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AB244888

Anti-PDZ Binding Kinase antibody [EPR2520-35] - BSA and Azide free (Detector)

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Rabbit Recombinant Monoclonal PBK/SPK antibody. Carrier free. Suitable for sELISA and reacts with Human samples.

View Alternative Names

TOPK, PBK, Lymphokine-activated killer T-cell-originated protein kinase, Cancer/testis antigen 84, MAPKK-like protein kinase, Nori-3, PDZ-binding kinase, Spermatogenesis-related protein kinase, T-LAK cell-originated protein kinase, CT84, SPK

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Sandwich ELISA - Anti-PDZ Binding Kinase antibody [EPR2520-35] - BSA and Azide free (Detector) (AB244888)
  • sELISA

Unknown

Sandwich ELISA - Anti-PDZ Binding Kinase antibody [EPR2520-35] - BSA and Azide free (Detector) (AB244888)

Representative standard curve from corresponding SimpleStep ELISA® Kit (ab184866).

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR2520-35

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }
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You may be interested in:

AB184866

Human PBK ELISA Kit

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We recommend this product because it’s often used in the same experiment or related research.

We advise that you always check the datasheet to ensure it fits your experiments, or contact ourtechnical teamfor help.

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Product details

ab244888 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human PBK. The recommended pair for sandwich ELISA is:
Capture: ab244628, Human PBK Capture Antibody (unconjugated)
Detector: ab244888, Human PBK Detector Antibody (unconjugated)
The reference range value is 300 - 5000 pg/ml.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Constituents: 100% PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PDZ Binding Kinase (PBK) also known as TOPK is a serine/threonine kinase with a molecular mass of approximately 36 kDa. It performs phosphorylation on certain proteins influencing their function localization or interaction with other proteins. PBK locates mainly in the testes placenta and various tumors but also expresses in smaller amounts in normal tissues such as the liver and brain. The kinase is cell cycle-dependent with activity peaking during mitosis where it plays a role by interacting with the mitotic spindle apparatus.
Biological function summary

PBK acts as part of cellular proliferation and differentiation processes. It can form complexes with other proteins enhancing its function and stability. The kinase significantly contributes to cell growth mainly in rapidly dividing cells. PBK influences processes like spermatogenesis in testes and placental development. PBK stabilizes certain cyclins and links with proteins such as p38 MAP kinase showing a role in stress response pathways.

Pathways

PBK integrates into the mitogen-activated protein kinase (MAPK) pathway and interacts with the tumor progression pathway. These pathways are critical for transmitting signals from the surface to the nucleus affecting cell fate decisions. PBK connects with proteins like JNK and extracellular signal-regulated kinase (ERK) displaying its importance in transmitting stress and growth signals. These interactions place PBK as a node linking growth factors and extracellular signals to cellular responses.

PBK is significantly associated with cancer development and progression such as in prostate cancer and glioma. In these conditions PBK often exhibits overexpression facilitating enhanced proliferation and survival of tumor cells. The connection to proteins like Cdc2/cyclin B1 promotes unchecked cell cycle progression often found in neoplasms. PBK's role in these disorders makes it a potential biomarker or therapeutic target in oncology.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Phosphorylates MAP kinase p38. Seems to be active only in mitosis. May also play a role in the activation of lymphoid cells. When phosphorylated, forms a complex with TP53, leading to TP53 destabilization and attenuation of G2/M checkpoint during doxorubicin-induced DNA damage.
See full target information PBK
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com