AB209577

PE Anti-Calreticulin antibody [EPR3924] - ER Marker

Name: PE conjugated Anti-Calreticulin antibody [EPR3924] - ER Marker (ab209577) | Abcam
Brand: Abcam Limited
SKU: AB209577
Price: 400 USD
Availability: InStock
Rating: 5 (1 reviews)

RabMAb
Recombinant
Lab Essentials
What is this?

(1 Review)

(11 Publications)

Anti-Calreticulin antibody [EPR3924] - ER Marker - PE conjugated (ab209577) is a rabbit recombinant monoclonal antibody detecting Calreticulin in Flow Cytometry (Intra), Flow Cytometry, ICC/IF. Suitable for Human.

- Biophysical QC for unrivalled batch-batch consistency

View Alternative Names

CRTC, CALR, Calreticulin, CRP55, Calregulin, Endoplasmic reticulum resident protein 60, HACBP, grp60, ERp60

2 Images

Immunocytochemistry/ Immunofluorescence - PE Anti-Calreticulin antibody [EPR3924] - ER Marker (AB209577)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - PE Anti-Calreticulin antibody [EPR3924] - ER Marker (AB209577)

ab209577 staining Calreticulin in HeLa cells. The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab209577 at 1/1000 dilution (pseudocolored in green) and ab195884, Rat monoclonal to Tubulin (Alexa Fluor^® 647), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Flow Cytometry (Intracellular) - PE Anti-Calreticulin antibody [EPR3924] - ER Marker (AB209577)

Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - PE Anti-Calreticulin antibody [EPR3924] - ER Marker (AB209577)

Overlay histogram showing HeLa cells stained with ab209577 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 90% methanol (-20°C) for 30 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab209577, 1/5000 dilution) for 30 min at 22°C.

Isotype control antibody (black line) was rabbit IgG (monoclonal) Phycoerythrin (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

Acquisition of >5,000 events were collected using a 20 mW Solid State Blue Laser (488nm) and 585/40 bandpass filter.

565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-Calreticulin antibody [EPR3924]
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-Calreticulin antibody [EPR3924] - ER Marker
421 Alexa Fluor® 405

Alexa Fluor® 405 Anti-Calreticulin antibody [EPR3924] - ER Marker
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-Calreticulin antibody [EPR3924] - ER Marker
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-Calreticulin antibody [EPR3924] - ER Marker
HRP

HRP Anti-Calreticulin antibody [EPR3924] - ER Marker
Unconjugated

Anti-Calreticulin antibody [EPR3924] - ER Marker
Carrier free

Anti-Calreticulin antibody [EPR3924] - BSA and Azide free (Capture)
Carrier free

Anti-Calreticulin antibody [EPR3924] - Low endotoxin, Azide free
Carrier free

Anti-Calreticulin antibody [EPR3924] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR3924

Isotype

IgG

Conjugation

Excitation/Emission

Ex: 480;565nm, Em: 578nm

Carrier free

Reacts with

Human

Applications

ICC/IF, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/1000", "ICCIF-species-notes": "<p>This product gave a positive signal in HeLa cells fixed with 100% methanol (5 min)</p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/5000", "FlowCytIntra-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Monkey": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

What is this antibody validated in?
PE Anti-Calreticulin antibody [EPR3924] - ER Marker (ab209577) is a rabbit recombinant monoclonal antibody and is validated for use in Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunocytochemistry/immunofluorescence (ICC/IF) in Human samples.

Related products
Antibody clone EPR3924 is also available pre-conjugated to a variety of labels for your convenience – Anti-Calreticulin PE [EPR3924] - ER Marker (ab209577).

Other related products
We have a range of other formats of antibody clone [EPR3924] also available for your convenience: ab92516, HRP - ab195511, Alexa Fluor® 488 - ab196158, Alexa Fluor® 647 - ab196159, PE - ab209577, Alexa Fluor® 405 - ab210431, Carrier free - ab211962, Carrier free - ab271865, Alexa Fluor® 555 - ab275117, Alexa Fluor® 594 - ab275343

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Do Not Freeze|Store in the dark

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Calcium-binding chaperone that promotes folding, oligomeric assembly and quality control in the endoplasmic reticulum (ER) via the calreticulin/calnexin cycle. This lectin interacts transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER (PubMed : 7876246). Interacts with the DNA-binding domain of NR3C1 and mediates its nuclear export (PubMed : 11149926). Involved in maternal gene expression regulation. May participate in oocyte maturation via the regulation of calcium homeostasis (By similarity). Present in the cortical granules of non-activated oocytes, is exocytosed during the cortical reaction in response to oocyte activation and might participate in the block to polyspermy (By similarity).

See full target information CALR

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Publications (11)

Recent publications for all applications. Explore the full list and refine your search

Journal for immunotherapy of cancer 13: PubMed40473272

2025

Enhancing antitumor immunity via ROS-ERS and pyroptosis-induced immunogenic cell death in multiple myeloma.

