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Mouse Monoclonal PDX1 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human PDX1.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDX1 antibody [OTI2A12] (AB84987), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDX1 antibody [OTI2A12] (AB84987), expandable thumbnail
  • Western blot - Anti-PDX1 antibody [OTI2A12] (AB84987), expandable thumbnail
  • Western blot - Anti-PDX1 antibody [OTI2A12] (AB84987), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-PDX1 antibody [OTI2A12] (AB84987), expandable thumbnail

Publications

Key facts

Isotype
IgG1
Host species
Mouse
Storage buffer

pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • Recombinant Full Length Protein corresponding to Human PDX1. Database link P52945

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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-PICC/IF
Human
Tested
Tested
Tested

Tested
Tested

Species
Human
Dilution info
1/20000
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Associated Products

Select an associated product type

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Target data

Function

Activates insulin, somatostatin, glucokinase, islet amyloid polypeptide and glucose transporter type 2 gene transcription. Particularly involved in glucose-dependent regulation of insulin gene transcription. As part of a PDX1:PBX1b:MEIS2b complex in pancreatic acinar cells is involved in the transcriptional activation of the ELA1 enhancer; the complex binds to the enhancer B element and cooperates with the transcription factor 1 complex (PTF1) bound to the enhancer A element. Binds preferentially the DNA motif 5'-[CT]TAAT[TG]-3'. During development, specifies the early pancreatic epithelium, permitting its proliferation, branching and subsequent differentiation. At adult stage, required for maintaining the hormone-producing phenotype of the beta-cell.

Alternative names

IPF1, STF1, PDX1, Pancreas/duodenum homeobox protein 1, PDX-1, Glucose-sensitive factor, Insulin promoter factor 1, Insulin upstream factor 1, Islet/duodenum homeobox-1, Somatostatin-transactivating factor 1, GSF, IPF-1, IUF-1, IDX-1, STF-1

Recommended products

Mouse Monoclonal PDX1 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human PDX1.

Key facts

Isotype
IgG1
Form
Liquid
Clonality
Monoclonal
Immunogen
  • Recombinant Full Length Protein corresponding to Human PDX1. Database link P52945
Clone number
OTI2A12
Purification technique
Affinity purification Immunogen
Concentration
Loading...
Purification notes

Purified from TCS

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

The clone number has been updated from 2A12 to OTI2A12, both clone numbers name the same clone.

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Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Pancreatic duodenal homeobox 1 (PDX1) also known as insulin promoter factor 1 (IPF1) is an important transcription factor with a molecular weight of approximately 31 kDa. PDX1 plays an essential role in the development and function of the pancreas. This protein is mainly expressed in the pancreatic beta cells where it regulates gene expression linked to insulin production. Its expression also extends to the duodenum where it influences other developmental processes.

Biological function summary

PDX1 plays a critical role in pancreatic development beta-cell differentiation and insulin gene transcription. It functions as part of a protein complex that controls key aspects of beta-cell identity and function. The interactions within this complex allow PDX1 to effectively modulate pancreatic gene expression and maintain glucose homeostasis.

Pathways

PDX1 is deeply involved in the insulin secretion pathway and the regulation of glucose metabolism. It interacts with other transcription factors such as MAFA and NEUROD1 to carry out its role in these pathways. The presence of PDX1 in these pathways ensures the proper regulation of genes essential for insulin production and release connecting it to various metabolic processes.

Associated diseases and disorders

PDX1 associates mainly with diabetes mellitus and pancreatic agenesis. Mutations or functional impairments in PDX1 affect insulin production leading to diabetes. Furthermore in pancreatic agenesis where the pancreas fails to develop PDX1 malfunctions are often implicated. PDX1 interactions with proteins like MAFA and NEUROD1 during this process are critical; alterations in these relationships can exacerbate disease states linking PDX1 to both genetic and acquired forms of pancreatic dysfunction.

Product promise

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6 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDX1 antibody [OTI2A12] (ab84987), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDX1 antibody [OTI2A12] (ab84987)

    ab84987, staining PDX1 in paraffin embedded human pancreas carcinoma tissue by Immunohistochemistry.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDX1 antibody [OTI2A12] (ab84987), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDX1 antibody [OTI2A12] (ab84987)

    ab84987, staining PDX1 in paraffin embedded human pancreas by Immunohistochemistry.

  • Western blot - Anti-PDX1 antibody [OTI2A12] (ab84987), expandable thumbnail

    Western blot - Anti-PDX1 antibody [OTI2A12] (ab84987)

    HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC200033)

    All lanes: Western blot - Anti-PDX1 antibody [OTI2A12] (ab84987) at 1/20000 dilution

    All lanes: lysate from HEK293T cells transfected with human PDX1 expression vector

    Predicted band size: 30 kDa

  • Western blot - Anti-PDX1 antibody [OTI2A12] (ab84987), expandable thumbnail

    Western blot - Anti-PDX1 antibody [OTI2A12] (ab84987)

    HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC200033)

    All lanes: Western blot - Anti-PDX1 antibody [OTI2A12] (ab84987)

    Lane 1: lysate from HEK293T cells transfected with an empty expression vector at 5 µg

    Lane 2: lysate from HEK293T cells transfected with a human PDX1 expression vector at 5 µg

    Predicted band size: 30 kDa

    Observed band size: 31 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-PDX1 antibody [OTI2A12] (ab84987), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-PDX1 antibody [OTI2A12] (ab84987)

    ab84987, staining PDX1 by Immunofluoresence in HeLa cells transiently transfected with a vector expressing PDX1.

  • Western blot - Anti-PDX1 antibody [OTI2A12] (ab84987), expandable thumbnail

    Image collected and cropped by CiteAb under a CC-BY license from the publication

    Western blot - Anti-PDX1 antibody [OTI2A12] (ab84987)

    PDX1 western blot using anti-PDX1 antibody [OTI2A12] ab84987. Publication image and figure legend from Jafarian, A., Taghikani, M., et al., 2015, PLoS One, PubMed 26047014.


    ab84987 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab84987 please see the product overview.

    Detection of pancreatic endocrine proteins levels by western blot in IPCs.Western blot analysis detected expressions of insulin (5KD), glucagon (46KD), PDX1 (31KD) and Ngn3 (23KD) in differentiated IPCs (1) MSCsmiR-375 and (2) MSCsmiR-375+anti-miR-9 (3) MSCscontrol.

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