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AB316176

PE Anti-CD11c antibody [KB90]

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Mouse Recombinant Monoclonal CD11c antibody - conjugated to PE. Suitable for Flow Cyt and reacts with Human, Mouse samples.

View Alternative Names

CD11c, CD11C, ITGAX, Integrin alpha-X, CD11 antigen-like family member C, Leu M5

2 Images
Flow Cytometry - PE Anti-CD11c antibody [KB90] (AB316176)
  • Flow Cyt

Lab

Flow Cytometry - PE Anti-CD11c antibody [KB90] (AB316176)

Flow cytometry staining of human peripheral blood mononuclear cells (PBMCs) with ab316176 (right) or Rabbit IgG (monoclonal) Phycoerythrin Isotype Control (ab209478) (left). PBMCs were incubated for 30 min on ice in 1x PBS containing 10 μg/ml human IgG and 10 % normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody ab316176 or Rabbit IgG (monoclonal) Phycoerythrin Isotype Control (ab209478) (1x 106 in 100 μl at 0.008 μg/ml (1/62500)) for 30min on ice. The cells were simultaneously stained with CD3.

Acquisition of >30000 events were collected using a 50 mW Yellow/Green laser (561nm) and 585/42 bandpass filter. Events were gated on viable cells.

Flow Cytometry - PE Anti-CD11c antibody [KB90] (AB316176)
  • Flow Cyt

Lab

Flow Cytometry - PE Anti-CD11c antibody [KB90] (AB316176)

Flow cytometry staining of human peripheral blood mononuclear cells (PBMCs) with ab316176 (right) or Rabbit IgG (monoclonal) Phycoerythrin Isotype Control (ab209478) (left). PBMCs were incubated for 30 min on ice in 1x PBS containing 10 μg/ml human IgG and 10 % normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody ab316176 or Rabbit IgG (monoclonal) Phycoerythrin Isotype Control (ab209478) (1x 106 in 100 μl at 0.008 μg/ml (1/62500)) for 30min on ice. The cells were simultaneously stained with CD11b.

Acquisition of >30000 events were collected using a 50 mW Yellow/Green laser (561nm) and 585/42 bandpass filter. Events were gated on viable cells.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

KB90

Isotype

IgG1

Conjugation

PE

Excitation/Emission

Ex: 480;565nm, Em: 578nm

Carrier free

No

Reacts with

Human, Human, Mouse

Applications

Flow Cyt

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/62500", "FlowCyt-species-notes": "<p></p>" }, "Mouse": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>" } } }
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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD11c also known as ITGAX is a subunit of integrin proteins that plays a pivotal role in immune cell function. This protein has a molecular mass of about 150 kDa and is primarily expressed on the surface of dendritic cells monocytes and macrophages. It serves as a marker for these cells commonly utilized in applications like CD11c flow cytometry and CD11c IHC. Researchers often study this target using CD11c antibodies to explore immune system dynamics and cell surface characteristics.
Biological function summary

CD11c functions as an integrin alpha X subunit forming a heterodimer with the beta 2 subunit known as ITGB2. This complex is important for cell adhesion and migration processes. It mediates the binding of leukocytes to endothelial cells facilitating their migration from the bloodstream to sites of inflammation or infection. CD11c's presence on immune cells makes it essential for presenting antigens and initiating immune responses therefore having critical implications for immunity.

Pathways

CD11c plays a significant role in the immune system particularly in the leukocyte adhesion and migration pathways. It partners with proteins like ITGB2 to mediate these functions. The signaling pathways involving CD11c and related molecules regulate leukocyte trafficking and activation ensuring proper localization and function at sites of damage or infection. These pathways are essential for maintaining immune homeostasis and prompt responses to pathogens.

CD11c has significant connections to autoimmune diseases and chronic inflammatory conditions. Aberrant CD11c expression or activity can contribute to the pathogenesis of conditions like rheumatoid arthritis where leukocyte migration and persistent inflammation play a role. Studies also link CD11c with proteins involved in these disorders highlighting its importance in disease progression and potential as a therapeutic target. The understanding of CD11c's role in these conditions can guide the development of treatments aimed at modulating immune responses.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Integrin alpha-X/beta-2 is a receptor for fibrinogen. It recognizes the sequence G-P-R in fibrinogen. It mediates cell-cell interaction during inflammatory responses. It is especially important in monocyte adhesion and chemotaxis. Functions as a receptor of the erythrocyte-specific adhesion molecule ICAM4 and mediates erythrophagocytosis (PubMed : 16985175).
See full target information ITGAX
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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