AB237338

PE Anti-6X His tag® antibody [EPR20547]

RabMAb
Recombinant
What is this?

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(4 Publications)

Anti-6X His tag® antibody [EPR20547] - PE conjugated (ab237338) is a rabbit recombinant monoclonal antibody detecting 6X His tag® in Flow Cytometry (Intra), ICC/IF.

- Biophysical QC for unrivalled batch-batch consistency

2 Images

Immunocytochemistry/ Immunofluorescence - PE Anti-6X His tag® antibody [EPR20547] (AB237338)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - PE Anti-6X His tag® antibody [EPR20547] (AB237338)

Confocal image showing positive staining in HEK-293T cells transfected with 6X His tag with MYC tag® expression construct.

Flow Cytometry (Intracellular) - PE Anti-6X His tag® antibody [EPR20547] (AB237338)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - PE Anti-6X His tag® antibody [EPR20547] (AB237338)

Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized HEK-293T cells transfected with 6X His tag with MYC tag with ab237338 at 1/500 dilution (Right panel) compared with Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Phycoerythrin) (ab209478) (Left panel).

Blocking with 10% Goat serum.

519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-6X His tag® antibody [EPR20547]
HRP

HRP Anti-6X His tag® antibody [EPR20547]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-6X His tag® antibody [EPR20547]
Biotin

Biotin Anti-6X His tag® antibody [EPR20547] - ChIP Grade
660 APC

APC Anti-6X His tag® antibody [EPR20547] - ChIP Grade
519 FITC

FITC Anti-6X His tag® [EPR20547]
Unconjugated

Anti-6X His tag® antibody [EPR20547] - ChIP Grade
Carrier free

Anti-6X His tag® antibody [EPR20547] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR20547

Isotype

IgG

Conjugation

Excitation/Emission

Ex: 480;565nm, Em: 578nm

Carrier free

Applications

ICC/IF, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Tag": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/500", "FlowCytIntra-species-notes": "<p></p>" } } }

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Product details

What is this antibody validated in?
PE Anti-6X His tag® antibody [EPR20547] (ab237338) is a rabbit recombinant monoclonal antibody and is validated for use in Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunocytochemistry/immunofluorescence (ICC/IF). Tagged protein detection
6X His tag® antibodies are used to visualize proteins labelled with this tag in a variety of applications.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Related products
Antibody clone EPR20547 is also available pre-conjugated to a variety of labels for your convenience – Anti-6X His tag® PE [EPR20547] (ab237338).

Other related products
We have a range of other formats of antibody clone [EPR20547] also available for your convenience: ab213204, Carrier free - ab232492, Alexa Fluor® 488 - ab237336, Alexa Fluor® 647 - ab237337, PE - ab237338, HRP - ab237339, Biotin - ab316173, APC - ab317659, FITC - ab318311

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Store in the dark

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The 6X His tag is a sequence commonly used for protein purification detection and immobilization. It consists of six histidine residues that bind to metal ions like nickel or cobalt. This makes it a highly efficient tag for affinity chromatography. His tag antibodies such as anti-His HRP facilitate the detection of His-tagged proteins in various assays. Being a small tag with a typical molecular weight of about 0.84 kDa it minimally interferes with protein function. Many proteins expressed in E. coli and other expression systems carry the 6X His tag for ease of purification and analysis.

Biological function summary

Proteins tagged with the 6X His tag often play roles in studies involving recombinant proteins. The tag itself does not alter the biological function of the target protein. It is not part of any natural protein complex but aids in the study of such complexes. Researchers use His tag antibodies like anti-His to specifically target and study these tagged proteins without cross-reactivity to other proteins.

Pathways

The incorporation of the 6X His tag into proteins allows them to be studied more efficiently in various biological pathways. It can be part of pathways like signal transduction or metabolic pathways. When tagged proteins interact with other molecules such as kinases or receptors researchers can track these interactions using the tag and anti-Histidine antibodies. This technique informs on proteins that are hard to purify due to low abundance or instability.

The 6X His tag itself does not have direct implications. However proteins of interest in disease models often carry the tag which allows researchers to purify and study them in detail. For example cancer-related proteins may be expressed with a His tag to understand their role in tumorigenesis. Similarly proteins involved in metabolic disorders could be tagged which helps in analyzing specific interactions or functions disrupted in diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information 6X His tag®

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Communications biology 8:1322 PubMed40885856

2025

Engineering virus-like particles for safe and versatile modeling of SARS-CoV-2 host interaction and immune escape.

Applications

Unspecified application

Species

Unspecified reactive species

Liang Ma,Yuan Lin,Siyu Ma,Kai Chen,Yihan Lin

Molecular medicine (Cambridge, Mass.) 31:170 PubMed40325370

2025

Bacteriophage-derived endolysins restore antibiotic susceptibility in β-lactam- and macrolide-resistant Streptococcus pneumoniae infections.

Applications

Unspecified application

Species

Unspecified reactive species

Niels Vander Elst,Kristine Farmen,Lisa Knörr,Lotte Merlijn,Federico Iovino

Journal of medical virology 95:e28642 PubMed36890630

2023

Pre-existing immunity to SARS-CoV-2 associates with strong T cell responses induced by inactivated COVID-19 vaccines.

Applications

Unspecified application

Species

Unspecified reactive species

Xuan Yi,Yuhao Wang,Quanrun Li,Xiaoyi Li,Panli Zhang,Xin Fu,Shuqin Gu,Daqian Zhang,Xiaoyi Liu,Haonan Lou,Yuemei Wu,Libo Tang,Jinlin Hou,Yongyin Li

International journal of molecular sciences 24: PubMed36835603

2023

4-1BB-Based CAR T Cells Effectively Reverse Exhaustion and Enhance the Anti-Tumor Immune Response through Autocrine PD-L1 scFv Antibody.

Applications

Unspecified application

Species

Unspecified reactive species

Kang Cheng,Xiangming Feng,Zhirong Chai,Zhenzhen Wang,Zheng Liu,Zhanchao Yan,Yanming Wang,Shoutao Zhang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

PE Anti-6X His tag® antibody [EPR20547]

Immunocytochemistry/ Immunofluorescence - PE Anti-6X His tag® antibody [EPR20547] (AB237338)

Flow Cytometry (Intracellular) - PE Anti-6X His tag® antibody [EPR20547] (AB237338)

Related conjugates and formulations (8)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Applications

Immunogen

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Product promise