AB251802

Anti-PDXK antibody

Name: Anti-PDXK antibody (ab251802) | Abcam
Brand: Abcam Limited
SKU: AB251802

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(1 Publication)

Rabbit Polyclonal PDXK.1 antibody. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human PDXK aa 100-250.

View Alternative Names

C21orf124, C21orf97, PKH, PNK, PRED79, PDXK, Pyridoxal kinase, Pyridoxine kinase

6 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDXK antibody (AB251802)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDXK antibody (AB251802)

Paraffin-embedded human testis tissue stained for PDXK using ab251802 at 1/1000 dilution in immunohistochemical analysis.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDXK antibody (AB251802)

Paraffin-embedded human skeletal muscle tissue stained for PDXK using ab251802 at 1/1000 dilution in immunohistochemical analysis.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDXK antibody (AB251802)

Paraffin-embedded human cerebral cortex tissue stained for PDXK using ab251802 at 1/1000 dilution in immunohistochemical analysis.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDXK antibody (AB251802)

Paraffin-embedded human kidney tissue stained for PDXK using ab251802 at 1/1000 dilution in immunohistochemical analysis.

Unknown

Western blot - Anti-PDXK antibody (AB251802)

Western Blot analysis of ab251802 (A) shows a similar pattern to an independent antibody (B).

All lanes:

Western blot - Anti-PDXK antibody (ab251802) at 0.4 µg/mL

Lane 1:

RT4 (Human urinary bladder cancer cell line) whole cell lysate

Lane 2:

U-251 MG (formally U-373 MG) (human brain glioma cell line) whole cell lysate

Lane 3:

Human plasma

Lane 4:

Human liver tissue lysate

Lane 5:

Human tonsil tissue lysate

Predicted band size: 35 kDa

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Unknown

Western blot - Anti-PDXK antibody (AB251802)

All lanes:

Western blot - Anti-PDXK antibody (ab251802) at 0.4 µg/mL

Lane 1:

NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate

Lane 2:

NBT-II (Rat Wistar bladder tumour cells) cell lysate

Predicted band size: 35 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human PDXK aa 100-250. The exact immunogen used to generate this antibody is proprietary information.

O00764

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000 - 1/2500", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PDXK also known as pyridoxal kinase is an important enzyme that facilitates the phosphorylation of vitamin B6 to pyridoxal-5'-phosphate (PLP). Its molecular mass is approximately 35 kDa. You can find it expressed mainly in the liver kidney and brain tissues. PDXK plays a role in converting vitamin B6 which is necessary for various enzymatic activities throughout the body.

Biological function summary

PDXK functions within cellular environments by catalyzing the formation of PLP an active form of vitamin B6. PLP acts as a coenzyme involved in numerous reactions such as amino acid metabolism neurotransmitter synthesis and modulation of hormone activity. PDXK does not work in isolation but instead interacts with other proteins in these processes though it is not a part of a larger complex.

Pathways

PDXK is important for the vitamin B6 metabolism pathway. This pathway integrates with networks involving amino acid catabolism and neurotransmitter pathways essential for metabolic functions and nervous system regulation. An association exists between PDXK and proteins like cysteine desulfurase which also rely on PLP for their enzymatic activities highlighting its involvement in broader biochemical systems.

PDXK has been linked with metabolic disorders and neurological conditions. A deficiency or dysfunction in PDXK activity can contribute to conditions such as pyridoxine-dependent epilepsy and some forms of anemia. In these cases disruptions in the metabolism of vitamin B6 due to inadequate levels of PLP may directly affect proteins like glutamate decarboxylase which require PLP for proper function.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes the phosphorylation of the dietary vitamin B6 vitamers pyridoxal (PL), pyridoxine (PN) and pyridoxamine (PM) to form pyridoxal 5'-phosphate (PLP), pyridoxine 5'-phosphate (PNP) and pyridoxamine 5'-phosphate (PMP), respectively (Probable) (PubMed : 10987144, PubMed : 17766369, PubMed : 19351586, PubMed : 31187503, PubMed : 9099727). PLP is the active form of vitamin B6, and acts as a cofactor for over 140 different enzymatic reactions.

See full target information PDXK

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cancer cell international 20:354 PubMed32760221

2020

Enhanced expression of microtubule-associated protein 7 functioned as a contributor to cervical cancer cell migration and is predictive of adverse prognosis.

Applications

Unspecified application

Species

Unspecified reactive species

Ning Tang,Dan Lyu,Jian-Fang Chang,Zhi-Tao Liu,Yan Zhang,Hai-Ping Liu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PDXK antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDXK antibody (AB251802)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDXK antibody (AB251802)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDXK antibody (AB251802)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDXK antibody (AB251802)

Western blot - Anti-PDXK antibody (AB251802)

Western blot - Anti-PDXK antibody (AB251802)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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