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AB322129

PE Anti-Cardiac Troponin T antibody [EPR20266]

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Rabbit Recombinant Monoclonal Cardiac Troponin T antibody - conjugated to PE. Suitable for ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Mouse, Rat, Human samples.

View Alternative Names

TnTc, Cardiac muscle troponin T, cTnT, TNNT2

4 Images
Flow Cytometry (Intracellular) - PE Anti-Cardiac Troponin T antibody [EPR20266] (AB322129)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - PE Anti-Cardiac Troponin T antibody [EPR20266] (AB322129)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized L6 (rat skeletal muscle myoblast) cells labelling Troponin T with ab322129 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Phycoerythrin) (ab209478) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Immunocytochemistry/ Immunofluorescence - PE Anti-Cardiac Troponin T antibody [EPR20266] (AB322129)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - PE Anti-Cardiac Troponin T antibody [EPR20266] (AB322129)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized L6 (rat skeletal muscle myoblast) cells labelling Troponin T with ab322129 at 1/50 (10.0 ug/ml) dilution (Green).

Confocal image showing cytoplasmic staining in L6 cells (shown in magenta). The counterstain was observed in green. Nuclear DNA was labeled with DAPI (shown in blue). Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

ab195887 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 488) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

Flow Cytometry (Intracellular) - PE Anti-Cardiac Troponin T antibody [EPR20266] (AB322129)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - PE Anti-Cardiac Troponin T antibody [EPR20266] (AB322129)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HL-1 (mouse atrial muscle cell) cells labelling Troponin T with ab322129 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Phycoerythrin) (ab209478) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Immunocytochemistry/ Immunofluorescence - PE Anti-Cardiac Troponin T antibody [EPR20266] (AB322129)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - PE Anti-Cardiac Troponin T antibody [EPR20266] (AB322129)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HL-1 (mouse atrial muscle cell) cells labelling Troponin T with ab322129 at 1/50 (10.0 ug/ml) dilution (Green).

Confocal image showing cytoplasmic staining in HL-1 cells (shown in magenta). The counterstain was observed in green. Nuclear DNA was labeled with DAPI (shown in blue). Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

ab195887 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 488) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

  • Carrier free

    Anti-Cardiac Troponin T antibody [EPR20266] - BSA and Azide free

  • Unconjugated

    Anti-Cardiac Troponin T antibody [EPR20266]

  • 660 APC

    APC Anti-Cardiac Troponin T antibody [EPR20266]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-Cardiac Troponin T antibody [EPR20266]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-Cardiac Troponin T antibody [EPR20266]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR20266

Isotype

IgG

Conjugation

PE

Excitation/Emission

Ex: 480;565nm, Em: 578nm

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

IHC-P, Flow Cyt (Intra), ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Complementary Products

Assay Kits

AB223860

Human Cardiac Troponin T ELISA Kit

Recombinant|SimpleStep

ELISA - Human Cardiac Troponin T ELISA Kit (AB223860)

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Assay Kits

AB223860

Human Cardiac Troponin T ELISA Kit

Recombinant|SimpleStep

ELISA - Human Cardiac Troponin T ELISA Kit (AB223860)

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Primary Antibodies

AB15580

Anti-Ki67 antibody

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody (AB15580)

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Proteins & Peptides

AB316587

Recombinant Human Cardiac Troponin T Protein Standard (His tag)

Sandwich ELISA - Recombinant Human Cardiac Troponin T Protein Standard (His tag) (AB316587)

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Primary Antibodies

AB181604

Anti-HNF-4-alpha antibody [EPR16885] - ChIP Grade

20ul selling size|RabMAb|Advanced Validation|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-4-alpha antibody [EPR16885] - ChIP Grade (AB181604)

  • 5
  • 4
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/500", "FlowCytIntra-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Rat": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/500", "FlowCytIntra-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.
See full target information TNNT2
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com