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AB252099

PE Anti-cAMP Protein Kinase Catalytic subunit antibody [EP2102Y]

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Rabbit Recombinant Monoclonal cAMP Protein Kinase Catalytic subunit antibody - conjugated to PE. Suitable for Flow Cyt (Intra) and reacts with Human samples.

View Alternative Names

PKACA, PRKACA, cAMP-dependent protein kinase catalytic subunit alpha, PKA C-alpha

1 Images
Flow Cytometry (Intracellular) - PE Anti-cAMP Protein Kinase Catalytic subunit antibody [EP2102Y] (AB252099)
  • Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - PE Anti-cAMP Protein Kinase Catalytic subunit antibody [EP2102Y] (AB252099)

Overlay histogram showing MCF7 cells stained with ab252099 (red line). The cells were fixed with 80 % methanol (5 min) and then permeabilized with 0.1 % PBS-Triton X-100 for 15 min. The cells were incubated in 1x PBS containing 10 % normal goat serum to block non-specific protein-protein interaction followed by the antibody (ab252099) (1x 106 in 100μl at 0.5 μg/ml (1/1000)) for 30 min at 22°C.

Isotype control antibody (black line) was Rabbit IgG (monoclonal) Phycoerythrin (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

Acquisition of >5,000 events were collected using a 50 mW Yellow/Green laser (561nm) and 586/15 bandpass filter.

This antibody gave a positive signal in MCF7 cells fixed with 4 % formaldehyde (10 min) / permeabilized with 0.1 % PBS-Triton X-100 for 15 min used under the same conditions.

  • Unconjugated

    Anti-cAMP Protein Kinase Catalytic subunit antibody [EP2102Y]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-cAMP Protein Kinase Catalytic subunit antibody [EP2102Y]

  • Carrier free

    Anti-cAMP Protein Kinase Catalytic subunit antibody [EP2102Y] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP2102Y

Isotype

IgG

Conjugation

PE

Excitation/Emission

Ex: 480;565nm, Em: 578nm

Carrier free

No

Reacts with

Human

Applications

Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/1000", "FlowCytIntra-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: 98% PBS, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The cAMP Protein Kinase Catalytic subunit also known as protein kinase A (PKA) catalytic subunit plays an important role as an enzyme that phosphorylates various target proteins. This is an important component of the protein kinase A signaling pathway. The subunit has an approximate mass of 40 kDa and is widely expressed in multiple tissues across the body allowing it to affect numerous physiological functions. As a catalytic subunit of cAMP-dependent protein kinase it forms part of the larger enzyme complex that is activated by the binding of cAMP.
Biological function summary

The catalytic subunit of protein kinase A modulates cellular processes by transferring a phosphate group from ATP to specific serine/threonine residues on substrates. It is part of a holoenzyme complex composed of two regulatory and two catalytic subunits; when cAMP binds to the regulatory subunits it releases the catalytic subunits allowing them to execute their activity. This release and activation result in the regulation of glycogen sugar and lipid metabolism among others.

Pathways

The cAMP Protein Kinase integrates deeply into the cAMP signaling and MAPK/ERK pathways. These pathways regulate various processes like cell growth differentiation and apoptosis. The catalytic subunit acts downstream of cAMP generation by adenylyl cyclase interacting with other proteins such as CREB (cAMP response element-binding protein) which directly affects transcription and gene expression.

