AB69775

PE Anti-CD13 antibody [WM15]

Name: Anti-CD13 PE antibody [WM15] (ab69775) Mouse monoclonal IgG1 | Abcam
Brand: Abcam Limited
SKU: AB69775
Price: 110 USD
Availability: InStock

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(6 Publications)

Mouse Monoclonal CD13 antibody - conjugated to PE. Suitable for Flow Cyt and reacts with Human samples. Cited in 6 publications. Immunogen corresponding to Cell preparation containing ANPEP protein.

View Alternative Names

CD13, APN, PEPN, ANPEP, Aminopeptidase N, AP-N, hAPN, Alanyl aminopeptidase, Aminopeptidase M, Microsomal aminopeptidase, Myeloid plasma membrane glycoprotein CD13, gp150, AP-M

1 Images

Flow Cytometry - PE Anti-CD13 antibody [WM15] (AB69775)

Flow Cyt

Supplier Data

Flow Cytometry - PE Anti-CD13 antibody [WM15] (AB69775)

Flow cytometry analysis (surface staining) of human peripheral blood leukocytes using ab69775.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

WM15

Isotype

IgG1

Conjugation

Excitation/Emission

Ex: 480;565nm, Em: 578nm

Carrier free

Reacts with

Human

Applications

Flow Cyt

applications

Immunogen

Cell preparation containing ANPEP protein. The exact immunogen used to generate this antibody is proprietary information.

P15144

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p>or 100 ul of whole blood. <a href='/en-us/products/primary-antibodies/pe-mouse-igg1-b11-6-isotype-control-ab91357'>ab91357</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>" }, "Primates": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" } } }

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Product details

The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions.

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Properties and storage information

Form

Liquid

Purification technique

Size-exclusion chromatography

Storage buffer

pH: 7.4 Preservative: 0.097% Sodium azide Constituents: PBS, 0.2% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD13 also known as aminopeptidase N (ANPEP) is a transmembrane protein with a molecular weight of approximately 150 kDa. It functions as a zinc-dependent metalloenzyme which cleaves N-terminal amino acids from peptides and proteins. CD13 protein is expressed on various cell types including myeloid cells epithelial cells endothelial cells and fibroblasts. Its presence is significantly observed in the brush border of the small intestinal mucosa and the renal proximal tubule. Researchers can study CD13 using anti-CD13 antibodies and CD13 ELISA kits.

Biological function summary

CD13 regulates peptide-mediated signaling and controls the maturation and catabolism of bioactive peptides. The enzymatic function of CD13 influences processes such as cell proliferation motility and angiogenesis. It does not operate as part of a larger complex but its activity modulates several cellular and systemic functions. CD13's role in these biological processes highlights its importance in modulating local and systemic peptide pools which contributes to its diverse physiological effects.

Pathways

CD13 plays significant roles in the renin-angiotensin system and the regulation of inflammatory responses. In the renin-angiotensin system CD13 modulates the activity of angiotensin influencing blood pressure and fluid balance. Through its enzymatic activity CD13 interacts with proteins such as ACE another critical player in this pathway. In inflammation CD13 regulates the availability of chemotactic peptides affecting leukocyte migration and adhesion. The proteins it works with in inflammatory pathways include cytokines which CD13 indirectly modulates by altering chemokine activity.

Aberrant CD13 expression and activity are implicated in cancer and inflammatory diseases. In cancer overexpression of CD13 correlates with tumor growth and metastasis particularly in cancers like renal cell carcinoma and prostate cancer. It can confer an increased malignant phenotype by promoting angiogenesis and immune evasion. In inflammatory disorders dysregulation of CD13 exacerbates diseases such as rheumatoid arthritis through its impact on peptide-mediated signaling and immune cell movement. Its connection with inflammatory cytokines like IL-8 indicates its important role in mediating inflammatory responses.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Broad specificity aminopeptidase which plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases. Also involved in the processing of various peptides including peptide hormones, such as angiotensin III and IV, neuropeptides, and chemokines. May also be involved the cleavage of peptides bound to major histocompatibility complex class II molecules of antigen presenting cells. May have a role in angiogenesis and promote cholesterol crystallization. May have a role in amino acid transport by acting as binding partner of amino acid transporter SLC6A19 and regulating its activity (By similarity).. (Microbial infection) Acts as a receptor for human coronavirus 229E/HCoV-229E. In case of human coronavirus 229E (HCoV-229E) infection, serves as receptor for HCoV-229E spike glycoprotein.. (Microbial infection) Mediates as well Human cytomegalovirus (HCMV) infection.

See full target information ANPEP

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Journal of the American Society of Nephrology : JASN 33:949-965 PubMed35197326

2022

Serum Protein Exposure Activates a Core Regulatory Program Driving Human Proximal Tubule Injury.

Applications

Unspecified application

Species

Unspecified reactive species

Kevin A Lidberg,Selvaraj Muthusamy,Mohamed Adil,Anish Mahadeo,Jade Yang,Ranita S Patel,Lu Wang,Theo K Bammler,Jonathan Reichel,Catherine K Yeung,Jonathan Himmelfarb,Edward J Kelly,Shreeram Akilesh

Translational oncology 11:1271-1282 PubMed30125801

2018

Aminopeptidase N (CD13): Expression, Prognostic Impact, and Use as Therapeutic Target for Tissue Factor Induced Tumor Vascular Infarction in Soft Tissue Sarcoma.

Applications

Unspecified application

Species

Unspecified reactive species

Torsten Kessler,Ariane Baumeier,Caroline Brand,Michael Grau,Linus Angenendt,Saliha Harrach,Ursula Stalmann,Lars Henning Schmidt,Georg Gosheger,Jendrik Hardes,Dimosthenis Andreou,Johannes Dreischalück,Georg Lenz,Eva Wardelmann,Rolf M Mesters,Christian Schwöppe,Wolfgang E Berdel,Wolfgang Hartmann,Christoph Schliemann

PloS one 12:e0177146 PubMed28604784

2017

Potential therapeutic impact of CD13 expression in non-small cell lung cancer.

Applications

Flow Cyt

Species

Human

Lars Henning Schmidt,Caroline Brand,Janine Stucke-Ring,Christoph Schliemann,Torsten Kessler,Saliha Harrach,Michael Mohr,Dennis Görlich,Alessandro Marra,Ludger Hillejan,Carsten Müller-Tidow,Georg Lenz,Eva Wardelmann,Rainer Wiewrodt,Wolfgang E Berdel,Christian Schwöppe,Wolfgang Hartmann

Experimental hematology 21:1695-701 PubMed7902291

1993

CD13 (GP150; aminopeptidase-N): predominant functional activity in blood is localized to plasma and is not cell-surface associated.

Applications

Unspecified application

Species

Unspecified reactive species

E J Favaloro,T Browning,D Facey

British journal of haematology 61:11-20 PubMed4052321

1985

Myeloid progenitor surface antigen identified by monoclonal antibody.

Applications

Unspecified application

Species

Unspecified reactive species

K F Bradstock,E J Favaloro,A Kabral,A Kerr,W G Hughes,E Musgrove

Pathology 17:392-9 PubMed3865147

1985

Human myeloid differentiation antigens identified by monoclonal antibodies: expression on leukemic cells.

Applications

Unspecified application

Species

Unspecified reactive species

K F Bradstock,E J Favaloro,A Kabral,A Kerr,W G Hughes,M C Berndt,E Musgrove

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

PE Anti-CD13 antibody [WM15]

Flow Cytometry - PE Anti-CD13 antibody [WM15] (AB69775)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (6)

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Product promise