JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB224917

PE Anti-CBL antibody [YE323] - C-terminal

Be the first to review this product! Submit a review

|

(1 Publication)

Rabbit Recombinant Monoclonal CBL antibody - conjugated to PE. C-terminal. Suitable for Flow Cyt (Intra), ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

View Alternative Names

CBL2, RNF55, CBL, E3 ubiquitin-protein ligase CBL, Casitas B-lineage lymphoma proto-oncogene, Proto-oncogene c-Cbl, RING finger protein 55, RING-type E3 ubiquitin transferase CBL, Signal transduction protein CBL

1 Images
Flow Cytometry (Intracellular) - PE Anti-CBL antibody [YE323] - C-terminal (AB224917)
  • Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - PE Anti-CBL antibody [YE323] - C-terminal (AB224917)

Overlay histogram showing Jurkat (Human T cell leukemia cell line from peripheral blood) cells stained with ab224917 (red line).

The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab224917, 1/5000 dilution) for 30 min at 22°C.

Isotype control antibody (black line) was Rabbit IgG (monoclonal) Phycoerythrin (ab209478) used at the same concentration and conditions as the primary antibody. Unlabeled sample (blue line) was also used as a control.

Acquisition of >5,000 events were collected using a 50 mW Yellow/Green laser (561nm) and 586/15 bandpass filter.

This antibody gave a positive signal in Jurkat cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Triton X-100 for 15 min used under the same conditions.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

YE323

Isotype

IgG

Conjugation

PE

Excitation/Emission

Ex: 480;565nm, Em: 578nm

Carrier free

No

Reacts with

Human, Human, Mouse, Rat

Applications

Flow Cyt (Intra), ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Epitope

ab224917 reacts with an epitope located in the C terminal region of CBL.

style
left-column

Alternative Products

Primary Antibodies

AB131345

Anti-CBL (phospho Y700) antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CBL (phospho Y700) antibody (AB131345)

  • 0
  • 0
Primary Antibodies

AB108364

Anti-CBL (phospho S669) antibody [EPR2226(2)]

20ul selling size|RabMAb|Recombinant

Western blot - Anti-CBL (phospho S669) antibody [EPR2226(2)] (AB108364)

  • 3
  • 1
Primary Antibodies

AB32446

Anti-CBL (phospho Y774) antibody [E160]

20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CBL (phospho Y774) antibody [E160] (AB32446)

  • 0
  • 0
Primary Antibodies

AB76536

Anti-CBL (phospho Y674) antibody [EPR2227]

20ul selling size|RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-CBL (phospho Y674) antibody [EPR2227] (AB76536)

  • 0
  • 0
Primary Antibodies

AB76002

Anti-CBL (phospho Y700) antibody [EP2225Y]

20ul selling size|RabMAb

Flow Cytometry (Intracellular) - Anti-CBL (phospho Y700) antibody [EP2225Y] (AB76002)

  • 0
  • 0
Primary Antibodies

AB52855

Anti-CBL (phospho Y731) antibody [EP973Y]

20ul selling size|RabMAb

Western blot - Anti-CBL (phospho Y731) antibody [EP973Y] (AB52855)

  • 0
  • 0

Complementary Products

Proteins & Peptides

AB280943

Recombinant human PD-L1 protein (Active)

Premium Bioactive

Sandwich ELISA - Recombinant human PD-L1 protein (Active) (AB280943)

  • 0
  • 0
Primary Antibodies

AB16699

Anti-CD5 antibody [SP19]

BOND RX™ Validated|RabMAb|Recombinant|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD5 antibody [SP19] (AB16699)

  • 0
  • 0
Primary Antibodies

AB174831

Anti-MMAB antibody [EPR8695]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-MMAB antibody [EPR8695] (AB174831)

  • 0
  • 0
Primary Antibodies

AB9485

Anti-GAPDH antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody - Loading Control (AB9485)

  • 5
  • 88
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/5000", "FlowCytIntra-species-notes": "<p>The cellular localisation of this product has been verified in ICC/IF.</p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Rat": { "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Chicken": { "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
style
left-column

Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Store in the dark
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The CBL protein also known as C-cbl or Casitas B-lineage lymphoma proto-oncogene serves as an E3 ubiquitin-protein ligase. This protein with a molecular mass of approximately 120-130 kDa plays a critical role in the regulation of receptor tyrosine kinases. It facilitates the polyubiquitination of activated receptor proteins targeting them for degradation in the proteasome. CBL is predominantly expressed in various tissues including the hematopoietic system. It is a significant component in the control of cell signaling and helps maintain cellular homeostasis.
Biological function summary

The CBL protein acts as a negative regulator for several signaling pathways. It integrates into signaling complexes and modulates the activation of pathways by interacting with other proteins. CBL does not act alone; it commonly forms complexes with proteins such as Grb2 and p85 influencing downstream signaling influence in pathways. This regulation prevents excessive activation of signals that can lead to cellular proliferation or differentiation abnormally.

