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AB25175

PE Anti-CD11b antibody [M1/70]

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(6 Publications)

Rat Monoclonal CD11b antibody - conjugated to PE. Suitable for Flow Cyt and reacts with Mouse samples. Cited in 6 publications. Immunogen corresponding to Cell preparation containing Itgam protein.

View Alternative Names

CD11b, Integrin alpha-M, CD11 antigen-like family member B, CR-3 alpha chain, Cell surface glycoprotein MAC-1 subunit alpha, Leukocyte adhesion receptor MO1, Itgam

1 Images
Flow Cytometry - PE Anti-CD11b antibody [M1/70] (AB25175)
  • Flow Cyt

Supplier Data

Flow Cytometry - PE Anti-CD11b antibody [M1/70] (AB25175)

C57Bl/6 bone marrow cells stained with 0.125μg ab25175 (solid line) or 0.125μg rat IgG2b PE isotype control (dashed line).

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-CD11b antibody [M1/70]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-CD11b antibody [M1/70]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-CD11b antibody [M1/70]

  • 578 PE

    PE Anti-CD11b antibody [M1/70]

  • Unconjugated

    Anti-CD11b antibody [M1/70]

  • Carrier free

    Anti-CD11b antibody [M1/70] - BSA and Azide free

  • 778 PE/Cy7®

    PE/Cy7® Anti-CD11b antibody [M1/70]

  • 660 APC

    APC Anti-CD11b antibody [M1/70]

  • 450 violetFluor™ 450

    violetFluor™ 450 Anti-CD11b antibody [M1/70]

  • redFluor™ 710

    redFluor™ 710 Anti-CD11b antibody [M1/70]

  • 785 APC/Cy7®

    APC/Cy7® Anti-CD11b antibody [M1/70]

  • Biotin

    Biotin Anti-CD11b antibody [M1/70]

  • 519 FITC

    FITC Anti-CD11b antibody [M1/70]

  • 667 PE/Cy5®

    PE/Cy5® Anti-CD11b antibody [M1/70]

  • 695 PerCP/Cy5.5®

    PerCP/Cy5.5® Anti-CD11b antibody [M1/70]

Key facts

Host species

Rat

Clonality

Monoclonal

Clone number

M1/70

Isotype

IgG2b

Light chain type

kappa

Conjugation

PE

Excitation/Emission

Ex: 480;565nm, Em: 578nm

Carrier free

No

Reacts with

Mouse

Applications

Flow Cyt

applications

Immunogen

Cell preparation containing Itgam protein. The exact immunogen used to generate this antibody is proprietary information.

P05555

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/r-pe-rat-igg2b-kappa-monoclonal-klh-g2b-1-2-isotype-control-ab136585'>ab136585</a> - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody.</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 Preservative: 0.09% Sodium azide Constituents: 0.88% Sodium chloride, 0.12% Sodium dihydrogen phosphate, 0.1% Gelatin
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD11b also known as Integrin alpha M is a protein with a molecular weight of approximately 170 kDa. This protein functions mechanically as an important adhesion molecule. It forms a complex with CD18 to become part of leukocyte-specific beta2 integrins specifically the Mac-1 (CR3) receptor. CD11b is largely expressed on the surface of various myeloid cells including monocytes macrophages granulocytes and a subset of natural killer cells. Researchers often utilize anti-CD11b antibodies and other markers such as APC or CD11b IHC to study its expression in different contexts.
Biological function summary

CD11b plays a critical role in various immune responses by mediating phagocytosis chemotaxis and cell migration. It is essential for proper leukocyte adherence to endothelial cells and for the engulfment of pathogens or apoptotic cells. CD11b as part of the Mac-1 complex contributes to the immune surveillance and inflammatory processes. This complex interacts with diverse ligands like ICAM-1 fibrinogen and complement component iC3b facilitating an immunological response.

Pathways

CD11b is instrumental within the integrin signaling pathway and the Leukocyte Transendothelial Migration pathway. Its interaction with ICAM-1 is central to the leukocyte adhesion cascade influencing the movement of immune cells across endothelial barriers. CD11b also associates with proteins such as Src family kinases and PI3K which activate downstream signaling responsible for the reorganization of the cytoskeleton and cell movement during immune responses.

CD11b is linked to inflammatory conditions like rheumatoid arthritis and systemic lupus erythematosus. Aberrant expression or function of CD11b can result in improper immune regulation contributing to the pathogenesis of these diseases. Its interaction with complement receptor iC3b plays a part in inflammation and tissue damage observed in these conditions. Consequently CD11b represents a potential target for therapeutic intervention in diseases where dysregulated immune activity is a feature.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Integrin ITGAM/ITGB2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles and pathogens (By similarity). It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. Integrin ITGAM/ITGB2 is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain. Regulates neutrophil migration. In association with beta subunit ITGB2/CD18, required for CD177-PRTN3-mediated activation of TNF primed neutrophils (By similarity). May regulate phagocytosis-induced apoptosis in extravasated neutrophils (By similarity). May play a role in mast cell development (By similarity). Required with TYROBP/DAP12 in microglia to control production of microglial superoxide ions which promote the neuronal apoptosis that occurs during brain development (PubMed : 18685038).
See full target information Itgam
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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 14:17241 PubMed39060348

2024

Progesterone modulates the immune microenvironment to suppress ovalbumin-induced airway inflammation by inhibiting NETosis.

Applications

Unspecified application

Species

Unspecified reactive species

Lin Wang,Feng-Ying Huang,Shu-Zhen Dai,Yongshu Fu,Xiangdong Zhou,Cai-Chun Wang,Guang-Hong Tan,Qi Li

PeerJ 12:e17106 PubMed38646478

2024

S100A8/9 modulates perturbation and glycolysis of macrophages in allergic asthma mice.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoyi Ji,Chunhua Nie,Yuan Yao,Yu Ma,Huafei Huang,Chuangli Hao

Journal of cellular biochemistry 122:1791-1804 PubMed34397115

2021

Mesenchymal stem cell and endothelial progenitor cells coinjection improves LPS-induced lung injury via Tie2 activation and downregulation of the TLR4/MyD88 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Sadaf Hoseinnia,Maryam Ghane,Jamile Norouzi,Farzaneh Hosseini

Nature communications 9:4777 PubMed30429468

2018

MR imaging tracking of inflammation-activatable engineered neutrophils for targeted therapy of surgically treated glioma.

Applications

Unspecified application

Species

Unspecified reactive species

Meiying Wu,Haixian Zhang,Changjun Tie,Chunhong Yan,Zhiting Deng,Qian Wan,Xin Liu,Fei Yan,Hairong Zheng

Scientific reports 7:5187 PubMed28701721

2017

TSG-6 Secreted by Human Adipose Tissue-derived Mesenchymal Stem Cells Ameliorates DSS-induced colitis by Inducing M2 Macrophage Polarization in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Woo-Jin Song,Qiang Li,Min-Ok Ryu,Jin-Ok Ahn,Dong Ha Bhang,Yun Chan Jung,Hwa-Young Youn

The American journal of pathology 111:112-24 PubMed6340516

1983

Dendritic cell and macrophage staining by monoclonal antibodies in tissue sections and epidermal sheets.

Applications

Unspecified application

Species

Unspecified reactive species

T J Flotte,T A Springer,G J Thorbecke
View all publications
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