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AB110059

Anti-PDZ-RhoGEF antibody

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(4 Publications)

Rabbit Polyclonal PDZ-RhoGEF antibody. Suitable for WB and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human ARHGEF11.

View Alternative Names

KIAA0380, ARHGEF11, Rho guanine nucleotide exchange factor 11, PDZ-RhoGEF

1 Images
Western blot - Anti-PDZ-RhoGEF antibody (AB110059)
  • WB

Unknown

Western blot - Anti-PDZ-RhoGEF antibody (AB110059)

All lanes:

Western blot - Anti-PDZ-RhoGEF antibody (ab110059) at 1/500 dilution

Lane 1:

A549 cell extract at 30 µg

Lane 2:

COLO cell extract at 30 µg

Lane 3:

COLO cell extract at 30 µg with immunizing peptide

Predicted band size: 168 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Synthetic Peptide within Human ARHGEF11. The exact immunogen used to generate this antibody is proprietary information.

O15085

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab110059 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PDZ-RhoGEF also known as ARHGEF11 functions as a guanine nucleotide exchange factor (GEF) that activates Rho GTPases through the exchange of GDP for GTP. This protein plays an important role in the regulation of actin cytoskeleton organization. PDZ-RhoGEF exists as a 170 kDa protein predominantly expressed in the brain heart and muscle tissues where it interacts with cellular membranes and the cytoskeleton. Its PDZ domain enables it to bind phospholipids and scaffold proteins contributing to its cellular localizations and function.
Biological function summary

PDZ-RhoGEF influences cell migration adhesion and proliferation by transmitting signals from G-protein-coupled receptors (GPCRs) to Rho proteins. This target forms part of a larger signaling complex commonly associating with the cytoplasmic tails of plexins and other membrane-associated proteins to facilitate its action. PDZ-RhoGEF's interaction with these partner proteins enables it to participate in controlling cellular dynamics particularly through modulating cytoskeletal structures.

Pathways

PDZ-RhoGEF integrates into critical signaling pathways including those involving Rho GTPase signaling and the Wnt signaling pathway. By interacting with RhoA a small GTPase PDZ-RhoGEF directs actin filament assembly which is essential for cellular processes such as motility and division. Additionally PDZ-RhoGEF connects with Dishevelled proteins within the Wnt pathway contributing to the regulation of cytoskeletal architecture and cellular polarity.

PDZ-RhoGEF's dysregulation links to conditions such as cancer and cardiovascular diseases. In cancer aberrant PDZ-RhoGEF activity can lead to uncontrolled cell migration and invasion often observed in metastatic processes. The interaction with proteins like AKT in signaling pathways further implicates PDZ-RhoGEF in oncogenic transformation. In cardiovascular diseases PDZ-RhoGEF contributes to vascular smooth muscle contraction with implications for hypertension and other vascular disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

May play a role in the regulation of RhoA GTPase by guanine nucleotide-binding alpha-12 (GNA12) and alpha-13 (GNA13). Acts as guanine nucleotide exchange factor (GEF) for RhoA GTPase and may act as GTPase-activating protein (GAP) for GNA12 and GNA13. Involved in neurotrophin-induced neurite outgrowth.
See full target information ARHGEF11
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Molecular biology of the cell 35:ar87 PubMed38656797

2024

Solo regulates the localization and activity of PDZ-RhoGEF for actin cytoskeletal remodeling in response to substrate stiffness.

Applications

Unspecified application

Species

Unspecified reactive species

Aoi Kunitomi,Shuhei Chiba,Nahoko Higashitani,Atsushi Higashitani,Shinichi Sato,Kensaku Mizuno,Kazumasa Ohashi

Science signaling 15:eabi9869 PubMed35041463

2022

A network of Gα signaling partners is revealed by proximity labeling proteomics analysis and includes PDZ-RhoGEF.

Applications

Unspecified application

Species

Unspecified reactive species

Naincy R Chandan,Saji Abraham,Shuvasree SenGupta,Carole A Parent,Alan V Smrcka

Journal of diabetes research 2019:6320839 PubMed31612150

2019

Transgenerational Obesity and Alteration of ARHGEF11 in the Rat Liver Induced by Intrauterine Hyperglycemia.

Applications

Unspecified application

Species

Unspecified reactive species

Wanyi Zhang,Rina Su,Hui Feng,Li Lin,Chen Wang,Huixia Yang

Placenta 63:7-14 PubMed29486856

2018

ARHGEF11 affecting the placental insulin signaling pathway in fetal macrosomia of normal glucose tolerance pregnant women.

Applications

WB, IHC-P

Species

Unspecified reactive species, Unspecified reactive species

Wanyi Zhang,Rina Su,Li Lin,Huixia Yang
View all publications
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