AB209435

PE Anti-alpha smooth muscle Actin (acetyl E3) + ACTG2 (acetyl E3) antibody [E184]

Name: Anti-alpha smooth muscle Actin (acetyl E3) + ACTG2 (acetyl E3) PE antibody [E184] (ab209435) | Abcam
Brand: Abcam Limited
SKU: AB209435
Price: 620 USD
Availability: InStock
Rating: 5 (1 reviews)

RabMAb
Recombinant
What is this?

(1 Review)

(6 Publications)

Rabbit Recombinant Monoclonal alpha smooth muscle Actin acetyl E3 antibody - conjugated to PE. Suitable for ICC/IF, Flow Cyt (Intra) and reacts with Rat, Human samples. Cited in 6 publications.

View Alternative Names

ACTSA, ACTVS, GIG46, ACTA2, Alpha-actin-2, Cell growth-inhibiting gene 46 protein

2 Images

Flow Cytometry (Intracellular) - PE Anti-alpha smooth muscle Actin (acetyl E3) + ACTG2 (acetyl E3) antibody [E184] (AB209435)

Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - PE Anti-alpha smooth muscle Actin (acetyl E3) + ACTG2 (acetyl E3) antibody [E184] (AB209435)

Overlay histogram showing SV40LT-SMC cells stained with ab209435 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab209435, 1/5000 dilution) for 30 min at 22°C.

Isotype control antibody (black line) was rabbit IgG (monoclonal) Phycoerythrin (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

Acquisition of >5,000 events were collected using a 20 mW Solid State Blue Laser (488nm) and 585/40 bandpass filter.

This antibody gave a positive signal in SV40LT-SMC cells fixed with 4% formaldehyde (10 min)/permeabilized with 0.1% PBS-Triton X-100 for 15 min used under the same conditions.

Immunocytochemistry/ Immunofluorescence - PE Anti-alpha smooth muscle Actin (acetyl E3) + ACTG2 (acetyl E3) antibody [E184] (AB209435)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - PE Anti-alpha smooth muscle Actin (acetyl E3) + ACTG2 (acetyl E3) antibody [E184] (AB209435)

ab209435 staining alpha smooth muscle Actin in SV40LT-SMC cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab209435 at 1/500 dilution (Pseudocolored in green) and ab195884, Rat monoclonal to Tubulin (Alexa Fluor^® 647), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

519 FITC

FITC Anti-alpha smooth muscle Actin (acetyl E3) + ACTG2 (acetyl E3) antibody [E184]
421 Alexa Fluor® 405

Alexa Fluor® 405 Anti-alpha smooth muscle Actin (acetyl E3) + ACTG2 (acetyl E3) antibody [E184]
HRP

HRP Anti-alpha smooth muscle Actin (acetyl E3) + ACTG2 (acetyl E3) antibody [E184]
660 APC

APC Anti-alpha smooth muscle Actin (acetyl E3) + ACTG2 (acetyl E3) antibody [E184]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-alpha smooth muscle Actin (acetyl E3) + ACTG2 (acetyl E3) antibody [E184]
Unconjugated

Anti-alpha smooth muscle Actin (acetyl E3) + ACTG2 (acetyl E3) antibody [E184]
Carrier free

Anti-alpha smooth muscle Actin (acetyl E3) + ACTG2 (acetyl E3) antibody [E184] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

E184

Isotype

IgG

Conjugation

Excitation/Emission

Ex: 480;565nm, Em: 578nm

Carrier free

Reacts with

Rat, Human

Applications

ICC/IF, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

The antibody has been shown to detect P62736-ACTA (gene ACTA2): (Ac)-EEEDSTALVC and P63267-ACTH (gene ACTG2): (Ac)-EEETTALVC in indirect ELISA.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "This product gave a positive signal in SV40LT cells fixed with 4% formaldehyde (10 min)", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "This product gave a positive signal in SV40LT cells fixed with 4% formaldehyde (10 min)", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/5000", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/pe-rabbit-igg-monoclonal-epr25a-isotype-control-ab209478'>ab209478</a> - Rabbit monoclonal IgG (Phycoerythrin), is suitable for use as an isotype control with this antibody." } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Do Not Freeze|Store in the dark

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Alpha smooth muscle Actin also known as ACTG2 is an important protein in muscle contraction and cellular structure. It is most commonly found in smooth muscle cells and is not typically expressed in striated muscle. This protein is part of the actin family and has a molecular mass of approximately 42 kDa. ACTG2 plays a role in forming microfilaments which are key components of the muscle contractile machinery. In smooth muscle tissues such as those found in the intestines and blood vessels ACTG2 is often detected together with other cytoskeletal and regulatory proteins.

Biological function summary

ACTG2 contributes to muscle contraction by facilitating the interaction between actin and myosin. It is not considered a part of a protein complex but interacts closely with several other proteins involved in contractile functions. Beyond contraction ACTG2 also supports cellular integrity and shape maintenance. In tissues ACTG2 is involved in activities that require dynamic structural reorganization such as during cellular migration and wound healing highlighting its versatility beyond just a contractile agent.

