JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB278736

PE Anti-ASK1 (phospho S83) antibody [Ask1S83-G4]

Be the first to review this product! Submit a review

|

(1 Publication)

Rabbit Recombinant Monoclonal ASK1 phospho S83 antibody - conjugated to PE. Suitable for Flow Cyt and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human MAP3K5 phospho S83.

View Alternative Names

ASK1, MAPKKK5, MEKK5, MAP3K5, Mitogen-activated protein kinase kinase kinase 5, Apoptosis signal-regulating kinase 1, MAPK/ERK kinase kinase 5, ASK-1, MEK kinase 5, MEKK 5

1 Images
Flow Cytometry - PE Anti-ASK1 (phospho S83) antibody [Ask1S83-G4] (AB278736)
  • Flow Cyt

Supplier Data

Flow Cytometry - PE Anti-ASK1 (phospho S83) antibody [Ask1S83-G4] (AB278736)

Flow cytometric analysis of HT1080 human fibrosarcoma unstained cells, treated with imatinib as negative control (blue), or treated with imatinib (red) or treated with pervanadate (green) and stained using ab278736.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

Ask1S83-G4

Isotype

IgG

Light chain type

kappa

Conjugation

PE

Excitation/Emission

Ex: 480;565nm, Em: 578nm

Carrier free

No

Reacts with

Human

Applications

Flow Cyt

applications

Immunogen

Synthetic Peptide within Human MAP3K5 phospho S83. The exact immunogen used to generate this antibody is proprietary information.

Q99683

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "5 µL for 10^6 Cells", "FlowCyt-species-notes": "<p></p>" } } }
style
left-column

Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A/G
Storage buffer
Preservative: 0.09% Sodium azide Constituents: PBS, 0.2% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Store in the dark
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The ASK1 protein also known as apoptosis signal-regulating kinase 1 is an important component in cellular stress response pathways. This 150 kDa protein is a member of the mitogen-activated protein kinase kinase kinase (MAP3K) family. ASK1 is expressed in several tissues including the heart brain liver and pancreas. Mechanically ASK1 gets activated in response to a variety of stress stimuli such as oxidative stress and endoplasmic reticulum stress which significantly impact the regulation of programmed cell death.
Biological function summary

In response to stressors ASK1 activates the c-Jun N-terminal kinase (JNK) and p38 MAPK signaling pathways which play roles in apoptosis and inflammation. ASK1 tends to form a complex with TRAF2 and TRAF6 key adapters in downstream signaling. This kinase acts as an important link bridging environmental stress signals to cellular responses. Through its function ASK1 influences cell fate decisions in response to various stress conditions.

Pathways

ASK1 is a central player in the MAPK signaling cascade. Within this cascade ASK1 regulates JNK and p38 MAPK pathways which are significant in stress adaptation and cellular responses. ASK1 interacts with proteins like MKK4 and MKK7 in the JNK pathway amplifying the stress signals. Through these interactions ASK1 ensures the propagation of signals important for adaptive responses or programmed cell death.

Dysfunction in ASK1 activity associates with neurodegenerative diseases and cardiovascular disorders. In Alzheimer's disease for instance ASK1 contributes to neuronal apoptosis via its engagement with JNK signaling. In cardiovascular disorders ASK1 exacerbates heart failure by promoting cell death and inflammation. ASK1's interaction with proteins such as TNF receptor-associated factor 2 (TRAF2) highlights its role in these pathological conditions.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. Plays an important role in the cascades of cellular responses evoked by changes in the environment. Mediates signaling for determination of cell fate such as differentiation and survival. Plays a crucial role in the apoptosis signal transduction pathway through mitochondria-dependent caspase activation. MAP3K5/ASK1 is required for the innate immune response, which is essential for host defense against a wide range of pathogens. Mediates signal transduction of various stressors like oxidative stress as well as by receptor-mediated inflammatory signals, such as the tumor necrosis factor (TNF) or lipopolysaccharide (LPS). Once activated, acts as an upstream activator of the MKK/JNK signal transduction cascade and the p38 MAPK signal transduction cascade through the phosphorylation and activation of several MAP kinase kinases like MAP2K4/SEK1, MAP2K3/MKK3, MAP2K6/MKK6 and MAP2K7/MKK7. These MAP2Ks in turn activate p38 MAPKs and c-jun N-terminal kinases (JNKs). Both p38 MAPK and JNKs control the transcription factors activator protein-1 (AP-1).
See full target information MAP3K5 phospho S83
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Open medicine (Warsaw, Poland) 20:20251261 PubMed40918151

2025

CircASH1L-mediated tumor progression in triple-negative breast cancer: PI3K/AKT pathway mechanisms.

Applications

Unspecified application

Species

Unspecified reactive species

Haiyan Liu,Jiaming Wu,Jin Gong,Jing Zhu,Jie Liu,Xiaoqing Chen,Shaohua Qu,Jintao Chen,Zhisheng Zhou,Xulong Fan
View all publications
style
left-column
style
left-column
style
right-column

Complementary Products

Primary Antibodies

AB278570

APC Anti-p53 (phospho S15) antibody [P53S15-1C11]

Recombinant

Flow Cytometry - APC Anti-p53 (phospho S15) antibody [P53S15-1C11] (AB278570)

  • 0
  • 0
Primary Antibodies

AB108596

Anti-JAK2 antibody [EPR108(2)]

20ul selling size|RabMAb|Recombinant|KO Validated|Trial Size

Immunocytochemistry/ Immunofluorescence - Anti-JAK2 antibody [EPR108(2)] (AB108596)

  • 4
  • 3
Primary Antibodies

AB278695

FITC Anti-Src (phospho Y416) antibody [SrcY416-C4]

Recombinant

Flow Cytometry - FITC Anti-Src (phospho Y416) antibody [SrcY416-C4] (AB278695)

  • 0
  • 0
Primary Antibodies

AB278766

APC Anti-STAT5 (phospho Y694) antibody [Stat5Y694-G11]

Recombinant

Flow Cytometry - APC Anti-STAT5 (phospho Y694) antibody [Stat5Y694-G11] (AB278766)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com