JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB209750

PE Anti-ATP citrate lyase antibody [EP704Y]

5

(1 Review)

|

(1 Publication)

Rabbit Recombinant Monoclonal ATP citrate lyase antibody - conjugated to PE. Suitable for Flow Cyt (Intra), ICC/IF, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 1 publication.
1 Images
Flow Cytometry (Intracellular) - PE Anti-ATP citrate lyase antibody [EP704Y] (AB209750)
  • Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - PE Anti-ATP citrate lyase antibody [EP704Y] (AB209750)

Overlay histogram showing HeLa cells stained with ab209750 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 30 min at 22°C. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab209750, 1/5000 dilution) for 30 min at 22°C.

Isotype control antibody (black line) was rabbit IgG (monoclonal) Phycoerythrin (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

Acquisition of >5,000 events were collected using a 50mW Yellow-Green laser (561nm) and 586/15 bandpass filter.

  • Unconjugated

    Anti-ATP citrate lyase antibody [EP704Y]

  • Carrier free

    Anti-ATP citrate lyase antibody [EP704Y] - BSA and Azide free

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-ATP citrate lyase antibody [EP704Y]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-ATP citrate lyase antibody [EP704Y]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP704Y

Isotype

IgG

Conjugation

PE

Excitation/Emission

Ex: 480;565nm, Em: 578nm

Carrier free

No

Reacts with

Human, Human, Mouse, Rat

Applications

IHC-P, Flow Cyt (Intra), ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Alternative Products

Primary Antibodies

AB126129

Anti-ATP citrate lyase antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP citrate lyase antibody (AB126129)

  • 0
  • 0
Primary Antibodies

AB157098

Anti-ATP citrate lyase antibody

Western blot - Anti-ATP citrate lyase antibody (AB157098)

  • 0
  • 0
Primary Antibodies

AB235926

Anti-ATP citrate lyase antibody

Immunocytochemistry/ Immunofluorescence - Anti-ATP citrate lyase antibody (AB235926)

  • 0
  • 0
Primary Antibodies

AB53007

Anti-ATP citrate lyase (phospho T447 + S451) antibody [EP737Y]

RabMAb|Recombinant

Western blot - Anti-ATP citrate lyase (phospho T447 + S451) antibody [EP737Y] (AB53007)

  • 5
  • 1

Complementary Products

Assay Kits

AB83355

ATP Assay Kit (Colorimetric/Fluorometric)

Functional Studies - ATP Assay Kit (Colorimetric/Fluorometric) (AB83355)

  • 4
  • 5
Primary Antibodies

AB212122

PE Anti-IDH2 antibody [EPR7577]

RabMAb|Recombinant|KO Validated|Trial Size

Flow Cytometry (Intracellular) - PE Anti-IDH2 antibody [EPR7577] (AB212122)

  • 5
  • 1
Primary Antibodies

AB210451

PE Anti-ASS1 antibody [EPR12398]

RabMAb|Recombinant|KO Validated|Trial Size

Immunocytochemistry/ Immunofluorescence - PE Anti-ASS1 antibody [EPR12398] (AB210451)

  • 4
  • 1
Primary Antibodies

AB216385

Alexa Fluor® 594 Anti-ATP5A antibody [EPR13030(B)] - Mitochondrial Marker

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-ATP5A antibody [EPR13030(B)] - Mitochondrial Marker (AB216385)

  • 4
  • 1
Primary Antibodies

AB197536

Alexa Fluor® 488 Anti-Peroxiredoxin 2/PRP antibody [EPR5154]

RabMAb|Recombinant|Trial Size

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Peroxiredoxin 2/PRP antibody [EPR5154] (AB197536)

  • 0
  • 0
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/5000", "FlowCytIntra-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Rat": { "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Common marmoset": { "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
style
left-column

Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Do Not Freeze|Store in the dark
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ATP citrate lyase (ACL ACLY) is an enzyme responsible for converting citrate and coenzyme A into acetyl-CoA and oxaloacetate using ATP in the process. This reaction is key for lipid and cholesterol biosynthesis. ACLY's alternate name is ATP citrate (pro-S)-lyase and it has a molecular weight of approximately 120 kDa. The enzyme is expressed mainly in the cytoplasm with substantial amounts found in liver and adipose tissues.
Biological function summary

ATP citrate lyase plays an important role in lipid metabolism and energy production. It drives the conversion of citrate-derived acetyl-CoA a central metabolite in lipogenesis providing substrates for fatty acid and cholesterol synthesis. ACLY functions as a homotetramer complex to facilitate its enzymatic activity. This mechanism supports energy homeostasis linking carbohydrate metabolism with lipid biosynthesis.

Pathways

The enzyme ATP citrate lyase is instrumental in the citric acid cycle and fatty acid synthesis pathways. It is a central player in the cholesterol biosynthesis pathway which tightly connects to acetyl-CoA and citrate shuttle processes. In these pathways it interacts functionally with other enzymes such as acetyl-CoA carboxylase which further processes acetyl-CoA into malonyl-CoA serving as a precursor for fatty acid elongation.

ATP citrate lyase becomes significant in metabolic syndrome and cancer. Elevated expression and activity are linked to an increased risk of metabolic diseases including obesity and type 2 diabetes due to its role in excessive lipid accumulation. Moreover cancer cells often exhibit upregulated ACLY activity encouraging tumor growth by supplying acetyl-CoA for lipid biosynthesis. Its activity is intricately linked to proteins such as fatty acid synthase which also contribute to altered lipid profiles in these conditions.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Catalyzes the cleavage of citrate into oxaloacetate and acetyl-CoA, the latter serving as common substrate in multiple biochemical reactions in protein, carbohydrate and lipid metabolism.
See full target information ATP-citrate synthase

Alternative Names

ATP-citrate synthase, ATP-citrate (pro-S-)-lyase, Citrate cleavage enzyme, ACL, ACLY
style
left-column

Publications (1)

Recent publications for all applications. Explore the full listand refine your search

Arthritis research & therapy 27:173 PubMed40890810

2025

Transforming growth factor beta (TGF-β) induces type 1 interferon signalling in systemic sclerosis keratinocytes through the chloride intracellular channel 4 (CLIC4).

Applications

Unspecified application

Species

Unspecified reactive species

Christopher W Wasson,Sophie L Dibb,Begoña Caballero-Ruiz,Ifeoluwa E Bamigbola,Stefano Di Donato,Eva M Madourie-Clavane,Rebecca Wells,Vishal Kakkar,Enrico De Lorenzis,Jessica Bryon,Emma Derrett-Smith,Christopher P Denton,Yasser El-Sherbiny,Paul J Meakin,Rebecca L Ross,Francesco Del Galdo
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com