JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB212001

PE Anti-ABCE1 antibody [EPR15373(B)] - C-terminal

Be the first to review this product! Submit a review

|

(0 Publication)

Rabbit Recombinant Monoclonal ABCE1 antibody - conjugated to PE. C-terminal. Suitable for Flow Cyt (Intra), ICC/IF, IHC-P and reacts with Human, Mouse, Rat samples.

View Alternative Names

RLI, RNASEL1, RNASELI, RNS4I, OK/SW-cl.40, ABCE1, ATP-binding cassette sub-family E member 1, 2'-5'-oligoadenylate-binding protein, HuHP68, RNase L inhibitor, Ribonuclease 4 inhibitor

1 Images
Flow Cytometry (Intracellular) - PE Anti-ABCE1 antibody [EPR15373(B)] - C-terminal (AB212001)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - PE Anti-ABCE1 antibody [EPR15373(B)] - C-terminal (AB212001)

Overlay histogram showing K562 cells stained with ab212001 (red line). The cells were fixed with 4 % formaldehyde (10 min) and then permeabilized with 0.1 % PBS-Triton X-100 for 15 min. The cells were incubated in 1x PBS containing 10 % normal goat serum to block non-specific protein-protein interaction followed by the antibody (ab212001) (1x 106 in 100μl at 1 μg/ml (1/500)) for 30 min at 22°C.

Isotype control antibody (black line) was Rabbit IgG (monoclonal) Phycoerythrin (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

Acquisition of >5,000 events were collected using a 50 mW Yellow/Green laser (561nm) and 586/15 bandpass filter.

This antibody gave a positive signal in K562 cells fixed with 80 % methanol (5 min) / permeabilized with 0.1 % PBS-Triton X-100 for 15 min used under the same conditions.

  • HRP

    HRP Anti-ABCE1 antibody [EPR15373(B)] - C-terminal

  • Unconjugated

    Anti-ABCE1 antibody [EPR15373(B)] - C-terminal

  • Carrier free

    Anti-ABCE1 antibody [EPR15373(B)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR15373(B)

Isotype

IgG

Conjugation

PE

Excitation/Emission

Ex: 480;565nm, Em: 578nm

Carrier free

No

Reacts with

Human, Human, Mouse, Rat

Applications

IHC-P, Flow Cyt (Intra), ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Complementary Products

Primary Antibodies

AB246750

Alexa Fluor® 647 Anti-Filamin A antibody [EP2405Y]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-Filamin A antibody [EP2405Y] (AB246750)

  • 0
  • 0
Primary Antibodies

AB252062

Alexa Fluor® 488 Anti-RPL10A antibody [EPR12344]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-RPL10A antibody [EPR12344] (AB252062)

  • 0
  • 0
Primary Antibodies

AB252072

Alexa Fluor® 488 Anti-MetRS/MARS antibody [EPR9873(B)]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-MetRS/MARS antibody [EPR9873(B)] (AB252072)

  • 0
  • 0
Primary Antibodies

AB237256

HRP Anti-Ubiquitin (linkage-specific K27) antibody [EPR17034]

RabMAb|Recombinant

Western blot - HRP Anti-Ubiquitin (linkage-specific K27) antibody [EPR17034] (AB237256)

  • 0
  • 0
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/500", "FlowCytIntra-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Rat": { "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
style
left-column

Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Do Not Freeze|Store in the dark
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ABCE1 also known as ATP-binding cassette sub-family E member 1 is an ATPase involved in various cellular processes. It belongs to the ATP-binding cassette (ABC) transporter family. The protein mass is approximately 68 kDa and its expression occurs widely in various tissues including the cytoplasm and mitochondria. ABCE1 has a unique structure with two nucleotide-binding domains making it distinct from other ABC family members that typically transport molecules across membranes.
Biological function summary

ABCE1 plays a role in inhibiting ribonuclease activity and is essential in ribosome recycling. This protein interacts with translation initiation factors and actively participates in the dissociation of ribosomes after translation termination. It is a part of the ribosome recycling complex an important multi-proteins setup necessary to prepare ribosomes for another round of translation. The biochemical interactions and presence in different cellular compartments highlight its multiple roles in cellular homeostasis.

Pathways

ABCE1 engages significantly in translation and its regulation. It plays an important role in the translation termination pathway where it interacts with eukaryotic release factors. Additionally it influences the degradation pathway of defective ribosomal products. Within these pathways the function of ABCE1 is closely linked to proteins like eRF1 and eRF3 which are essential in the translation termination process. The interaction of these proteins ensures efficient termination and recycling of translation machinery components.

Alterations in ABCE1 expression or function link to certain cancers and viral infections. The aberrant activity of ABCE1 influences cancer progression as it affects protein synthesis and cellular growth levels. Also ABCE1 has been implicated in promoting the replication of certain viruses providing them a competitive advantage. Proteins like eIF3 and eIF4 involved in translation initiation can show altered associations in disorders where ABCE1 is dysregulated influencing disease outcomes and progression.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Nucleoside-triphosphatase (NTPase) involved in ribosome recycling by mediating ribosome disassembly (PubMed : 20122402, PubMed : 21448132). Able to hydrolyze ATP, GTP, UTP and CTP (PubMed : 20122402). Splits ribosomes into free 60S subunits and tRNA- and mRNA-bound 40S subunits (PubMed : 20122402, PubMed : 21448132). Acts either after canonical termination facilitated by release factors (ETF1/eRF1) or after recognition of stalled and vacant ribosomes by mRNA surveillance factors (PELO/Pelota) (PubMed : 20122402, PubMed : 21448132). Involved in the No-Go Decay (NGD) pathway : recruited to stalled ribosomes by the Pelota-HBS1L complex, and drives the disassembly of stalled ribosomes, followed by degradation of damaged mRNAs as part of the NGD pathway (PubMed : 21448132). Also plays a role in quality control of translation of mitochondrial outer membrane-localized mRNA (PubMed : 29861391). As part of the PINK1-regulated signaling, ubiquitinated by CNOT4 upon mitochondria damage; this modification generates polyubiquitin signals that recruit autophagy receptors to the mitochondrial outer membrane and initiate mitophagy (PubMed : 29861391). RNASEL-specific protein inhibitor which antagonizes the binding of 2-5A (5'-phosphorylated 2',5'-linked oligoadenylates) to RNASEL (PubMed : 9660177). Negative regulator of the anti-viral effect of the interferon-regulated 2-5A/RNASEL pathway (PubMed : 11585831, PubMed : 9660177, PubMed : 9847332).. (Microbial infection) May act as a chaperone for post-translational events during HIV-1 capsid assembly.. (Microbial infection) Plays a role in the down-regulation of the 2-5A/RNASEL pathway during encephalomyocarditis virus (EMCV) and HIV-1 infections.
See full target information ABCE1
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com