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AB235412

Anti-PDZD3 antibody

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(1 Publication)

Rabbit Polyclonal PDZD3 antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human NHERF4 aa 1-300.

View Alternative Names

IKEPP, PDZD3, PDZK2, DLNB27, NHERF4, Na(+)/H(+) exchange regulatory cofactor NHE-RF4, NHERF-4, Intestinal and kidney-enriched PDZ protein, Natrium-phosphate cotransporter IIa C-terminal-associated protein 2, PDZ domain-containing protein 2, PDZ domain-containing protein 3, Sodium-hydrogen exchanger regulatory factor 4, Na/Pi cotransporter C-terminal-associated protein 2, NaPi-Cap2

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDZD3 antibody (AB235412)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDZD3 antibody (AB235412)

Paraffin-embedded human skin tissue stained for PDZD3 using ab235412 at 1/100 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDZD3 antibody (AB235412)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDZD3 antibody (AB235412)

Paraffin-embedded human kidney tissue stained for PDZD3 using ab235412 at 1/100 dilution in immunohistochemical analysis.

Western blot - Anti-PDZD3 antibody (AB235412)
  • WB

Supplier Data

Western blot - Anti-PDZD3 antibody (AB235412)

All lanes:

Western blot - Anti-PDZD3 antibody (ab235412) at 1/500 dilution

Lane 1:

Mouse kidney tissue lysate

Lane 2:

Mouse heart tissue lysate

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/10000 dilution

Predicted band size: 61 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human NHERF4 aa 1-300. The exact immunogen used to generate this antibody is proprietary information.

Q86UT5

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purity >95%
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The PDZD3 protein also known as IKEPP or Inverted Formin IBP-Associated PDZ Protein functions as a scaffolding protein by containing multiple PDZ domains. This protein has a mass of approximately 72 kDa. PDZD3 is mostly expressed in the epithelial cells of the gastrointestinal tract particularly in the small intestine and colon. This specific expression pattern suggests its role in intestinal physiology.
Biological function summary

PDZD3 interacts with other proteins to modulate epithelial cell signaling and function. It does not work alone; instead it forms part of multi-protein complexes that oversee the cellular environment. These interactions often involve binding to the intracellular domains of transmembrane proteins influencing activities such as tight junction assembly and signal transduction.

Pathways

Scientists have identified PDZD3 as an important mediator in the regulation of the CFTR (Cystic Fibrosis Transmembrane Conductance Regulator) and other ion transport pathways. It interacts with proteins like NHERF1 and EBP50 impacting sodium and chloride ion regulation. Through these pathways PDZD3 plays a role in maintaining intestinal fluid balance and electrolyte homeostasis.

PDZD3 has notable implications for conditions such as cystic fibrosis and inflammatory bowel disease (IBD). In cystic fibrosis alterations in PDZD3 expression can disrupt CFTR function exacerbating symptoms. Furthermore in IBD changes in PDZD3 activity might contribute to impaired barrier function and inflammation with associated proteins like CFTR and NHERF1 often implicated in these pathophysiological processes.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Acts as a regulatory protein that associates with GUCY2C and negatively modulates its heat-stable enterotoxin-mediated activation (PubMed : 11950846). Stimulates SLC9A3 activity in the presence of elevated calcium ions (PubMed : 19088451).
See full target information NHERF4
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

iScience 26:106468 PubMed37091236

2023

Latent CSN-CRL complexes are crucial for curcumin-induced apoptosis and recruited during adipogenesis to lipid droplets via small GTPase RAB18.

Applications

Unspecified application

Species

Unspecified reactive species

Dawadschargal Dubiel,Jing Wang,Roland Hartig,Supattra Chaithongyot,Wolfgang Dubiel,Michael Naumann
View all publications
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Product promise

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