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AB95763

PE Anti-CD103 antibody [OX62]

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(4 Publications)

Mouse Monoclonal ITGAE antibody - conjugated to PE. Suitable for Flow Cyt and reacts with Rat samples. Cited in 4 publications. Immunogen corresponding to Cell preparation containing ITGAE protein.
1 Images
Flow Cytometry - PE Anti-CD103 antibody [OX62] (AB95763)
  • Flow Cyt

Unknown

Flow Cytometry - PE Anti-CD103 antibody [OX62] (AB95763)

Flow cytometry staining of rat splenocytes with Anti-Rat CD4 FITC and 0.125 µg of Mouse IgG1 kappa Isotype Control PE (left) or 0.125 µg of ab95763 (right). Cells in the lymphocyte gate were used for analysis and events are displayed after gating out CD3+ T cells and CD45RB+ cells and gating on the remaining cells, which are MHC Class II+.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

OX62

Isotype

IgG1

Conjugation

PE

Excitation/Emission

Ex: 480;565nm, Em: 578nm

Carrier free

No

Reacts with

Rat

Applications

Flow Cyt

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Rat": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "0.125-0.25 µg for 10^5 Cells", "FlowCyt-species-notes": "<p>Cell number should be determined empirically but can range from 105 to 108 cells/test (based on a test volume of 100 μl).</p><p><a href='/en-us/products/primary-antibodies/pe-mouse-igg1-b11-6-isotype-control-ab91357'>ab91357</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Storage buffer
pH: 7.2 Preservative: 0.09% Sodium azide
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD103 also known as ITGAE or integrin alpha E is a protein subunit that pairs with integrin beta 7 (ItgB7) to form a complete heterodimer known as the αEβ7 integrin complex. This protein typically weighs around 150 kDa. CD103 is mainly expressed on the surface of certain T lymphocytes such as CD8+ T cells in mucosal tissues and also in a subset of dendritic cells. Through its mechanical function CD103 mediates cell adhesion by specifically binding to E-cadherin on epithelial cells. This interaction facilitates the retention and positioning of lymphocytes in epithelial tissues.
Biological function summary

CD103 is essential for establishing and maintaining interactions between immune cells and epithelial cells. By incorporating into the αEβ7 integrin complex it plays an important role in the localization of intraepithelial lymphocytes and dendritic cells in the gut mucosa and skin contributing to immune surveillance and protection of these barriers. CD103 helps to control lymphocyte activation and the migration process offering a critical surface marker for identifying tissue-resident memory T cells (Trm cells) and discerning their functional potential.

Pathways

CD103 functions prominently in the immunological environment by participating in pathways that regulate lymphocyte adherence and migration. Two significant pathways it fits into include the T-cell homing and retention pathways and the antigen presentation pathway. Through these pathways CD103 interacts with molecules such as E-cadherin and impacts the movement of T cells to epithelial sites influencing tissue-specific immune responses alongside related proteins like integrin beta 7 which forms a part of the same integrin complex.

CD103 has a significant connection with inflammatory and autoimmune conditions particularly in the context of inflammatory bowel disease and certain types of cancer such as colorectal cancer. Its association with E-cadherin through the integrin complex affects cellular behavior in these diseases contributing to either barrier integrity or breakdown and abnormal immune cell infiltration. Additionally in the disease context CD103 can influence interactions with proteins that regulate immune responses and tumor surveillance highlighting its role in disease progression and therapeutic potential.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information ITGAE
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

The Journal of surgical research 227:17-27 PubMed29804850

2018

Adipose-derived mesenchymal stem cells attenuate rejection in a rat lung transplantation model.

Applications

IHC-P

Species

Unspecified reactive species

Hironosuke Watanabe,Tomoshi Tsuchiya,Koichiro Shimoyama,Akira Shimizu,Sadanori Akita,Hiroshi Yukawa,Yoshinobu Baba,Takeshi Nagayasu

Journal of immunology (Baltimore, Md. : 1950) 172:7485-94 PubMed15187127

2004

Rat plasmacytoid dendritic cells are an abundant subset of MHC class II+ CD4+CD11b-OX62- and type I IFN-producing cells that exhibit selective expression of Toll-like receptors 7 and 9 and strong responsiveness to CpG.

Applications

Unspecified application

Species

Unspecified reactive species

Francois-Xavier Hubert,Cécile Voisine,Cédric Louvet,Michèle Heslan,Régis Josien

Journal of immunology (Baltimore, Md. : 1950) 169:2284-91 PubMed12193693

2002

Two phenotypically distinct subsets of spleen dendritic cells in rats exhibit different cytokine production and T cell stimulatory activity.

Applications

Unspecified application

Species

Unspecified reactive species

Cécile Voisine,Francois-Xavier Hubert,Benjamin Trinité,Michèle Heslan,Régis Josien

The Journal of experimental medicine 175:1457-65 PubMed1588275

1992

The MRC OX-62 antigen: a useful marker in the purification of rat veiled cells with the biochemical properties of an integrin.

Applications

Flow Cyt

Species

Unspecified reactive species

M Brenan,M Puklavec
View all publications
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