AB210309

PE Anti-CD11c antibody [N418]

Name: PE Anti-CD11c antibody [N418] (ab210309) | Abcam
Brand: Abcam Limited
SKU: AB210309

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(2 Publications )

Anti-CD11c antibody [N418] conjugated to PE - suitable for flow cytometry, and reacts with mouse samples. Clone [N418] is a Armenian Hamster monoclonal antibody and one of the most commonly used clones for flow cytometry.

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1 Images

Flow Cytometry - PE Anti-CD11c antibody [N418] (AB210309)

Flow Cyt

Supplier Data

Flow Cytometry - PE Anti-CD11c antibody [N418] (AB210309)

Flow cytometric analysis of C57Bl/6 splenocytes labeling CD11c with APC Anti-Mouse MHC Class II and 0.25 ug ab210309 (right panel) or 0.25 ug PE Armenian Hamster IgG (left panel).

519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-CD11c antibody [N418]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-CD11c antibody [N418]

Key facts

Host species

Armenian hamster

Clonality

Monoclonal

Clone number

N418

Isotype

IgG

Conjugation

Excitation/Emission

Ex: 480;565nm, Em: 578nm

Carrier free

Reacts with

Mouse

Applications

Flow Cyt

applications

Immunogen

Cell preparation containing Itgax protein. The exact immunogen used to generate this antibody is proprietary information.

Q9QXH4

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p>Suggested use 0.25 μg.</p>" } } }

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Properties and storage information

Form

Liquid

Purification notes

ab210309 was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.

Storage buffer

pH: 7.2 Preservative: 0.09% Sodium azide Constituents: 0.87% Sodium chloride, 0.12% Sodium dihydrogen phosphate, 0.1% Gelatin

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Store in the dark

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD11c also known as ITGAX is a subunit of integrin proteins that plays a pivotal role in immune cell function. This protein has a molecular mass of about 150 kDa and is primarily expressed on the surface of dendritic cells monocytes and macrophages. It serves as a marker for these cells commonly utilized in applications like CD11c flow cytometry and CD11c IHC. Researchers often study this target using CD11c antibodies to explore immune system dynamics and cell surface characteristics.

Biological function summary

CD11c functions as an integrin alpha X subunit forming a heterodimer with the beta 2 subunit known as ITGB2. This complex is important for cell adhesion and migration processes. It mediates the binding of leukocytes to endothelial cells facilitating their migration from the bloodstream to sites of inflammation or infection. CD11c's presence on immune cells makes it essential for presenting antigens and initiating immune responses therefore having critical implications for immunity.

Pathways

CD11c plays a significant role in the immune system particularly in the leukocyte adhesion and migration pathways. It partners with proteins like ITGB2 to mediate these functions. The signaling pathways involving CD11c and related molecules regulate leukocyte trafficking and activation ensuring proper localization and function at sites of damage or infection. These pathways are essential for maintaining immune homeostasis and prompt responses to pathogens.

CD11c has significant connections to autoimmune diseases and chronic inflammatory conditions. Aberrant CD11c expression or activity can contribute to the pathogenesis of conditions like rheumatoid arthritis where leukocyte migration and persistent inflammation play a role. Studies also link CD11c with proteins involved in these disorders highlighting its importance in disease progression and potential as a therapeutic target. The understanding of CD11c's role in these conditions can guide the development of treatments aimed at modulating immune responses.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Integrin alpha-X/beta-2 is a receptor for fibrinogen. It recognizes the sequence G-P-R in fibrinogen. It mediates cell-cell interaction during inflammatory responses. It is especially important in monocyte adhesion and chemotaxis. Functions as a receptor of the erythrocyte-specific adhesion molecule ICAM4 and mediates erythrophagocytosis.

See full target information Itgax

Alternative Names

CD11c, Integrin alpha-X, CD11 antigen-like family member C, Itgax

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Obesity facts 17:24-36 PubMed37820603

2023

Ube2L6 Promotes M1 Macrophage Polarization in High-Fat Diet-Fed Obese Mice via ISGylation of STAT1 to Trigger STAT1 Activation.

Applications

Unspecified application

Species

Unspecified reactive species

Yunqian Li,Xiao Dong,Wenqian He,Huibiao Quan,Kaining Chen,Chaoping Cen,Weiping Wei

Oncology letters 12:1101-1106 PubMed27446401

2016

generation of cytotoxic T lymphocyte response using dendritic cell immunotherapy in osteosarcoma.

Applications

Unspecified application

Species

Unspecified reactive species

Ye-Teng He,Qing-Min Zhang,Quan-Chun Kou,Bo Tang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

PE Anti-CD11c antibody [N418]

Flow Cytometry - PE Anti-CD11c antibody [N418] (AB210309)

Related conjugates and formulations (2)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Alternative Names

Publications (2)

Product promise