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Mouse Monoclonal C5a-R antibody - conjugated to PE. Suitable for Flow Cyt and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human C5AR1.

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Images

Flow Cytometry - PE Anti-C5a-R antibody [S5/1] (AB269287), expandable thumbnail
  • Flow Cytometry - PE Anti-C5a-R antibody [S5/1] (AB269287), expandable thumbnail

Key facts

Isotype
IgG2a
Host species
Mouse
Conjugation
PE
Excitation/Emission
Ex: 480;565nm, Em: 578nm
Storage buffer

pH: 7.4
Preservative: 0.1% Sodium azide
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • Synthetic Peptide within Human C5AR1. The exact immunogen used to generate this antibody is proprietary information. Database link P21730

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow Cyt
Human
Tested
Cow
Predicted
Ferret
Predicted
Rabbit
Predicted

Tested
Tested

Species
Human
Dilution info
10 µL for 106 Cells
Notes

Or 100 μL of whole blood.

Predicted
Predicted

Species
Rabbit, Cow, Ferret
Dilution info
-
Notes

-

Associated Products

Select an associated product type

3 products for Alternative Product

Target data

Function

Receptor for the chemotactic and inflammatory peptide anaphylatoxin C5a (PubMed:10636859, PubMed:15153520, PubMed:1847994, PubMed:29300009, PubMed:7622471, PubMed:8182049, PubMed:9553099). The ligand interacts with at least two sites on the receptor: a high-affinity site on the extracellular N-terminus, and a second site in the transmembrane region which activates downstream signaling events (PubMed:7622471, PubMed:8182049, PubMed:9553099). Receptor activation stimulates chemotaxis, granule enzyme release, intracellular calcium release and superoxide anion production (PubMed:10636859, PubMed:15153520).

Alternative names

Recommended products

Mouse Monoclonal C5a-R antibody - conjugated to PE. Suitable for Flow Cyt and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human C5AR1.

Key facts

Isotype
IgG2a
Conjugation
PE
Excitation/Emission
Ex: 480;565nm, Em: 578nm
Form
Liquid
Clonality
Monoclonal
Immunogen
  • Synthetic Peptide within Human C5AR1. The exact immunogen used to generate this antibody is proprietary information. Database link P21730
Clone number
S5/1
Purification technique
Size-exclusion chromatography
Specificity

ab269287 recognizes an extracellular epitope of CD88 protein.

Light chain type
kappa
Concentration
Loading...
Purification notes

The purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography.

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Store in the dark

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

C5a-R also known as CD88 or C5aR1 is a protein found primarily on the surface of immune cells like macrophages and neutrophils. Its mass is approximately 45 kDa. C5a-R functions as a G protein-coupled receptor (GPCR) and binds the complement component C5a a powerful inflammatory peptide. The receptor is expressed in various tissues but primarily in the immune system where it plays a significant role in immune responses and inflammatory processes.

Biological function summary

C5a-R functions as a mediator of inflammation and immune responses. It is a part of the complement system a complex of proteins involved in innate immunity. Upon binding to C5a this receptor initiates a signaling cascade that leads to the recruitment and activation of immune cells at sites of inflammation. It amplifies the inflammatory response leading to cell chemotaxis degranulation and the production of pro-inflammatory cytokines.

Pathways

C5a-R plays an important role in the complement and immune pathways. It interacts closely with the C5 protein which is a core component of the complement pathway. This receptor also connects to other immune system proteins such as the C3 and C4 proteins through the cascade. Together these interactions help defend against pathogenic infections and maintain immune homeostasis.

Associated diseases and disorders

C5a-R is associated with conditions like rheumatoid arthritis and sepsis. In rheumatoid arthritis the receptor's activation by C5a contributes to chronic inflammation and joint damage. During sepsis overactivation of C5a-R can lead to excessive inflammation and tissue damage. The receptor's involvement in these diseases is often linked with other proteins in the complement system such as C3a and C4a which also play roles in modulating inflammation and immune responses.

Product promise

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2 product images

  • Flow Cytometry - PE Anti-C5a-R antibody [S5/1] (ab269287), expandable thumbnail

    Flow Cytometry - PE Anti-C5a-R antibody [S5/1] (ab269287)

    Flow cytometric analysis of Human peripheral whole blood cells labelling C5a-R with ab269287 at 0.56 μg/ml.

  • Flow Cytometry - PE Anti-C5a-R antibody [S5/1] (ab269287), expandable thumbnail

    Flow Cytometry - PE Anti-C5a-R antibody [S5/1] (ab269287)

    Flow cytometric analysis of Human peripheral whole blood cells labelling C5a-R with ab269287 at 0.56 μg/ml.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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