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AB245899

PE Anti-CCR2 antibody [EPR20844]

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(1 Publication)

Rabbit Recombinant Monoclonal CCR2 antibody - conjugated to PE. Suitable for Flow Cyt and reacts with Mouse samples. Cited in 1 publication.

View Alternative Names

CD192, Cmkbr2, Ccr2, C-C chemokine receptor type 2, C-C CKR-2, CC-CKR-2, CCR-2, CCR2, JE/FIC receptor, MCP-1 receptor

1 Images
Flow Cytometry - PE Anti-CCR2 antibody [EPR20844] (AB245899)
  • Flow Cyt

Unknown

Flow Cytometry - PE Anti-CCR2 antibody [EPR20844] (AB245899)

C57BL/6 mouse splenocytes stained with ab245899 (right) or Rabbit IgG (monoclonal) Phycoerythrin (Isotype control (ab209478)) (left). C57BL/6 Mouse splenocytes were incubated for 30 min on ice in 1x PBS containing 10 μg/ml anti CD16/32 antibody and 10% normal goat serum to block FC receptors and non-specific protein-protein interactions followed by the antibody (ab245899) or Rabbit IgG (monoclonal) Phycoerythrin (Isotype control (ab209478) (1x 106 cells in 100 μl at 1 μg/ml (1/500)) for 30 min on ice. The cells were simultaneously stained with Ly6G/GR1 PerCP conjugated antibody (ab236728).

Acquisition of >30,000 events were collected using a 50 mW Yellow/Green laser (561 nm) and 586/15 bandpass filter.

Events were gated on viable lymphocytes.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR20844

Isotype

IgG

Conjugation

PE

Excitation/Emission

Ex: 480;565nm, Em: 578nm

Carrier free

No

Reacts with

Mouse

Applications

Flow Cyt

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/500", "FlowCyt-species-notes": "<p>The cellular localisation of this product has been verified in ICC/IF.</p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CCR2 also known as C-C chemokine receptor type 2 is a protein involved in immune cell trafficking. It is a member of the G-protein-coupled receptor family and has a molecular mass of approximately 43 kDa. CCR2 is primarily expressed on monocytes dendritic cells and certain subsets of T cells. It functions as a receptor for monocyte chemoattractant proteins (MCPs) with MCP-1 (CCL2) being the most well-known ligand. The interaction between CCR2 and its ligands directs the movement of these immune cells to sites of inflammation or tissue injury.
Biological function summary

CCR2 plays an important role in mediating leukocyte migration. It acts in the immune system to guide monocytes from the bloodstream into tissues contributing to immune surveillance and response. CCR2 operates not as part of a larger receptor complex but it does interact closely with other chemokine receptors which may influence its signaling. The receptor's activity has critical implications for inflammatory processes and lies at the heart of many immune responses.

Pathways

CCR2 is integrally involved in the chemokine signaling pathway and the inflammatory response pathway. Its function in these pathways highlights its role in modulating immune cell infiltration during immune challenges. The receptor also interfaces with other important signaling proteins such as CCR5 which like CCR2 is another chemokine receptor involved in mediating immune cell movement. These interactions overlap and complement each other offering nuanced regulation of immune cell dynamics.

CCR2 has connections to conditions such as atherosclerosis and rheumatoid arthritis. In atherosclerosis the receptor's involvement in monocyte recruitment to the arterial wall is an important step in plaque formation. Its role in rheumatoid arthritis centers on the promotion of leukocyte infiltration into the joint tissues contributing to inflammation and joint damage. CCR2's connection to such disorders often aligns with a similar role played by other chemokine receptors like CCR5 highlighting its relevance in inflammation-related pathologies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Key functional receptor for CCL2 but can also bind CCL7 and CCL12 chemokines (PubMed : 8631787, PubMed : 8662823, PubMed : 8996246). Its binding with CCL2 on monocytes and macrophages mediates chemotaxis and migration induction through the activation of the PI3K cascade, the small G protein Rac and lamellipodium protrusion (By similarity). Also acts as a receptor for the beta-defensin DEFB106A/DEFB106B (By similarity). Regulates the expression of T-cell inflammatory cytokines and T-cell differentiation, promoting the differentiation of T-cells into T-helper 17 cells (Th17) during inflammation (PubMed : 28507030). Facilitates the export of mature thymocytes by enhancing directional movement of thymocytes to sphingosine-1-phosphate stimulation and up-regulation of S1P1R expression; signals through the JAK-STAT pathway to regulate FOXO1 activity leading to an increased expression of S1P1R (PubMed : 29930553). Plays an important role in mediating peripheral nerve injury-induced neuropathic pain (PubMed : 29993042). Increases NMDA-mediated synaptic transmission in both dopamine D1 and D2 receptor-containing neurons, which may be caused by MAPK/ERK-dependent phosphorylation of GRIN2B/NMDAR2B (PubMed : 29993042). Mediates the recruitment of macrophages and monocytes to the injury site following brain injury (PubMed : 24806994, PubMed : 29632244).
See full target information Ccr2
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in medicine 9:897800 PubMed35911406

2022

Establishment of the reproducible branch retinal artery occlusion mouse model and intravital longitudinal imaging of the retinal CX3CR1-GFP cells after spontaneous arterial recanalization.

Applications

Unspecified application

Species

Unspecified reactive species

Jehwi Jeon,Sang-Hoon Kim,Eunji Kong,Soo Jin Kim,Jee Myung Yang,Joo Yong Lee,Junyeop Lee,You-Me Kim,Pilhan Kim
View all publications
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Product promise

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