987)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-PDX1 antibody [OTI2A12] (AB84987)

ab84987, staining PDX1 by Immunofluoresence in HeLa cells transiently transfected with a vector expressing PDX1.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDX1 antibody [OTI2A12] (AB84987)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDX1 antibody [OTI2A12] (AB84987)

ab84987, staining PDX1 in paraffin embedded human pancreas carcinoma tissue by Immunohistochemistry.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDX1 antibody [OTI2A12] (AB84987)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDX1 antibody [OTI2A12] (AB84987)

ab84987, staining PDX1 in paraffin embedded human pancreas by Immunohistochemistry.

Western blot - Anti-PDX1 antibody [OTI2A12] (AB84987)
  • WB

Unknown

Western blot - Anti-PDX1 antibody [OTI2A12] (AB84987)

HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC200033)

All lanes:

Western blot - Anti-PDX1 antibody [OTI2A12] (ab84987)

Lane 1:

lysate from HEK293T cells transfected with an empty expression vector at 5 µg

Lane 2:

lysate from HEK293T cells transfected with a human PDX1 expression vector at 5 µg

Predicted band size: 30 kDa

Observed band size: 31 kDa

false

Western blot - Anti-PDX1 antibody [OTI2A12] (AB84987)
  • WB

Unknown

Western blot - Anti-PDX1 antibody [OTI2A12] (AB84987)

HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC200033)

All lanes:

Western blot - Anti-PDX1 antibody [OTI2A12] (ab84987) at 1/20000 dilution

All lanes:

lysate from HEK293T cells transfected with human PDX1 expression vector

Predicted band size: 30 kDa

false

Western blot - Anti-PDX1 antibody [OTI2A12] (AB84987)
  • WB

CiteAb

Western blot - Anti-PDX1 antibody [OTI2A12] (AB84987)

PDX1 western blot using anti-PDX1 antibody [OTI2A12] ab84987. Publication image and figure legend from Jafarian, A., Taghikani, M., et al., 2015, PLoS One, PubMed 26047014.

ab84987 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab84987 please see the product overview.

Detection of pancreatic endocrine proteins levels by western blot in IPCs.Western blot analysis detected expressions of insulin (5KD), glucagon (46KD), PDX1 (31KD) and Ngn3 (23KD) in differentiated IPCs (1) MSCsmiR-375 and (2) MSCsmiR-375+anti-miR-9 (3) MSCscontrol.

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

OTI2A12

Isotype

IgG1

Carrier free

No

Reacts with

Human

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human PDX1.

P52945

style
left-column

Alternative Products

Primary Antibodies

AB196464

Alexa Fluor® 488 Anti-PDX1 antibody [EPR3358(2)]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-PDX1 antibody [EPR3358(2)] (AB196464)

  • 0
  • 0
Primary Antibodies

AB219207

Anti-PDX1 antibody [EPR22002]

BOND RX™ Validated|RabMAb|Recombinant|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDX1 antibody [EPR22002] (AB219207)

  • 0
  • 0
Primary Antibodies

AB234633

Anti-PDX1 antibody [EPR22002] - BSA and Azide free

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDX1 antibody [EPR22002] - BSA and Azide free (AB234633)

  • 0
  • 0
Primary Antibodies

AB134150

Anti-PDX1 antibody [EPR3358(2)]

20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDX1 antibody [EPR3358(2)] (AB134150)

  • 5
  • 1
Primary Antibodies

AB240084

Anti-PDX1 antibody [EPR3358(2)] - BSA and Azide free

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDX1 antibody [EPR3358(2)] - BSA and Azide free (AB240084)

  • 0
  • 0
Primary Antibodies

AB196195

Alexa Fluor® 647 Anti-PDX1 antibody [EPR3358(2)]

Recombinant|RabMAb

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-PDX1 antibody [EPR3358(2)] (AB196195)

  • 0
  • 0

Complementary Products

Assay Kits

AB102513

Adipogenesis Detection Assay Kit (Colorimetric/Fluorometric)

Functional Studies - Adipogenesis Detection Assay Kit (Colorimetric/Fluorometric) (AB102513)

  • 0
  • 0
Primary Antibodies

AB92517

Anti-Glucagon antibody [EP3070]

RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucagon antibody [EP3070] (AB92517)

  • 5
  • 8
Primary Antibodies

AB282459

Anti-Insulin antibody [RM1019]

RabMAb|Recombinant|Lab Essentials|20ul selling size|Trial Size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Insulin antibody [RM1019] (AB282459)

  • 0
  • 0
Primary Antibodies

AB221549

Anti-nkx6.1 antibody [EPR20405]

RabMAb|Recombinant|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-nkx6.1 antibody [EPR20405] (AB221549)

  • 5
  • 2
Primary Antibodies

AB16667

Anti-Ki67 antibody [SP6]

BOND RX™ Validated|KO Validated|RabMAb|Advanced Validation|Recombinant|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [SP6] (AB16667)

  • 5
  • 133
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/20000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }
style
left-column

Product details

The clone number has been updated from 2A12 to OTI2A12, both clone numbers name the same clone.
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Purified from TCS
Storage buffer
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Pancreatic duodenal homeobox 1 (PDX1) also known as insulin promoter factor 1 (IPF1) is an important transcription factor with a molecular weight of approximately 31 kDa. PDX1 plays an essential role in the development and function of the pancreas. This protein is mainly expressed in the pancreatic beta cells where it regulates gene expression linked to insulin production. Its expression also extends to the duodenum where it influences other developmental processes.
Biological function summary

PDX1 plays a critical role in pancreatic development beta-cell differentiation and insulin gene transcription. It functions as part of a protein complex that controls key aspects of beta-cell identity and function. The interactions within this complex allow PDX1 to effectively modulate pancreatic gene expression and maintain glucose homeostasis.

