AB28101

PE Anti-CD11b antibody [MEM-174]

Name: PE Anti-CD11b antibody [MEM-174] (ab28101) | Abcam
Brand: Abcam Limited
SKU: AB28101
Price: 170 USD
Availability: InStock
Rating: 5 (3 reviews)

(3 Reviews)

(2 Publications)

Mouse Monoclonal CD11b antibody - conjugated to PE. Suitable for Flow Cyt and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Cell preparation containing ITGAM protein.

View Alternative Names

CD11b, CD11B, CR3A, ITGAM, Integrin alpha-M, CD11 antigen-like family member B, CR-3 alpha chain, Cell surface glycoprotein MAC-1 subunit alpha, Leukocyte adhesion receptor MO1, Neutrophil adherence receptor

2 Images

Flow Cytometry - PE Anti-CD11b antibody [MEM-174] (AB28101)

Flow Cyt

Supplier Data

Flow Cytometry - PE Anti-CD11b antibody [MEM-174] (AB28101)

Flow cytometry analysis of human peripheral blood cells labelling CD11b with ab28101.

Flow Cyt

AbReview38434****

Flow Cytometry - PE Anti-CD11b antibody [MEM-174] (AB28101)

ab28101 staining CD11b in Human peripheral blood cells by Flow Cytometry. The sample was incubated with the primary antibody (1/6) for 30 minutes at 4°C. Gating Strategy : Granulocytes.

This image is courtesy of an Abreview submitted by Ina Laura Pieper

660 APC

APC Anti-CD11b antibody [MEM-174]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

MEM-174

Isotype

IgG2a

Conjugation

Excitation/Emission

Ex: 480;565nm, Em: 578nm

Carrier free

Reacts with

Human

Applications

Flow Cyt

applications

Immunogen

Cell preparation containing ITGAM protein. The exact immunogen used to generate this antibody is proprietary information.

P11215

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "20 µL for 10^6 Cells", "FlowCyt-species-notes": "Use 20μl for 106 cells or 100μl of whole blood.<a href='/en-us/products/primary-antibodies/pe-mouse-igg2a-b12-8-isotype-control-ab91363'>ab91363</a> - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody." } } }

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Product details

The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use.

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Properties and storage information

Form

Liquid

Purity

IgG fraction

Purification notes

Size exclusion chromatography.

Storage buffer

pH: 7.4 Preservative: 0.097% Sodium azide Constituents: PBS, 0.2% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD11b also known as Integrin alpha M is a protein with a molecular weight of approximately 170 kDa. This protein functions mechanically as an important adhesion molecule. It forms a complex with CD18 to become part of leukocyte-specific beta2 integrins specifically the Mac-1 (CR3) receptor. CD11b is largely expressed on the surface of various myeloid cells including monocytes macrophages granulocytes and a subset of natural killer cells. Researchers often utilize anti-CD11b antibodies and other markers such as APC or CD11b IHC to study its expression in different contexts.

Biological function summary

CD11b plays a critical role in various immune responses by mediating phagocytosis chemotaxis and cell migration. It is essential for proper leukocyte adherence to endothelial cells and for the engulfment of pathogens or apoptotic cells. CD11b as part of the Mac-1 complex contributes to the immune surveillance and inflammatory processes. This complex interacts with diverse ligands like ICAM-1 fibrinogen and complement component iC3b facilitating an immunological response.

Pathways

CD11b is instrumental within the integrin signaling pathway and the Leukocyte Transendothelial Migration pathway. Its interaction with ICAM-1 is central to the leukocyte adhesion cascade influencing the movement of immune cells across endothelial barriers. CD11b also associates with proteins such as Src family kinases and PI3K which activate downstream signaling responsible for the reorganization of the cytoskeleton and cell movement during immune responses.

CD11b is linked to inflammatory conditions like rheumatoid arthritis and systemic lupus erythematosus. Aberrant expression or function of CD11b can result in improper immune regulation contributing to the pathogenesis of these diseases. Its interaction with complement receptor iC3b plays a part in inflammation and tissue damage observed in these conditions. Consequently CD11b represents a potential target for therapeutic intervention in diseases where dysregulated immune activity is a feature.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Integrin ITGAM/ITGB2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles and pathogens (PubMed : 20008295, PubMed : 9558116). It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. Integrin ITGAM/ITGB2 is also a receptor for fibrinogen and factor X. It recognizes P1 and P2 peptides of fibrinogen gamma chain. Regulates neutrophil migration (PubMed : 28807980). In association with beta subunit ITGB2/CD18, required for CD177-PRTN3-mediated activation of TNF primed neutrophils (PubMed : 21193407). Integrin ITGAM/ITGB2 is also a receptor for ICAM1 ligand ensuring adhesion between stimulated neutrophils and stimulated endothelial cells (PubMed : 1980124). May regulate phagocytosis-induced apoptosis in extravasated neutrophils (By similarity). May play a role in mast cell development (By similarity). Required with TYROBP/DAP12 in microglia to control production of microglial superoxide ions which promote the neuronal apoptosis that occurs during brain development (By similarity).

See full target information ITGAM

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Acta pharmaceutica Sinica. B 9:794-808 PubMed31384539

2019

Development of the triazole-fused pyrimidine derivatives as highly potent and reversible inhibitors of histone lysine specific demethylase 1 (LSD1/KDM1A).

Applications

Unspecified application

Species

Unspecified reactive species

Zhonghua Li,Lina Ding,Zhongrui Li,Zhizheng Wang,Fengzhi Suo,Dandan Shen,Taoqian Zhao,Xudong Sun,Junwei Wang,Ying Liu,Liying Ma,Bing Zhao,Pengfei Geng,Bin Yu,Yichao Zheng,Hongmin Liu

International journal of molecular medicine 42:2193-2202 PubMed30015833

2018

Regenerative potential of human nucleus pulposus resident stem/progenitor cells declines with ageing and intervertebral disc degeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Hao Wu,Yupan Shang,Jiayue Yu,Xiaoli Zeng,Jinhua Lin,Mei Tu,Lek Hang Cheang,Jiaqing Zhang

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

PE Anti-CD11b antibody [MEM-174]

Flow Cytometry - PE Anti-CD11b antibody [MEM-174] (AB28101)

Flow Cytometry - PE Anti-CD11b antibody [MEM-174] (AB28101)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purity

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise