AB93567

PE Anti-CD105 antibody [MJ7/18]

Name: PE Anti-CD105 antibody [MJ7/18] (ab93567) | Abcam
Brand: Abcam Limited
SKU: AB93567
Price: 250 USD
Availability: InStock

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(8 Publications)

Rat Monoclonal CD105 antibody - conjugated to PE. Suitable for Flow Cyt and reacts with Mouse samples. Cited in 8 publications. Immunogen corresponding to Tissue preparation containing Eng protein.

View Alternative Names

CD105, Edg, Eng, Endoglin, Cell surface MJ7/18 antigen

1 Images

Flow Cytometry - PE Anti-CD105 antibody [MJ7/18] (AB93567)

Flow Cyt

Unknown

Flow Cytometry - PE Anti-CD105 antibody [MJ7/18] (AB93567)

Staining of bEnd.3 (Mouse brain endothelioma cell line) whole cell lysate with 0.25 μg of PE Rat IgG2a Isotype Control (open histogram) or 0.25 μg of ab93567 (colored histogram). Total viable cells were used for analysis.

Unconjugated

Anti-CD105 antibody [MJ7/18]
Biotin

Biotin Anti-CD105 antibody [MJ7/18]
Carrier free

Anti-CD105 antibody [MJ7/18] - BSA and Azide free

Key facts

Host species

Rat

Clonality

Monoclonal

Clone number

MJ7/18

Isotype

IgG2a

Light chain type

kappa

Conjugation

Excitation/Emission

Ex: 480;565nm, Em: 578nm

Carrier free

Reacts with

Mouse

Applications

Flow Cyt

applications

Immunogen

Tissue preparation containing Eng protein. The exact immunogen used to generate this antibody is proprietary information.

Q63961

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "0.25-0.5 µg for 10^5-10^8 Cells", "FlowCyt-species-notes": "<p><a href='/en-us/products/unavailable/rat-igg2a-kappa-monoclonal-klhg2a-1-1-pe-isotype-control-ab134674'>ab134674</a> - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.</p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Storage buffer

pH: 7.2 Preservative: 0.09% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD105 also known as endoglin or the CD105 marker is a transmembrane glycoprotein with a molecular weight of approximately 180 kDa. It is a component of the TGF-beta receptor complex and exists in endothelial cells where it is abundantly expressed. Expression of CD105 is higher in proliferating cells particularly in the vasculature. You can also find it in tissues involved in the formation and remodeling of blood vessels such as during angiogenesis.

Biological function summary

Endoglin functions in the regulation of angiogenesis and vascular remodeling. It plays a significant role in mediating cellular responses to TGF-beta signaling influencing endothelial cell proliferation and migration. While not part of a larger structural complex endoglin interacts with receptors and signaling molecules important for vascular development and repair processes. This involvement aids in maintaining endothelial integrity and function under various physiological conditions.

Pathways

CD105 participates in the TGF-beta signaling and angiogenesis pathways. In these pathways it acts in conjunction with other proteins like TGF-beta receptors which play roles in cell differentiation proliferation and apoptosis. The interaction between CD105 and TGF-beta signaling regulates numerous cellular mechanisms impacting angiogenesis and cellular responses to environmental changes.

CD105 has links to hereditary hemorrhagic telangiectasia (HHT) and certain cancers. In HHT mutations in the endoglin gene alter vascular structure leading to the formation of abnormal blood vessels. Oncologically overexpression of CD105 is present in tumor angiogenesis aiding in the progression of certain cancers. Other proteins like VEGF and TGF-beta closely interact with endoglin influencing disease progression and presenting potential targets for therapeutic intervention.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Vascular endothelium glycoprotein that plays an important role in the regulation of angiogenesis (PubMed : 10625534). Required for normal structure and integrity of adult vasculature (By similarity). Regulates the migration of vascular endothelial cells (PubMed : 17540773). Required for normal extraembryonic angiogenesis and for embryonic heart development (PubMed : 10625534). May regulate endothelial cell shape changes in response to blood flow, which drive vascular remodeling and establishment of normal vascular morphology during angiogenesis (PubMed : 28530658). May play a role in the binding of endothelial cells to integrins. Acts as a TGF-beta coreceptor and is involved in the TGF-beta/BMP signaling cascade that ultimately leads to the activation of SMAD transcription factors (PubMed : 23300529). Required for GDF2/BMP9 signaling through SMAD1 in endothelial cells and modulates TGFB1 signaling through SMAD3 (By similarity).

