AB210729

PE Anti-Annexin-2/ANXA2 antibody [EPR13052(B)]

RabMAb
Recombinant
What is this?

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(2 Publications)

Rabbit Recombinant Monoclonal Annexin-2/ANXA2 antibody - conjugated to PE. Suitable for Flow Cyt (Intra), ICC/IF, IHC-P and reacts with Human samples. Cited in 2 publications.

View Alternative Names

ANX2, ANX2L4, CAL1H, LPC2D, ANXA2, Annexin A2, Annexin II, Annexin-2, Calpactin I heavy chain, Calpactin-1 heavy chain, Chromobindin-8, Lipocortin II, Placental anticoagulant protein IV, Protein I, p36, PAP-IV

1 Images

Flow Cytometry (Intracellular) - PE Anti-Annexin-2/ANXA2 antibody [EPR13052(B)] (AB210729)

Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - PE Anti-Annexin-2/ANXA2 antibody [EPR13052(B)] (AB210729)

Overlay histogram showing HeLa cells stained with ab210729 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 90% methanol for 30 min at -20°C. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab210729, 1/5000 dilution) for 30 min at 22°C.

Isotype control antibody (black line) was rabbit IgG (monoclonal) Phycoerythrin (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

Acquisition of >5,000 events were collected using a 50 mW Yellow/Green laser (561nm) and 586/15 bandpass filter.

617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-Annexin-2/ANXA2 antibody [EPR13052(B)]
HRP

HRP Anti-Annexin-2/ANXA2 antibody [EPR13052(B)]
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-Annexin-2/ANXA2 antibody [EPR13052(B)]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-Annexin-2/ANXA2 antibody [EPR13052(B)]
Unconjugated

Anti-Annexin-2/ANXA2 antibody [EPR13052(B)]
Carrier free

Anti-Annexin-2/ANXA2 antibody [EPR13052(B)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13052(B)

Isotype

IgG

Conjugation

Excitation/Emission

Ex: 480;565nm, Em: 578nm

Carrier free

Reacts with

Human, Human

Applications

IHC-P, Flow Cyt (Intra), ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/5000", "FlowCytIntra-species-notes": "The cellular localisation of this product has been verified in ICC/IF", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Do Not Freeze|Store in the dark

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Annexin-2 also known as ANXA2 is a multifunctional protein of approximately 36 kDa. It belongs to the annexin family and is known for its strong calcium-dependent interactions with phospholipid membranes. Commonly expressed in endothelial cells it also appears in various tissues including epithelial cells and certain types of cancer cells. ANXA2 forms a heterotetramer complex with S100A10 also known as p11 which modulates its availability and function. Other names for ANXA2 include its gene designation M298 and its role in complexes often goes by ANXA2R.

Biological function summary

Annexin-2 acts beyond mere cellular structural support. It plays a part in fibrinolysis by acting as a co-receptor for plasminogen and tissue plasminogen activator promoting plasmin generation. The protein is also implicated in membrane trafficking signal transduction and organization of microdomains on cellular surfaces. As part of a complex with S100A10 ANXA2 can bind to cytoskeletal elements facilitating vesicle trafficking and influencing cell shape and motility. This complex assembly hints at ANXA2's importance in maintaining cellular dynamism and interplay with external signals.

Pathways

ANXA2 is integral in the regulation of the plasminogen activation pathway important for fibrinolytic activity. It also participates in the signal transduction pathways influencing cellular responses to stress and growth factors. ANXA2 interacts with various proteins like Src kinase and is involved in processes like the EGFR signaling pathway. This protein often crosstalks with the cytoskeletal network enabling the coupling of membrane dynamics with intracellular signaling cascades.

Annexin-2's dysregulation associates with conditions such as cancer and thrombosis. In cancer overexpression of ANXA2 links with tumor progression invasion and metastasis acting synergistically with proteins like Src to promote oncogenic pathways. In thrombosis ANXA2’s role in fibrinolysis becomes impaired contributing to excessive clot formation. Studies also indicate interactions with plasminogen-related proteins as being central to understanding its pathological roles in these diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Calcium-regulated membrane-binding protein whose affinity for calcium is greatly enhanced by anionic phospholipids. It binds two calcium ions with high affinity. May be involved in heat-stress response. Inhibits PCSK9-enhanced LDLR degradation, probably reduces PCSK9 protein levels via a translational mechanism but also competes with LDLR for binding with PCSK9 (PubMed : 18799458, PubMed : 22848640, PubMed : 24808179). Binds to endosomes damaged by phagocytosis of particulate wear debris and participates in endosomal membrane stabilization, thereby limiting NLRP3 inflammasome activation (By similarity). Required for endothelial cell surface plasmin generation and may support fibrinolytic surveillance and neoangiogenesis (By similarity).. (Microbial infection) Binds M.pneumoniae CARDS toxin, probably serves as one receptor for this pathogen. When ANXA2 is down-regulated by siRNA, less toxin binds to human cells and less vacuolization (a symptom of M.pneumoniae infection) is seen.

See full target information ANXA2

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Virology 573:50-58 PubMed35714458

2022

Integrated proteomics and transcriptomics analyses identify novel cell surface markers of HIV latency.

Applications

Unspecified application

Species

Unspecified reactive species

Nadejda Beliakova-Bethell,Antigoni Manousopoulou,Savitha Deshmukh,Amey Mukim,Douglas D Richman,Spiros D Garbis,Celsa A Spina

Science advances 7: PubMed34380620

2021

Protein detection in blood with single-molecule imaging.

Applications

Unspecified application

Species

Unspecified reactive species

Chih-Ping Mao,Shih-Chin Wang,Yu-Pin Su,Ssu-Hsueh Tseng,Liangmei He,Annie A Wu,Richard B S Roden,Jie Xiao,Chien-Fu Hung

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

PE Anti-Annexin-2/ANXA2 antibody [EPR13052(B)]

Flow Cytometry (Intracellular) - PE Anti-Annexin-2/ANXA2 antibody [EPR13052(B)] (AB210729)

Related conjugates and formulations (6)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise