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AB305486

PE Anti-CAD antibody [EP710Y]

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Rabbit Recombinant Monoclonal CAD antibody - conjugated to PE.

View Alternative Names

Multifunctional protein CAD, Carbamoyl phosphate synthetase 2-aspartate transcarbamylase-dihydroorotase, CAD

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP710Y

Isotype

IgG

Conjugation

PE

Excitation/Emission

Ex: 480;565nm, Em: 578nm

Carrier free

No

Applications

Target Binding Affinity, Antibody Labelling

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

ab40800 is specific to the N-terminus of CAD.

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Product details

This conjugated primary antibody is "made to order" and it is released using a quantitative quality control method that ensures binding affinity and labelling efficiency of the conjugate. Via leveraging the power of the Lightning-Link® conjugation technology, Abcam will deliver highly consistent recombinant conjugates in <2 weeks, giving you access to an ever growing portfolio of antibody-label combinations.

For suitable applications and species reactivity, please refer to the unconjugated version of this clone.

How are conjugated primary antibodies validated?
This conjugated primary antibody is released using a quantitative quality control method that evaluates binding affinity post-conjugation and efficiency of antibody labeling.
For suitable applications and species reactivity, please refer to the unconjugated version of this clone.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The CAD protein also known as carbamoyl-phosphate synthase 2/ aspartate transcarbamylase/ dihydroorotase serves an important mechanical role in pyrimidine biosynthesis. CAD is a multi-functional enzyme with a molecular mass of approximately 243 kDa and is expressed in the cytoplasm of many cell types. This complex protein organization enables it to catalyze the first three steps of de novo pyrimidine synthesis involving glutamine-dependent carbamoyl phosphate synthesis aspartate transcarbamylation and dihydroorotase activity. Such vital tasks establish CAD as an important player in the nucleotide biosynthesis landscape.
Biological function summary

The CAD enzyme facilitates the production of pyrimidine nucleotides that are necessary for DNA and RNA synthesis. CAD operates as a single-polypeptide chain trifunctional enzyme and is part of a larger protein complex involved in nucleic acid metabolism. Its enzymatic activities align closely to support cellular proliferation and differentiation processes that demand efficient nucleotide production. This link between CAD function and nucleotide availability underlines its importance in ensuring smooth cell cycle progression and maintenance.

Pathways

CAD integrates into the broader nucleotide metabolism and pyrimidine biosynthesis pathways. The CAD protein directly interacts with other key enzymes such as UMP synthase which furthers the conversion of its product dihydroorotate to UMP. OMP decarboxylase also acts downstream reflecting CAD's role in ensuring continuity and efficiency in pyrimidine synthesis. These interactions demonstrate CAD's central involvement in maintaining the nucleotide pool required for DNA synthesis and repair.

CAD mutations and dysregulations link to neurological disorders and cancer. Neurological conditions such as CAD deficiency can result from impaired pyrimidine synthesis affecting brain development and function. Additionally enhanced CAD activity associates with certain cancers where its upregulated pyrimidine production supports rapid tumor cell proliferation. In these disease contexts CAD's interaction with proteins involved in cellular proliferation pathways such as c-Myc in cancers highlights its impact on health and disease states.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Multifunctional protein that encodes the first 3 enzymatic activities of the de novo pyrimidine pathway : carbamoylphosphate synthetase (CPSase; EC 6.3.5.5), aspartate transcarbamylase (ATCase; EC 2.1.3.2) and dihydroorotase (DHOase; EC 3.5.2.3). The CPSase-function is accomplished in 2 steps, by a glutamine-dependent amidotransferase activity (GATase) that binds and cleaves glutamine to produce ammonia, followed by an ammonium-dependent carbamoyl phosphate synthetase, which reacts with the ammonia, hydrogencarbonate and ATP to form carbamoyl phosphate. The endogenously produced carbamoyl phosphate is sequestered and channeled to the ATCase active site. ATCase then catalyzes the formation of carbamoyl-L-aspartate from L-aspartate and carbamoyl phosphate. In the last step, DHOase catalyzes the cyclization of carbamoyl aspartate to dihydroorotate.
See full target information CAD protein
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