AB72467

PE Anti-6X His tag® antibody [AD1.1.10]

Name: PE conjugated Anti-6X His tag® antibody [AD1.1.10] (ab72467) | Abcam
Brand: Abcam Limited
SKU: AB72467
Price: 495 USD
Availability: InStock
Rating: 4 (2 reviews)

(2 Reviews)

(17 Publications)

Anti-6X His tag® antibody [AD1.1.10] - PE conjugated (ab72467) is a mouse monoclonal antibody detecting 6X His tag® in Flow Cytometry.

- Over 10 publications
- Trusted since 2008

View Alternative Names

6 His epitope tag, HHHHHH epitope tag, HHHHHH tag, Hexa His tag, Polyhistidine Tag, 6x his, 6x-HIS

1 Images

Flow Cytometry - PE Anti-6X His tag® antibody [AD1.1.10] (AB72467)

Flow Cyt

AbReview24202****

Flow Cytometry - PE Anti-6X His tag® antibody [AD1.1.10] (AB72467)

BALB-3T3 cells, which express moderate amounts of EGFR, were incubated with a His6-tagged anti-EGFR fibronectin domain. The labeled cells were washed and incubated with 50 microliters of 1 : 15 diluted PE-conjugated anti-His6 antibody. Cells were washed and analyzed by flow cytometry. A control without fibronectin domain was also analyzed. Image : Dashed black line is control without fibronectin. Solid blue line is sample with His6-tagged anti-EGFR fibronectin domain.

This image is courtesy of an Anonymous Abreview.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

AD1.1.10

Isotype

IgG1

Conjugation

Excitation/Emission

Ex: 480;565nm, Em: 578nm

Carrier free

Applications

Flow Cyt

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Tag": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/50 - 1/100", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/pe-mouse-igg1-b11-6-isotype-control-ab91357'>ab91357</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>" } } }

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Product details

What is this antibody validated in?
PE Anti-6X His tag® antibody [AD1.1.10] (ab72467) is a mouse monoclonal antibody and is validated for use in Flow Cytometry (Flow Cyt). Tagged protein detection
6X His tag® antibodies are used to visualize proteins labelled with this tag in a variety of applications.

Trusted by the scientific community
Anti-6X His tag® [AD1.1.10] PE (ab72467) was first used in a scientific publication in 2008 and has been cited over 10 times in peer-reviewed journals.

Related products
Antibody clone AD1.1.10 is also available pre-conjugated to a variety of labels for your convenience – Anti-6X His tag® PE [AD1.1.10] (ab72467).

Other related products
We have a range of other formats of antibody clone [AD1.1.10] also available for your convenience: ab15149, PE - ab72467, SureLight® APC - ab72579, Alkaline Phosphatase - ab81652, PerCP - ab117496, Carrier free - ab117504, DyLight® 550 - ab117507, DyLight® 488 - ab117512, HRP - ab178563, Carrier free - ab307236

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Properties and storage information

Form

Liquid

Purification technique

Size-exclusion chromatography

Purification notes

Size exclusion chromatography as well as some proprietary methods for purifying the final materials were used.

Storage buffer

pH: 7.4 Preservative: 0.013% Sodium azide Constituents: 1.71% Sucrose, 1.64% Sodium phosphate, 0.87% Sodium chloride, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The 6X His tag is a sequence commonly used for protein purification detection and immobilization. It consists of six histidine residues that bind to metal ions like nickel or cobalt. This makes it a highly efficient tag for affinity chromatography. His tag antibodies such as anti-His HRP facilitate the detection of His-tagged proteins in various assays. Being a small tag with a typical molecular weight of about 0.84 kDa it minimally interferes with protein function. Many proteins expressed in E. coli and other expression systems carry the 6X His tag for ease of purification and analysis.

Biological function summary

Proteins tagged with the 6X His tag often play roles in studies involving recombinant proteins. The tag itself does not alter the biological function of the target protein. It is not part of any natural protein complex but aids in the study of such complexes. Researchers use His tag antibodies like anti-His to specifically target and study these tagged proteins without cross-reactivity to other proteins.

Pathways

The incorporation of the 6X His tag into proteins allows them to be studied more efficiently in various biological pathways. It can be part of pathways like signal transduction or metabolic pathways. When tagged proteins interact with other molecules such as kinases or receptors researchers can track these interactions using the tag and anti-Histidine antibodies. This technique informs on proteins that are hard to purify due to low abundance or instability.

The 6X His tag itself does not have direct implications. However proteins of interest in disease models often carry the tag which allows researchers to purify and study them in detail. For example cancer-related proteins may be expressed with a His tag to understand their role in tumorigenesis. Similarly proteins involved in metabolic disorders could be tagged which helps in analyzing specific interactions or functions disrupted in diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information 6X His tag®

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Publications (17)

Recent publications for all applications. Explore the full list and refine your search

MedComm 4:e356 PubMed37701533

2023

Prime editor-mediated functional reshaping of prevents the entry of multiple human coronaviruses, including SARS-CoV-2 variants.

