Rabbit Polyclonal PDXK.1 antibody. Suitable for WB, IHC-P and reacts with Rat, Recombinant full length protein - Rat, Mouse, Human samples. Immunogen corresponding to Recombinant Fragment Protein within Rat Pdxk aa 1-300.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.011% Proclin 300
Constituents: 55.77% Glycerol (glycerin, glycerine), 44.219% PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Rat Pdxk aa 1-300. The exact immunogen used to generate this antibody is proprietary information. Database link O35331
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Tested | Expected |
| Mouse | Tested | Expected |
| Rat | Tested | Tested |
| Recombinant full length protein - Rat | Tested | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 0.50000-2.00000 µg/mL | Notes - |
Species Recombinant full length protein - Rat | Dilution info 0.50000-2.00000 µg/mL | Notes - |
Species Mouse | Dilution info 0.50000-2.00000 µg/mL | Notes - |
Species Human | Dilution info 0.50000-2.00000 µg/mL | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 5.00000-20.00000 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
Catalyzes the phosphorylation of the dietary vitamin B6 vitamers pyridoxal (PL), pyridoxine (PN) and pyridoxamine (PM) to form pyridoxal 5'-phosphate (PLP), pyridoxine 5'-phosphate (PNP) and pyridoxamine 5'-phosphate (PMP), respectively (Probable) (PubMed:10987144, PubMed:17766369, PubMed:19351586, PubMed:31187503, PubMed:9099727). PLP is the active form of vitamin B6, and acts as a cofactor for over 140 different enzymatic reactions.
Alternative names
C21orf124, C21orf97, PKH, PNK, PRED79, PDXK, Pyridoxal kinase, Pyridoxine kinase
Recommended products
Rabbit Polyclonal PDXK.1 antibody. Suitable for WB, IHC-P and reacts with Rat, Recombinant full length protein - Rat, Mouse, Human samples. Immunogen corresponding to Recombinant Fragment Protein within Rat Pdxk aa 1-300.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.011% Proclin 300
Constituents: 55.77% Glycerol (glycerin, glycerine), 44.219% PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Rat Pdxk aa 1-300. The exact immunogen used to generate this antibody is proprietary information. Database link O35331
- Purification technique
- Affinity purification Protein A
- Concentration
- Purification notes
Antigen-specific affinity chromatography followed by Protein A affinity chromatography.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
PDXK.1 also known as pyridoxal kinase is an important enzyme that facilitates the phosphorylation of vitamin B6 to pyridoxal-5'-phosphate (PLP). Its molecular mass is approximately 35 kDa. You can find it expressed mainly in the liver kidney and brain tissues. PDXK.1 plays a role in converting vitamin B6 which is necessary for various enzymatic activities throughout the body.
- Biological function summary
PDXK.1 functions within cellular environments by catalyzing the formation of PLP an active form of vitamin B6. PLP acts as a coenzyme involved in numerous reactions such as amino acid metabolism neurotransmitter synthesis and modulation of hormone activity. PDXK.1 does not work in isolation but instead interacts with other proteins in these processes though it is not a part of a larger complex.
- Pathways
PDXK.1 is important for the vitamin B6 metabolism pathway. This pathway integrates with networks involving amino acid catabolism and neurotransmitter pathways essential for metabolic functions and nervous system regulation. An association exists between PDXK.1 and proteins like cysteine desulfurase which also rely on PLP for their enzymatic activities highlighting its involvement in broader biochemical systems.
- Associated diseases and disorders
PDXK.1 has been linked with metabolic disorders and neurological conditions. A deficiency or dysfunction in PDXK.1 activity can contribute to conditions such as pyridoxine-dependent epilepsy and some forms of anemia. In these cases disruptions in the metabolism of vitamin B6 due to inadequate levels of PLP may directly affect proteins like glutamate decarboxylase which require PLP for proper function.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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7 product images
Western blot - Anti-PDXK.1 antibody (ab232779)
All lanes: Western blot - Anti-PDXK.1 antibody (ab232779) at 2 µg/mL
Lane 1: Rat liver tissue lysate
Lane 2: Rat lung tissue lysate
Lane 3: Mouse liver tissue lysate
Lane 4: HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate
SecondaryAll lanes: HRP-linked Guinea pig anti-Rabbit at 1/2000 dilution
Predicted band size: 35 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDXK.1 antibody (ab232779)
Formalin-fixed, paraffin-embedded rat pancreas tissue stained for PDXK.1 with ab232779 at 10 μg/ml in immunohistochemical analysis. DAB staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDXK.1 antibody (ab232779)
Formalin-fixed, paraffin-embedded rat cerebrum tissue stained for PDXK.1 with ab232779 at 10 μg/ml in immunohistochemical analysis. DAB staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDXK.1 antibody (ab232779)
Formalin-fixed, paraffin-embedded rat adrenal gland tissue stained for PDXK.1 with ab232779 at 10 μg/ml in immunohistochemical analysis. DAB staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDXK.1 antibody (ab232779)
Formalin-fixed, paraffin-embedded rat spinal cord tissue stained for PDXK.1 with ab232779 at 10 μg/ml in immunohistochemical analysis. DAB staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDXK.1 antibody (ab232779)
Formalin-fixed, paraffin-embedded rat liver tissue stained for PDXK.1 with ab232779 at 10 μg/ml in immunohistochemical analysis. DAB staining.
Western blot - Anti-PDXK.1 antibody (ab232779)
All lanes: Western blot - Anti-PDXK.1 antibody (ab232779) at 2 µg/mL
All lanes: Recombinant rat PDXK.1 protein
Predicted band size: 35 kDa
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