AB232779

Anti-PDXK antibody

Name: Anti-PDXK antibody (ab232779) | Abcam
Brand: Abcam Limited
SKU: AB232779
Price: 510 USD
Availability: InStock

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(1 Publication)

Rabbit Polyclonal PDXK.1 antibody. Suitable for WB, IHC-P and reacts with Rat, Recombinant full length protein - Rat, Mouse, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Rat Pdxk aa 1-300.

View Alternative Names

C21orf124, C21orf97, PKH, PNK, PRED79, PDXK, Pyridoxal kinase, Pyridoxine kinase

7 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDXK antibody (AB232779)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDXK antibody (AB232779)

Formalin-fixed, paraffin-embedded rat adrenal gland tissue stained for PDXK.1 with ab232779 at 10 μg/ml in immunohistochemical analysis. DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDXK antibody (AB232779)

Formalin-fixed, paraffin-embedded rat spinal cord tissue stained for PDXK.1 with ab232779 at 10 μg/ml in immunohistochemical analysis. DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDXK antibody (AB232779)

Formalin-fixed, paraffin-embedded rat liver tissue stained for PDXK.1 with ab232779 at 10 μg/ml in immunohistochemical analysis. DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDXK antibody (AB232779)

Formalin-fixed, paraffin-embedded rat pancreas tissue stained for PDXK.1 with ab232779 at 10 μg/ml in immunohistochemical analysis. DAB staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDXK.1 antibody (AB232779)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDXK.1 antibody (AB232779)

Formalin-fixed, paraffin-embedded rat cerebrum tissue stained for PDXK.1 with ab232779 at 10 μg/ml in immunohistochemical analysis. DAB staining.

Supplier Data

Western blot - Anti-PDXK antibody (AB232779)

All lanes:

Western blot - Anti-PDXK antibody (ab232779) at 2 µg/mL

Lane 1:

Rat liver tissue lysate

Lane 2:

Rat lung tissue lysate

Lane 3:

Mouse liver tissue lysate

Lane 4:

HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate

Secondary

All lanes:

HRP-linked Guinea pig anti-Rabbit at 1/2000 dilution

Predicted band size: 35 kDa

false

Supplier Data

Western blot - Anti-PDXK antibody (AB232779)

All lanes:

Western blot - Anti-PDXK antibody (ab232779) at 2 µg/mL

All lanes:

Recombinant rat PDXK protein

Predicted band size: 35 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Rat Pdxk aa 1-300. The exact immunogen used to generate this antibody is proprietary information.

O35331

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-2 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-2 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-2 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5-20 µg/mL", "IHCP-species-notes": "" }, "Recombinant full length protein - Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-2 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Purification notes

Antigen-specific affinity chromatography followed by Protein A affinity chromatography.

Storage buffer

pH: 7.4 Preservative: 0.011% Proclin 300 Constituents: PBS, 55.77% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PDXK also known as pyridoxal kinase is an important enzyme that facilitates the phosphorylation of vitamin B6 to pyridoxal-5'-phosphate (PLP). Its molecular mass is approximately 35 kDa. You can find it expressed mainly in the liver kidney and brain tissues. PDXK plays a role in converting vitamin B6 which is necessary for various enzymatic activities throughout the body.

Biological function summary

PDXK functions within cellular environments by catalyzing the formation of PLP an active form of vitamin B6. PLP acts as a coenzyme involved in numerous reactions such as amino acid metabolism neurotransmitter synthesis and modulation of hormone activity. PDXK does not work in isolation but instead interacts with other proteins in these processes though it is not a part of a larger complex.

Pathways

PDXK is important for the vitamin B6 metabolism pathway. This pathway integrates with networks involving amino acid catabolism and neurotransmitter pathways essential for metabolic functions and nervous system regulation. An association exists between PDXK and proteins like cysteine desulfurase which also rely on PLP for their enzymatic activities highlighting its involvement in broader biochemical systems.

PDXK has been linked with metabolic disorders and neurological conditions. A deficiency or dysfunction in PDXK activity can contribute to conditions such as pyridoxine-dependent epilepsy and some forms of anemia. In these cases disruptions in the metabolism of vitamin B6 due to inadequate levels of PLP may directly affect proteins like glutamate decarboxylase which require PLP for proper function.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes the phosphorylation of the dietary vitamin B6 vitamers pyridoxal (PL), pyridoxine (PN) and pyridoxamine (PM) to form pyridoxal 5'-phosphate (PLP), pyridoxine 5'-phosphate (PNP) and pyridoxamine 5'-phosphate (PMP), respectively (Probable) (PubMed : 10987144, PubMed : 17766369, PubMed : 19351586, PubMed : 31187503, PubMed : 9099727). PLP is the active form of vitamin B6, and acts as a cofactor for over 140 different enzymatic reactions.

See full target information PDXK

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

American journal of translational research 14:4310-4320 PubMed35836868

2022

Follistatin-like 1 ameliorates severe acute pancreatitis associated lung injury via inhibiting the activation of NLRP3 inflammasome and NF-κB pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Liming Wang,Na Wang,Guifang Shi,Shuqing Sun

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PDXK antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDXK antibody (AB232779)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDXK antibody (AB232779)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDXK antibody (AB232779)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDXK antibody (AB232779)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDXK.1 antibody (AB232779)

Western blot - Anti-PDXK antibody (AB232779)

Western blot - Anti-PDXK antibody (AB232779)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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