Pathways

ACTG2 participates in the regulation of the smooth muscle contraction pathway influencing muscle tone and responsiveness to stimuli. The actin-myosin interaction where ACTG2 is involved is critical for the contractile process with other proteins like myosin light chain kinase (MLCK) and tropomyosin playing supportive roles. Additionally the integrin signaling pathway is another context where ACTG2 is indirectly involved aiding in the transmission of mechanical signals from the extracellular matrix to the cell.

ACTG2 is associated with conditions such as megacystis-microcolon-intestinal hypoperistalsis syndrome (MMIHS) and various vascular disorders. Mutations in the ACTG2 gene can disrupt normal muscle function leading to defective peristalsis in the gastrointestinal tract as seen in MMIHS. The connection between ACTG2 and myosin regulatory light chains illustrates how changes in its function can impact muscle contractility and lead to disease. In vascular disorders abnormal expression of ACTG2 is often linked to impaired vessel tone and blood flow regulation.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.

See full target information ACTA2 acetyl E3

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 12:e2410229 PubMed39804980

2025

Molecular Determinants of Protein Pathogenicity at the Single-Aggregate Level.

Applications

Unspecified application

Species

Unspecified reactive species

Agnieszka Urbanek,Emma F Garland,Emily E Prescott,Marianne C King,Anna Olerinyova,Hollie E Wareing,Nia Georgieva,Ellie L Bradshaw,Svetomir B Tzokov,Alexander Knight,Alexander I Tartakovskii,Tarja Malm,J Robin Highley,Suman De

Immunity 57:1306-1323.e8 PubMed38815582

2024

Intestinal CXCR6 ILC3s migrate to the kidney and exacerbate renal fibrosis via IL-23 receptor signaling enhanced by PD-1 expression.

Applications

Unspecified application

Species

Unspecified reactive species

Zhou Liang,Ziwen Tang,Changjian Zhu,Feng Li,Shuaijiabin Chen,Xu Han,Ruilin Zheng,Xinrong Hu,Ruoni Lin,Qiaoqiao Pei,Changjun Yin,Ji Wang,Ce Tang,Nan Cao,Jincun Zhao,Rong Wang,Xiaoyan Li,Ning Luo,Qiong Wen,Jianwen Yu,Jianbo Li,Xi Xia,Xunhua Zheng,Xin Wang,Naya Huang,Zhong Zhong,Chengqiang Mo,Peisong Chen,Yating Wang,Jinjin Fan,Yun Guo,Haojie Zhong,Jiaqi Liu,Zhenwei Peng,Haiping Mao,Guo-Ping Shi,Joseph V Bonventre,Wei Chen,Yi Zhou

Scientific reports 12:17605 PubMed36266453

2022

Angiogenic stem cell delivery platform to augment post-infarction neovasculature and reverse ventricular remodeling.

Applications

Unspecified application

Species

Unspecified reactive species

Hye Sook Shin,Akshara Thakore,Yuko Tada,Albert J Pedroza,Gentaro Ikeda,Ian Y Chen,Doreen Chan,Kevin J Jaatinen,Shin Yajima,Eric M Pfrender,Masashi Kawamura,Phillip C Yang,Joseph C Wu,Eric A Appel,Michael P Fischbein,YJoseph Woo,Yasuhiro Shudo

Journal of the American Heart Association 11:e023695 PubMed35348006

2022

Wnt Site Signaling Inhibitor Secreted Frizzled-Related Protein 3 Protects Mitral Valve Endothelium From Myocardial Infarction-Induced Endothelial-to-Mesenchymal Transition.

Applications

Unspecified application

Species

Unspecified reactive species

Zahra Alvandi,Yasufumi Nagata,Livia Silva Araúujo Passos,Ali Hashemi Gheinani,J Luis Guerrero,Jill Wylie-Sears,Dayana Carolina Romero,Brittan A Morris,Suzanne M Sullivan,Koushiar M Yaghoubian,Amirhossein Alvandi,Rosalyn M Adam,Elena Aikawa,Robert A Levine,Joyce Bischoff

Aging 13:15523-15537 PubMed34099591

2021

S100A8 and S100A9, both transcriptionally regulated by PU.1, promote epithelial-mesenchymal transformation (EMT) and invasive growth of dermal keratinocytes during scar formation post burn.

Applications

Unspecified application

Species

Unspecified reactive species

Zhigang Xu,Chuantao Cheng,Ranran Kong,Yale Liu,Shuang Wang,Yuefeng Ma,Xin Xing

European journal of pharmacology 887:173590 PubMed32976827

2020

Post-infarct morphine treatment reduces apoptosis and myofibroblast density in a rat model of cardiac ischemia-reperfusion.

Applications

Unspecified application

Species

Unspecified reactive species

Sulail Fatima Rajani,Mahdieh Faghihi,Alireza Imani

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

PE Anti-alpha smooth muscle Actin (acetyl E3) + ACTG2 (acetyl E3) antibody [E184]

Flow Cytometry (Intracellular) - PE Anti-alpha smooth muscle Actin (acetyl E3) + ACTG2 (acetyl E3) antibody [E184] (AB209435)

Immunocytochemistry/ Immunofluorescence - PE Anti-alpha smooth muscle Actin (acetyl E3) + ACTG2 (acetyl E3) antibody [E184] (AB209435)

Related conjugates and formulations (7)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (6)

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