Applications

Unspecified application

Species

Unspecified reactive species

Liu Zhaoyun,Hao Wang,Chun Yang,Xianghong Zhao,Liu Hui,Jia Song,Kai Ding,Rong Fu

BMC complementary medicine and therapies 25:175 PubMed40369535

2025

A fermented Mistletoe (Viscum album L.) extract elicits markers characteristic for immunogenic cell death driven by endoplasmic reticulum stress in vitro.

Applications

Unspecified application

Species

Unspecified reactive species

Ulrike Weissenstein,Sibylle Tschumi,Bettina Leonhard,Stephan Baumgartner

Nature communications 16:3167 PubMed40175391

2025

Effective extracellular payload release and immunomodulatory interactions govern the therapeutic effect of trastuzumab deruxtecan (T-DXd).

Applications

Unspecified application

Species

Unspecified reactive species

Li-Chung Tsao,John S Wang,Xingru Ma,Sirajbir Sodhi,Joey V Ragusa,Bushangqing Liu,Jason McBane,Tao Wang,Junping Wei,Cong-Xiao Liu,Xiao Yang,Gangjun Lei,Ivan Spasojevic,Ping Fan,Timothy N Trotter,Michael Morse,Herbert Kim Lyerly,Zachary C Hartman

Molecular cancer therapeutics 24:118-130 PubMed39382078

2024

Tumor Integrin-Targeted Glucose Oxidase Enzyme Promotes ROS-Mediated Cell Death that Combines with Interferon Alpha Therapy for Tumor Control.

Applications

Unspecified application

Species

Unspecified reactive species

Jordan A Stinson,Allison Sheen,Brianna M Lax,Grace N Yang,Lauren Duhamel,Luciano Santollani,Elizabeth Fink,Joseph R Palmeri,Karl Dane Wittrup

Journal for immunotherapy of cancer 11: PubMed36759012

2023

Pharmacological tumor PDL1 depletion with chlorambucil treats ovarian cancer and melanoma: improves antitumor immunity and renders anti-PDL1-resistant tumors anti-PDL1-sensitive through NK cell effects.

Applications

Unspecified application

Species

Unspecified reactive species

Haiyan Bai,Alvaro S Padron,Yilun Deng,Yiji J Liao,Clare J Murray,Carlos Ontiveros,Suresh J Kari,Aravind Kancharla,Anand V R Kornepati,Myrna Garcia,Ryan Michael Reyes,Harshita B Gupta,Jose R Conejo-Garcia,Tyler Curiel

Nano research 15:2300-2314 PubMed36089987

2022

CD137 agonist potentiates the abscopal efficacy of nanoparticle-based photothermal therapy for melanoma.

Applications

Unspecified application

Species

Unspecified reactive species

Preethi Bala Balakrishnan,Debbie K Ledezma,Juliana Cano-Mejia,Jaclyn Andricovich,Erica Palmer,Vishal A Patel,Patricia S Latham,Eric S Yvon,Alejandro Villagra,Rohan Fernandes,Elizabeth E Sweeney

Advanced healthcare materials 11:e2201084 PubMed35943173

2022

Interstitial Photothermal Therapy Generates Durable Treatment Responses in Neuroblastoma.

Applications

Unspecified application

Species

Unspecified reactive species

Debbie K Ledezma,Preethi B Balakrishnan,Anshi Shukla,Jacob A Medina,Jie Chen,Emily Oakley,Catherine M Bollard,Gal Shafirstein,Mario Miscuglio,Rohan Fernandes

Journal of nanobiotechnology 20:252 PubMed35658868

2022

M1-derived extracellular vesicles enhance photodynamic therapy and promote immunological memory in preclinical models of colon cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Ruben V Huis In 't Veld,Pablo Lara,Martine J Jager,Roman I Koning,Ferry Ossendorp,Luis J Cruz

Scientific reports 11:12264 PubMed34112882

2021

Chemoradiation induces upregulation of immunogenic cell death-related molecules together with increased expression of PD-L1 and galectin-9 in gastric cancer.

Applications

Unspecified application

Species

Unspecified reactive species

S H Petersen,L F Kua,S Nakajima,W P Yong,K Kono

Biomolecules 10: PubMed32326356

2020

Sequential Interferon β-Cisplatin Treatment Enhances the Surface Exposure of Calreticulin in Cancer Cells via an Interferon Regulatory Factor 1-Dependent Manner.

Applications

Unspecified application

Species

Unspecified reactive species

Pei-Ming Yang,Yao-Yu Hsieh,Jia-Ling Du,Shih-Chieh Yen,Chien-Fu Hung

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

PE Anti-Calreticulin antibody [EPR3924] - ER Marker

Immunocytochemistry/ Immunofluorescence - PE Anti-Calreticulin antibody [EPR3924] - ER Marker (AB209577)

Flow Cytometry (Intracellular) - PE Anti-Calreticulin antibody [EPR3924] - ER Marker (AB209577)

Related conjugates and formulations (10)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (11)

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