JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB318410

PE Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656-290]

Be the first to review this product! Submit a review

|

(0 Publication)

Rabbit Recombinant Monoclonal Androgen Receptor antibody - conjugated to PE.

View Alternative Names

DHTR, NR3C4, AR, Androgen receptor, Dihydrotestosterone receptor, Nuclear receptor subfamily 3 group C member 4

  • Unconjugated

    Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656-290]

  • Carrier free

    Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656-290] - BSA and Azide free

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656-290]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656-290]

  • 660 APC

    APC Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656-290]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656-290]

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656-290]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR15656-290

Isotype

IgG

Conjugation

PE

Excitation/Emission

Ex: 480;565nm, Em: 578nm

Carrier free

No

Applications

Antibody Labelling, Target Binding Affinity

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

How are conjugated primary antibodies validated?
This conjugated primary antibody is released using a quantitative quality control method that evaluates binding affinity post-conjugation and efficiency of antibody labeling.
For suitable applications and species reactivity, please refer to the unconjugated version of this clone.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Androgen Receptor (AR-V7 specific) is a variant of the androgen receptor a type of nuclear receptor critical for mediating the effects of androgens like testosterone. AR-V7 is a protein that lacks the ligand-binding domain present in full-length androgen receptors resulting in its activity being independent of androgen presence. It is prominently expressed in prostate tissues and is often observed in prostate cancer cells resistant to conventional therapies. The protein variant exhibits a molecular mass of about 75 kDa. Commonly recognized aliases for AR-V7 include AR-V7 ARv7 and AR V7.
Biological function summary

The AR-V7 is significant in cellular proliferation and survival processes without needing direct androgen binding due to its interaction capability with specific nuclear elements. It resides in the cell nucleus and engages with DNA as part of a transcriptional regulatory complex. Its ability to modulate the transcription of target genes makes it a prominent player in the regulation of cell cycle and apoptosis. In many instances AR-V7 forms complexes with other transcriptional modulators and nuclear proteins that augment its transcriptional activity.

Pathways

AR-V7 plays an important role in the androgen signaling pathway which is pivotal in the progression and development of prostate cancer. It interacts with various co-regulators and influences the expression of genes involved in cell growth and differentiation. The splice variant also participates in other related signaling pathways such as the PI3K/AKT pathway indirectly interfacing with proteins like AR peptides and AR protein which further highlights its relevance in intracellular signaling mechanisms.

AR-V7 has a well-established connection with castration-resistant prostate cancer (CRPC) a form of prostate cancer that progresses despite androgen deprivation therapy. The presence of AR-V7 signifies a poor prognosis and resistance to anti-androgen therapies. Its transcriptional activity is linked to proteins such as AR protein and anti-androgen agents that typically target the full-length androgen receptor. Understanding the role of AR-V7 in reinforcing cancer cell survival and therapy resistance has fueled research towards developing targeted therapeutics and diagnostics like AR-V7 ELISA kits and AR IHC assays.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

The protein expressed by the AR gene functions as a steroid hormone receptor, a type of ligand-activated transcription factor that regulates eukaryotic gene expression and influences cellular proliferation and differentiation in target tissues. Its transcription factor activity can be modulated by coactivator and corepressor proteins, such as ZBTB7A, which recruits NCOR1 and NCOR2 to androgen response elements on target genes, thereby negatively regulating androgen receptor signaling and androgen-induced cell proliferation. The transcription activation can also be down-regulated by NR0B2. The protein is activated, though not phosphorylated, by HIPK3 and ZIPK/DAPK3. Notably, isoforms 3 and 4 of this protein lack the C-terminal ligand-binding domain and may thus constitutively activate transcription of specific gene sets independently of steroid hormones. This supplementary information is collated from multiple sources and compiled automatically.
See full target information AR
style
left-column
style
left-column
style
right-column

Complementary Products

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Biochemicals

AB141924

Cytarabine, Pyrimidine nucleoside analog

Chemical Structure - Cytarabine, Pyrimidine nucleoside analog (AB141924)

  • 0
  • 0
Proteins & Peptides

AB235857

Recombinant Human Androgen Receptor protein (Tagged)

SDS-PAGE - Recombinant Human Androgen Receptor protein (Tagged) (AB235857)

  • 0
  • 0
Primary Antibodies

AB15580

Anti-Ki67 antibody

BOND RX™ Validated|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody (AB15580)

  • 5
  • 230

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com