Pathways

PDX1 is deeply involved in the insulin secretion pathway and the regulation of glucose metabolism. It interacts with other transcription factors such as MAFA and NEUROD1 to carry out its role in these pathways. The presence of PDX1 in these pathways ensures the proper regulation of genes essential for insulin production and release connecting it to various metabolic processes.

PDX1 associates mainly with diabetes mellitus and pancreatic agenesis. Mutations or functional impairments in PDX1 affect insulin production leading to diabetes. Furthermore in pancreatic agenesis where the pancreas fails to develop PDX1 malfunctions are often implicated. PDX1 interactions with proteins like MAFA and NEUROD1 during this process are critical; alterations in these relationships can exacerbate disease states linking PDX1 to both genetic and acquired forms of pancreatic dysfunction.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Activates insulin, somatostatin, glucokinase, islet amyloid polypeptide and glucose transporter type 2 gene transcription. Particularly involved in glucose-dependent regulation of insulin gene transcription. As part of a PDX1 : PBX1b : MEIS2b complex in pancreatic acinar cells is involved in the transcriptional activation of the ELA1 enhancer; the complex binds to the enhancer B element and cooperates with the transcription factor 1 complex (PTF1) bound to the enhancer A element. Binds preferentially the DNA motif 5'-[CT]TAAT[TG]-3'. During development, specifies the early pancreatic epithelium, permitting its proliferation, branching and subsequent differentiation. At adult stage, required for maintaining the hormone-producing phenotype of the beta-cell.
See full target information PDX1

Alternative Names

IPF1, STF1, PDX1, Pancreas/duodenum homeobox protein 1, PDX-1, Glucose-sensitive factor, Insulin promoter factor 1, Insulin upstream factor 1, Islet/duodenum homeobox-1, Somatostatin-transactivating factor 1, GSF, IPF-1, IUF-1, IDX-1, STF-1
style
left-column

Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Biochemistry and biophysics reports 30:101273 PubMed35592615

2022

Micro-RNA-124-5p promotes insulin producing cell differentiation through regulating transcriptional factor NKX6.1.

Applications

Unspecified application

Species

Unspecified reactive species

Xianjun Zhang,Shunzi Shao,Xijiang Zhao,Meng Zhang,Jingbo Wang

Life sciences 274:119338 PubMed33716064

2021

Treatment of diabetic mice by microfluidic system-assisted transplantation of stem cells-derived insulin-producing cells transduced with miRNA.

Applications

Unspecified application

Species

Unspecified reactive species

Adele Soltani,Masoud Soleimani,Mohammad Adel Ghiass,Seyed Ehsan Enderami,Shahram Rabbani,Arefeh Jafarian,Abdolamir Allameh

Journal of tissue engineering 8:2041731417738145 PubMed29118967

2017

Cold-perfusion decellularization of whole-organ porcine pancreas supports human fetal pancreatic cell attachment and expression of endocrine and exocrine markers.

Applications

Unspecified application

Species

Unspecified reactive species

Erik Elebring,Vijay K Kuna,Niclas Kvarnström,Suchitra Sumitran-Holgersson

Cell journal 17:231-42 PubMed26199902

2015

Differentiation of Human Mesenchymal Stem Cells into Insulin Producing Cells by Using A Lentiviral Vector Carrying PDX1.

Applications

WB

Species

Unspecified reactive species

Amir Allahverdi,Saied Abroun,Arefeh Jafarian,Masoud Soleimani,Mohammad Taghikhani,Fatemeh Eskandari

PloS one 10:e0128650 PubMed26047014

2015

The Generation of Insulin Producing Cells from Human Mesenchymal Stem Cells by MiR-375 and Anti-MiR-9.

Applications

IF

Species

Unspecified reactive species

Arefeh Jafarian,Mohammad Taghikani,Saeid Abroun,Amir Allahverdi,Maryam Lamei,Niknam Lakpour,Masoud Soleimani

Cancer gene therapy 20:683-9 PubMed24287722

2013

Assessment of intravenous pbi-shRNA PDX1 nanoparticle (OFHIRNA-PDX1) in yucatan swine.

Applications

Unspecified application

Species

Unspecified reactive species

C M Jay,C Ruoff,P Kumar,H Maass,B Spanhel,M Miller,A Arrington,N Montalvo,V Gresham,D D Rao,C Evans,Z Wang,F C Brunicardi,S-H Liu,G Zhou,N Senzer,J Nemunaitis,L King,B Weeks,F J Clubb,T W Fossum,P B Maples
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com