Pathways

CBL integrates into the ubiquitin-proteasome system and the Ras signaling pathway. Within these pathways CBL works to modulate cell surface receptors ensuring that signaling threshold is not surpassed. The importance of CBL in the Ras signaling pathway is highlighted by its interactions with proteins like Ras-GAP and SHP2. Through these interactions CBL controls the rate of signal transduction affecting cellular responses such as growth survival and motility.

CBL involvement has been linked to several forms of cancer and immune deficiencies. Mutations or dysregulation of the CBL gene can lead to myelodysplastic syndromes and acute myeloid leukemia. In these conditions the interaction between CBL and other proteins such as JAK2 becomes dysregulated driving abnormal cell growth and survival. Furthermore CBL’s failure to properly degrade tyrosine kinase receptors can contribute to enhanced signaling fostering oncogenic processes.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

E3 ubiquitin-protein ligase that acts as a negative regulator of many signaling pathways by mediating ubiquitination of cell surface receptors (PubMed : 10514377, PubMed : 11896602, PubMed : 14661060, PubMed : 14739300, PubMed : 15190072, PubMed : 17509076, PubMed : 18374639, PubMed : 19689429, PubMed : 21596750, PubMed : 28381567, PubMed : 40101708). Accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and then transfers it to substrates promoting their degradation by the proteasome (PubMed : 10514377, PubMed : 14661060, PubMed : 14739300, PubMed : 17094949, PubMed : 17509076, PubMed : 17974561). Recognizes activated receptor tyrosine kinases, including KIT, FLT1, FGFR1, FGFR2, PDGFRA, PDGFRB, CSF1R, EPHA8 and KDR and mediates their ubiquitination to terminate signaling (PubMed : 15190072, PubMed : 18374639, PubMed : 21596750). Recognizes membrane-bound HCK, SRC and other kinases of the SRC family and mediates their ubiquitination and degradation (PubMed : 11896602). Ubiquitinates EGFR and SPRY2 (PubMed : 17094949, PubMed : 17974561). Involved in LAG3-mediated inhibition of TCR signaling : following ligand-binding to LAG3, catalyzes 'Lys-63'-linked ubiquitination of LAG3, unleashing the LAG3 C-terminus from the membrane, and initiating a signaling that prevents TCR activation (PubMed : 40101708). Ubiquitinates NECTIN1 following association between NECTIN1 and herpes simplex virus 1/HHV-1 envelope glycoprotein D, leading to NECTIN1 removal from cell surface (PubMed : 28381567). Participates in signal transduction in hematopoietic cells. Plays an important role in the regulation of osteoblast differentiation and apoptosis (PubMed : 15190072, PubMed : 18374639). Essential for osteoclastic bone resorption (PubMed : 14739300). The 'Tyr-731' phosphorylated form induces the activation and recruitment of phosphatidylinositol 3-kinase to the cell membrane in a signaling pathway that is critical for osteoclast function (PubMed : 14739300). In association with CBLB, required for proper feedback inhibition of ciliary platelet-derived growth factor receptor-alpha (PDGFRA) signaling pathway via ubiquitination and internalization of PDGFRA (By similarity).
See full target information CBL
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Immunity 55:2369-2385.e10 PubMed36370712

2022

The transcription factor IRF2 drives interferon-mediated CD8 T cell exhaustion to restrict anti-tumor immunity.

Applications

Unspecified application

Species

Unspecified reactive species

Sabelo Lukhele,Diala Abd Rabbo,Mengdi Guo,Jian Shen,Heidi J Elsaesser,Rene Quevedo,Madeleine Carew,Ramy Gadalla,Laura M Snell,Lawanya Mahesh,M Teresa Ciudad,Bryan E Snow,Annick You-Ten,Jillian Haight,Andrew Wakeham,Pamela S Ohashi,Tak W Mak,Weiguo Cui,Tracy L McGaha,David G Brooks
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com