See full target information Eng

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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Journal of cellular biochemistry 122:1791-1804 PubMed34397115

2021

Mesenchymal stem cell and endothelial progenitor cells coinjection improves LPS-induced lung injury via Tie2 activation and downregulation of the TLR4/MyD88 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Sadaf Hoseinnia,Maryam Ghane,Jamile Norouzi,Farzaneh Hosseini

Nature communications 8:16074 PubMed28719575

2017

Non-canonical NOTCH3 signalling limits tumour angiogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Shuheng Lin,Ana Negulescu,Sirisha Bulusu,Benjamin Gibert,Jean-Guy Delcros,Benjamin Ducarouge,Nicolas Rama,Nicolas Gadot,Isabelle Treilleux,Pierre Saintigny,Olivier Meurette,Patrick Mehlen

Stem cells international 2014:576358 PubMed25302065

2014

Osteogenic Potential of Mouse Adipose-Derived Stem Cells Sorted for CD90 and CD105 In Vitro.

Applications

Flow Cyt

Species

Mouse

Maiko Yamamoto,Hidemi Nakata,Jia Hao,Joshua Chou,Shohei Kasugai,Shinji Kuroda

PloS one 8:e75383 PubMed24040409

2013

Amniotic fluid stem cells from EGFP transgenic mice attenuate hyperoxia-induced acute lung injury.

Applications

Unspecified application

Species

Unspecified reactive species

Shih-Tao Wen,Wei Chen,Hsiao-Ling Chen,Cheng-Wei Lai,Chih-Ching Yen,Kun-Hsiung Lee,Shinn-Chih Wu,Chuan-Mu Chen

Cancer research 67:10268-77 PubMed17974968

2007

A role for endoglin as a suppressor of malignancy during mouse skin carcinogenesis.

Applications

Species

Unspecified reactive species

Eduardo Pérez-Gómez,María Villa-Morales,Javier Santos,José Fernández-Piqueras,Carlos Gamallo,Javier Dotor,Carmelo Bernabéu,Miguel Quintanilla

Clinical cancer research : an official journal of the American Association for Cancer Research 13:323-30 PubMed17200371

2007

Monitoring response to anticancer therapy by targeting microbubbles to tumor vasculature.

Applications

IHC-Fr

Species

Unspecified reactive species

Grzegorz Korpanty,Juliet G Carbon,Paul A Grayburn,Jason B Fleming,Rolf A Brekken

Journal of immunology (Baltimore, Md. : 1950) 175:139-46 PubMed15972640

2005

Liver sinusoidal endothelial cells tolerize T cells across MHC barriers in mice.

Applications

IHC-Fr

Species

Unspecified reactive species

Takashi Onoe,Hideki Ohdan,Daisuke Tokita,Masayuki Shishida,Yuka Tanaka,Hidetaka Hara,Wendy Zhou,Kohei Ishiyama,Hiroshi Mitsuta,Kentaro Ide,Toshimasa Asahara

Oncogene 22:5976-85 PubMed12955076

2003

Expression of the TGF-beta coreceptor endoglin in epidermal keratinocytes and its dual role in multistage mouse skin carcinogenesis.

Applications

IHC-Fr, WB

Species

Unspecified reactive species, Unspecified reactive species

Miguel Quintanilla,Jose Ramón Ramirez,Eduardo Pérez-Gómez,Diana Romero,Beatriz Velasco,Michelle Letarte,Jose Miguel López-Novoa,Carmelo Bernabéu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

PE Anti-CD105 antibody [MJ7/18]

Flow Cytometry - PE Anti-CD105 antibody [MJ7/18] (AB93567)

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Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (8)

Product promise