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AB322448

PE Anti-CD11b antibody [M1/70]

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Rat Recombinant Monoclonal CD11b antibody - conjugated to PE. Suitable for Flow Cyt and reacts with Human, Mouse samples.

View Alternative Names

CD11b, CD11B, CR3A, ITGAM, Integrin alpha-M, CD11 antigen-like family member B, CR-3 alpha chain, Cell surface glycoprotein MAC-1 subunit alpha, Leukocyte adhesion receptor MO1, Neutrophil adherence receptor

3 Images
Flow Cytometry - PE Anti-CD11b antibody [M1/70] (AB322448)
  • Flow Cyt

Supplier Data

Flow Cytometry - PE Anti-CD11b antibody [M1/70] (AB322448)

Flow cytometric analysis of Human PBMC (human peripheral blood mononuclear cell) cells labelling CD11b with ab322448 at 1/500 dilution (0.1ug) / Right compared with a Rat IgG isotype control - Phycoerythrin / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Gated on viable cells.

Flow Cytometry - PE Anti-CD11b antibody [M1/70] (AB322448)
  • Flow Cyt

Supplier Data

Flow Cytometry - PE Anti-CD11b antibody [M1/70] (AB322448)

Flow cytometric analysis of Mouse PBMC (mouse peripheral blood mononuclear cell) cells labelling CD11b with ab322448 at 1/500 dilution (0.1ug) / Right compared with a Rat IgG isotype control - Phycoerythrin / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Gated on viable cells.

Flow Cytometry - PE Anti-CD11b antibody [M1/70] (AB322448)
  • Flow Cyt

Supplier Data

Flow Cytometry - PE Anti-CD11b antibody [M1/70] (AB322448)

Flow cytometric analysis of WEHI-231 (Mouse B cell lymphoma B lymphocyte, Left) / RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage, Right) cells labelling CD11b with ab322448 at 1/500 dilution (0.1ug) (Red) compared with a Rat IgG isotype control - Phycoerythrin (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Negative control : WEHI-231 (PMID : 2457584).
Gated on viable cells.

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-CD11b antibody [M1/70]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-CD11b antibody [M1/70]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-CD11b antibody [M1/70]

  • 660 APC

    APC Anti-CD11b antibody [M1/70]

  • 450 violetFluor™ 450

    violetFluor™ 450 Anti-CD11b antibody [M1/70]

  • redFluor™ 710

    redFluor™ 710 Anti-CD11b antibody [M1/70]

  • 785 APC/Cy7®

    APC/Cy7® Anti-CD11b antibody [M1/70]

  • Biotin

    Biotin Anti-CD11b antibody [M1/70]

  • 578 PE

    PE Anti-CD11b antibody [M1/70]

  • 519 FITC

    FITC Anti-CD11b antibody [M1/70]

  • 667 PE/Cy5®

    PE/Cy5® Anti-CD11b antibody [M1/70]

  • 695 PerCP/Cy5.5®

    PerCP/Cy5.5® Anti-CD11b antibody [M1/70]

  • 778 PE/Cy7®

    PE/Cy7® Anti-CD11b antibody [M1/70]

  • Unconjugated

    Anti-CD11b antibody [M1/70]

  • Carrier free

    Anti-CD11b antibody [M1/70] - BSA and Azide free

Key facts

Host species

Rat

Clonality

Monoclonal

Clone number

M1/70

Isotype

IgG

Conjugation

PE

Excitation/Emission

Ex: 480;565nm, Em: 578nm

Carrier free

No

Reacts with

Human, Mouse

Applications

Flow Cyt

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/500", "FlowCyt-species-notes": "<p></p>" }, "Mouse": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/500", "FlowCyt-species-notes": "<p></p>" } } }
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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD11b also known as Integrin alpha M is a protein with a molecular weight of approximately 170 kDa. This protein functions mechanically as an important adhesion molecule. It forms a complex with CD18 to become part of leukocyte-specific beta2 integrins specifically the Mac-1 (CR3) receptor. CD11b is largely expressed on the surface of various myeloid cells including monocytes macrophages granulocytes and a subset of natural killer cells. Researchers often utilize anti-CD11b antibodies and other markers such as APC or CD11b IHC to study its expression in different contexts.
Biological function summary

CD11b plays a critical role in various immune responses by mediating phagocytosis chemotaxis and cell migration. It is essential for proper leukocyte adherence to endothelial cells and for the engulfment of pathogens or apoptotic cells. CD11b as part of the Mac-1 complex contributes to the immune surveillance and inflammatory processes. This complex interacts with diverse ligands like ICAM-1 fibrinogen and complement component iC3b facilitating an immunological response.

Pathways

CD11b is instrumental within the integrin signaling pathway and the Leukocyte Transendothelial Migration pathway. Its interaction with ICAM-1 is central to the leukocyte adhesion cascade influencing the movement of immune cells across endothelial barriers. CD11b also associates with proteins such as Src family kinases and PI3K which activate downstream signaling responsible for the reorganization of the cytoskeleton and cell movement during immune responses.

CD11b is linked to inflammatory conditions like rheumatoid arthritis and systemic lupus erythematosus. Aberrant expression or function of CD11b can result in improper immune regulation contributing to the pathogenesis of these diseases. Its interaction with complement receptor iC3b plays a part in inflammation and tissue damage observed in these conditions. Consequently CD11b represents a potential target for therapeutic intervention in diseases where dysregulated immune activity is a feature.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Integrin ITGAM/ITGB2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles and pathogens (PubMed : 20008295, PubMed : 9558116). It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. Integrin ITGAM/ITGB2 is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain. Regulates neutrophil migration (PubMed : 28807980). In association with beta subunit ITGB2/CD18, required for CD177-PRTN3-mediated activation of TNF primed neutrophils (PubMed : 21193407). May regulate phagocytosis-induced apoptosis in extravasated neutrophils (By similarity). May play a role in mast cell development (By similarity). Required with TYROBP/DAP12 in microglia to control production of microglial superoxide ions which promote the neuronal apoptosis that occurs during brain development (By similarity).
See full target information ITGAM
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