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Rat Monoclonal CCR7 antibody - conjugated to PE. Suitable for Flow Cyt and reacts with Mouse samples. Cited in 2 publications. Immunogen corresponding to Cell preparation containing Ccr7 protein.

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Images

Flow Cytometry - PE Anti-CCR7 antibody [4B12] (AB95669), expandable thumbnail

Publications

Key facts

Isotype

IgG2a

Host species

Rat

Conjugation

PE

Excitation/Emission

Ex: 480;565nm, Em: 578nm

Storage buffer

pH: 7.2
Preservative: 0.09% Sodium azide
Constituents: PBS, 0.87% Sodium chloride

Form

Liquid

Clonality

Monoclonal

Immunogen

  • Cell preparation containing Ccr7 protein. The exact immunogen used to generate this antibody is proprietary information. Database link P47774

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow Cyt
Mouse
Tested

Tested
Tested

Species

Mouse

Dilution info

1-5 µg for 106 Cells

Notes

ab134674 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

Associated Products

Select an associated product type

1 product for Alternative Version

1 product for Alternative Product

Target data

Function

Receptor for the MIP-3-beta chemokine. Probable mediator of EBV effects on B-lymphocytes or of normal lymphocyte functions.

Alternative names

Recommended products

Rat Monoclonal CCR7 antibody - conjugated to PE. Suitable for Flow Cyt and reacts with Mouse samples. Cited in 2 publications. Immunogen corresponding to Cell preparation containing Ccr7 protein.

Key facts

Isotype

IgG2a

Conjugation

PE

Excitation/Emission

Ex: 480;565nm, Em: 578nm

Form

Liquid

Clonality

Monoclonal

Immunogen
  • Cell preparation containing Ccr7 protein. The exact immunogen used to generate this antibody is proprietary information. Database link P47774
Clone number

4B12

Purification technique

Affinity purification Protein G

Specificity

ab95669 stains Mouse CCR7-GFP fusion protein-transfected RBL cells

Light chain type

kappa

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Notes

Excitation: 488 nm Emission: 575 nm.

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Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

CCR7 also known as CD197 is a chemokine receptor with a molecular mass of approximately 43-48 kDa. The receptor is known to be expressed predominantly in lymphoid tissues such as the spleen and lymph nodes. Additionally CCR7 is found on various immune cells like T cells and dendritic cells. Researchers study CCR7 using different methods such as CCR7-PE and CCR7-APC antibody staining in flow cytometry to examine its expression on immune cells in various conditions.

Biological function summary

CCR7 plays an important role in directing the migration and homeostasis of T cells and dendritic cells. It is not part of a larger protein complex but interacts functionally with specific signaling enzymes upon activation. CCR7 is important for the function of the immune system aiding in the development of adaptive immunity by controlling lymphocyte movement and positioning in lymphoid tissues. This receptor assists in guiding cells to specific lymphoid organs where immune responses are initiated and maintained.

Pathways

CCR7 is significantly involved in the CCL19/CCL21 chemokine signaling pathways which help direct immune cell positioning. CCR7 interacts with these chemokines to regulate the trafficking of cells to lymphoid tissues. Additionally it works in tandem with other receptors like CCR5 in the regulation of immune cell mobilization. These pathways are essential in coordinating the immune response during infection and inflammation.

Associated diseases and disorders

CCR7 is connected with cancer metastasis and chronic inflammatory diseases. Its role in cell migration makes it relevant in the metastasis of certain cancers where cells misregulate their movement and spread from the original tumor site. Additionally CCR7's involvement in chronic inflammatory diseases highlights its connection to rheumatoid arthritis where it may interact with other proteins such as CCL19 and CCL21 contributing to disease progression. Understanding these interactions helps develop potential therapeutic strategies targeting CCR7 in such diseases.

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1 product image

  • Flow Cytometry - PE Anti-CCR7 antibody [4B12] (ab95669), expandable thumbnail

    Flow Cytometry - PE Anti-CCR7 antibody [4B12] (ab95669)

    Staining of C57BL/6 splenocytes with Anti-Mouse CD4 FITC and 0.5 µg of ab95669 with staining carried out at 4 °C (left) or 37 °C (right). Cells in the lymphocyte gate were used for analysis

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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