The cAMP Protein Kinase catalytic subunit has been linked to conditions including Carney complex and Cushing’s syndrome. Mutations affecting this subunit can lead to dysregulated cAMP signaling impacting proteins like regulatory subunit PRKAR1A that are important in controlling PKA activity. Conditions such as these often arise from aberrations in cAMP signaling pathways reflecting the extensive influence of the cAMP Protein Kinase on homeostasis and disease.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Phosphorylates a large number of substrates in the cytoplasm and the nucleus (PubMed : 15642694, PubMed : 15905176, PubMed : 16387847, PubMed : 17333334, PubMed : 17565987, PubMed : 17693412, PubMed : 18836454, PubMed : 19949837, PubMed : 20356841, PubMed : 21085490, PubMed : 21514275, PubMed : 21812984, PubMed : 31112131). Phosphorylates CDC25B, ABL1, NFKB1, CLDN3, PSMC5/RPT6, PJA2, RYR2, RORA, SOX9 and VASP (PubMed : 15642694, PubMed : 15905176, PubMed : 16387847, PubMed : 17333334, PubMed : 17565987, PubMed : 17693412, PubMed : 18836454, PubMed : 19949837, PubMed : 20356841, PubMed : 21085490, PubMed : 21514275, PubMed : 21812984). Regulates the abundance of compartmentalized pools of its regulatory subunits through phosphorylation of PJA2 which binds and ubiquitinates these subunits, leading to their subsequent proteolysis (PubMed : 21423175). RORA is activated by phosphorylation (PubMed : 21514275). Required for glucose-mediated adipogenic differentiation increase and osteogenic differentiation inhibition from osteoblasts (PubMed : 19949837). Involved in chondrogenesis by mediating phosphorylation of SOX9 (By similarity). Involved in the regulation of platelets in response to thrombin and collagen; maintains circulating platelets in a resting state by phosphorylating proteins in numerous platelet inhibitory pathways when in complex with NF-kappa-B (NFKB1 and NFKB2) and I-kappa-B-alpha (NFKBIA), but thrombin and collagen disrupt these complexes and free active PRKACA stimulates platelets and leads to platelet aggregation by phosphorylating VASP (PubMed : 15642694, PubMed : 20356841). Prevents the antiproliferative and anti-invasive effects of alpha-difluoromethylornithine in breast cancer cells when activated (PubMed : 17333334). RYR2 channel activity is potentiated by phosphorylation in presence of luminal Ca(2+), leading to reduced amplitude and increased frequency of store overload-induced Ca(2+) release (SOICR) characterized by an increased rate of Ca(2+) release and propagation velocity of spontaneous Ca(2+) waves, despite reduced wave amplitude and resting cytosolic Ca(2+) (PubMed : 17693412). PSMC5/RPT6 activation by phosphorylation stimulates proteasome (PubMed : 17565987). Negatively regulates tight junctions (TJs) in ovarian cancer cells via CLDN3 phosphorylation (PubMed : 15905176). NFKB1 phosphorylation promotes NF-kappa-B p50-p50 DNA binding (PubMed : 15642694). Required for phosphorylation of GLI transcription factors which inhibits them and prevents transcriptional activation of Hedgehog signaling pathway target genes (By similarity). GLI transcription factor phosphorylation is inhibited by interaction of PRKACA with SMO which sequesters PRKACA at the cell membrane (By similarity). Involved in embryonic development by down-regulating the Hedgehog (Hh) signaling pathway that determines embryo pattern formation and morphogenesis most probably through the regulation of OFD1 in ciliogenesis (PubMed : 33934390). Prevents meiosis resumption in prophase-arrested oocytes via CDC25B inactivation by phosphorylation (By similarity). May also regulate rapid eye movement (REM) sleep in the pedunculopontine tegmental (PPT) (By similarity). Phosphorylates APOBEC3G and AICDA (PubMed : 16387847, PubMed : 18836454). Phosphorylates HSF1; this phosphorylation promotes HSF1 nuclear localization and transcriptional activity upon heat shock (PubMed : 21085490). Acts as a negative regulator of mTORC1 by mediating phosphorylation of RPTOR (PubMed : 31112131).. Isoform 2. Phosphorylates and activates ABL1 in sperm flagellum to promote spermatozoa capacitation.
See full target information PRKACA
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Associated Products

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Alternative Version
Primary Antibodies

AB252098

Alexa Fluor® 647 Anti-cAMP Protein Kinase Catalytic subunit antibody [EP2102Y]

primary-antibodies

alexa-fluor-647-camp-protein-kinase-catalytic-subunit-antibody-ep2102y-ab252098

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Alternative Version
Primary Antibodies

AB235385

Anti-cAMP Protein Kinase Catalytic subunit antibody [EP2102Y] - BSA and Azide free

primary-antibodies

camp-protein-kinase-catalytic-subunit-antibody-ep2102y-bsa-and-azide-free-ab235385

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(0 reviews)

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