Applications

Unspecified application

Species

Unspecified reactive species

Wenwen Zhao,Jifang Li,Xiao Wang,Wei Xu,Bao-Qing Gao,Jiangchao Xiang,Yaofeng Hou,Wei Liu,Jing Wu,Qilian Qi,Jia Wei,Xiaoyu Yang,Lu Lu,Li Yang,Jia Chen,Bei Yang

Methods in molecular biology (Clifton, N.J.) 2682:245-258 PubMed37610587

2023

Assays for Detecting Henipavirus Antibodies.

Applications

Unspecified application

Species

Unspecified reactive species

Lin-Fa Wang,Shailendra Mani,Chee Wah Tan,Danielle E Anderson

The Journal of biological chemistry 298:102624 PubMed36272646

2022

A bovine antibody possessing an ultralong complementarity-determining region CDRH3 targets a highly conserved epitope in sarbecovirus spike proteins.

Applications

Unspecified application

Species

Unspecified reactive species

Matthew J Burke,James N F Scott,Thomas C Minshull,Zeqian Gao,Iain Manfield,Sinisa Savic,Peter G Stockley,Antonio N Calabrese,Joan Boyes

iScience 25:104431 PubMed35607524

2022

Structural and functional analysis of an inter-Spike bivalent neutralizing antibody against SARS-CoV-2 variants.

Applications

Unspecified application

Species

Unspecified reactive species

Yaning Li,Qing Fan,Bing Zhou,Yaping Shen,Yuanyuan Zhang,Lin Cheng,Furong Qi,Shuo Song,Yingying Guo,Renhong Yan,Bin Ju,Zheng Zhang

mAbs 14:2018960 PubMed35014603

2022

Beyond bispecificity: Controlled Fab arm exchange for the generation of antibodies with multiple specificities.

Applications

Unspecified application

Species

Unspecified reactive species

Desislava Yanakieva,Lukas Pekar,Andreas Evers,Markus Fleischer,Stephan Keller,Dirk Mueller-Pompalla,Lars Toleikis,Harald Kolmar,Stefan Zielonka,Simon Krah

Med (New York, N.Y.) 2:682-688.e4 PubMed33851143

2021

Early T cell and binding antibody responses are associated with COVID-19 RNA vaccine efficacy onset.

Applications

Unspecified application

Species

Unspecified reactive species

Shirin Kalimuddin,Christine Y L Tham,Martin Qui,Ruklanthi de Alwis,Jean X Y Sim,Joey M E Lim,Hwee-Cheng Tan,Ayesa Syenina,Summer L Zhang,Nina Le Bert,Anthony T Tan,Yan Shan Leong,Jia Xin Yee,Eugenia Z Ong,Eng Eong Ooi,Antonio Bertoletti,Jenny G Low

ACS omega 6:85-102 PubMed33458462

2021

Characterization of the SARS-CoV-2 S Protein: Biophysical, Biochemical, Structural, and Antigenic Analysis.

Applications

Unspecified application

Species

Unspecified reactive species

Natalia G Herrera,Nicholas C Morano,Alev Celikgil,George I Georgiev,Ryan J Malonis,James H Lee,Karen Tong,Olivia Vergnolle,Aldo B Massimi,Laura Y Yen,Alex J Noble,Mykhailo Kopylov,Jeffrey B Bonanno,Sarah C Garrett-Thomson,David B Hayes,Robert H Bortz,Ariel S Wirchnianski,Catalina Florez,Ethan Laudermilch,Denise Haslwanter,J Maximilian Fels,M Eugenia Dieterle,Rohit K Jangra,Jason Barnhill,Amanda Mengotto,Duncan Kimmel,Johanna P Daily,Liise-Anne Pirofski,Kartik Chandran,Michael Brenowitz,Scott J Garforth,Edward T Eng,Jonathan R Lai,Steven C Almo

Structure (London, England : 1993) 28:1197-1205.e2 PubMed32795404

2020

Redesigning HVEM Interface for Selective Binding to LIGHT, BTLA, and CD160.

Applications

Unspecified application

Species

Unspecified reactive species

Rojan Shrestha,Sarah C Garrett-Thomson,Weifeng Liu,Steven C Almo,Andras Fiser

bioRxiv : the preprint server for biology : PubMed32587972

2020

Characterization of the SARS-CoV-2 S Protein: Biophysical, Biochemical, Structural, and Antigenic Analysis.

Applications

Unspecified application

Species

Unspecified reactive species

Nature 584:115-119 PubMed32454513

2020

Human neutralizing antibodies elicited by SARS-CoV-2 infection.

Applications

Unspecified application

Species

Unspecified reactive species

Bin Ju,Qi Zhang,Jiwan Ge,Ruoke Wang,Jing Sun,Xiangyang Ge,Jiazhen Yu,Sisi Shan,Bing Zhou,Shuo Song,Xian Tang,Jinfang Yu,Jun Lan,Jing Yuan,Haiyan Wang,Juanjuan Zhao,Shuye Zhang,Youchun Wang,Xuanling Shi,Lei Liu,Jincun Zhao,Xinquan Wang,Zheng Zhang,Linqi Zhang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

PE Anti-6X His tag® antibody [AD1.1.10]

Flow Cytometry - PE Anti-6X His tag® antibody [AD1.1.10] (AB72467)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Applications

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (17)

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