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AB253271

PE Anti-CD133 antibody [293C3]

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(5 Publications)

Mouse Monoclonal CD133 antibody - conjugated to PE. Suitable for Flow Cyt and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Full Length Protein corresponding to Human PROM1.

View Alternative Names

CD133, PROML1, MSTP061, PROM1, Prominin-1, Antigen AC133, Prominin-like protein 1

1 Images
Flow Cytometry - PE Anti-CD133 antibody [293C3] (AB253271)
  • Flow Cyt

Supplier Data

Flow Cytometry - PE Anti-CD133 antibody [293C3] (AB253271)

Flow Cytometry analysis of human peripheral blood labeling CD133 with ab253271. Surface staining.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

293C3

Isotype

IgG2b

Conjugation

PE

Excitation/Emission

Ex: 480;565nm, Em: 578nm

Carrier free

No

Reacts with

Human

Applications

Flow Cyt

applications

Immunogen

Full Length Protein corresponding to Human PROM1. The exact immunogen used to generate this antibody is proprietary information.

O43490

Epitope

extracellular

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "10 µL for 10^6 Cells", "FlowCyt-species-notes": "<p>Or 100 μl of whole blood.</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Size-exclusion chromatography
Purification notes
The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use.
Storage buffer
pH: 7.4 Preservative: 0.0975% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD133 also known as Prominin-1 is a transmembrane glycoprotein with a molecular weight of approximately 120 kDa. This protein stands out due to its expression in various stem and progenitor cells including those of the neural hematopoietic and epithelial lineages. CD133 is recognized for its distinct five transmembrane domains and two large extracellular loops. Researchers commonly use CD133 as a cell surface marker to identify stem and progenitor cells. The significance of CD133 extends to immunohistochemistry (IHC) and Western blot applications where it is a popular target for detection and analysis.
Biological function summary

CD133 is involved in maintaining the cellular architecture and signaling processes. It plays a role in cellular differentiation and proliferation while acting as a part of larger protein complexes on the cell surface. This protein is notable as it contributes significantly to the regulation of stem cell properties supporting the growth and repair in various tissues. Anti-CD133 antibodies are valuable tools in studying these functions due to their specificity in targeting this marker.

Pathways

CD133 participates in cellular signaling and transport pathways vital for intercellular communication and homeostasis. It interacts within pathways related to cell polarity and asymmetric division both essential for tissue development and maintenance. CD133 is associated with proteins like CD133/1 and CD133/2 which further highlights its involvement in these complex biological networks and on pathways such as the Wnt signaling pathway which regulates gene expression associated with cell proliferation and differentiation.

Several studies indicate a strong relationship between CD133 expression and certain cancers such as glioblastoma and colorectal cancer. In these cases CD133 serves not only as a marker but also as an indicator of the presence of cancer stem cells which are capable of tumor initiation and maintenance. Furthermore researchers have identified connections between CD133 and other proteins such as c-Met (cMab) within these pathological contexts suggesting interactions that could contribute to disease progression and offering potential targets for therapeutic intervention.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

May play a role in cell differentiation, proliferation and apoptosis (PubMed : 24556617). Binds cholesterol in cholesterol-containing plasma membrane microdomains and may play a role in the organization of the apical plasma membrane in epithelial cells. During early retinal development acts as a key regulator of disk morphogenesis. Involved in regulation of MAPK and Akt signaling pathways. In neuroblastoma cells suppresses cell differentiation such as neurite outgrowth in a RET-dependent manner (PubMed : 20818439).
See full target information PROM1
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in immunology 16:1580963 PubMed40969742

2025

Penicillamine ameliorates intestinal barrier damage in dextran sulfate sodium-induced experimental colitis mice by inhibiting cuproptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Jiaze Ma,Yimeng Ma,Shuangshuang Wang,Wenwen Tang,Chen Chen,Yang Li,Yugen Chen,Tuo Chen

Journal of neuro-oncology 175:255-265 PubMed40553392

2025

Glioblastoma cell motility and invasion is regulated by membrane-associated heat shock protein Hsp70.

Applications

Unspecified application

Species

Unspecified reactive species

Ruslana Likhomanova,Elena Oganesyan,Natalia Yudintceva,Georgii Fofanov,Anastasiia Nechaeva,Alexei Ulitin,Aleksander Kim,Nikolay Aksenov,Alla Shatrova,Rustam Ziganshin,Danila Bobkov,Konstantin Samochernykh,Stephanie E Combs,Maxim Shevtsov

Cancer biology & medicine 20: PubMed37731205

2023

The inducible secreting TLR5 agonist, CBLB502, enhances the anti-tumor activity of CAR133-NK92 cells in colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaohui Wang,Wei Qiu,Haoyu Liu,Min He,Wei He,Zhan Li,Zhiqiang Wu,Xiang Xu,Ping Chen

Biology open 11: PubMed35023542

2022

A human stem cell resource to decipher the biochemical and cellular basis of neurodevelopmental defects in Lowe syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Bilal M Akhtar,Priyanka Bhatia,Shubhra Acharya,Sanjeev Sharma,Yojet Sharma,Aswathy Bhuvanendran Nair Suseela Devi,Kavina Ganapathy,Anil Vasudevan,Padinjat Raghu

Frontiers in oncology 11:711735 PubMed34646768

2021

OTUB2 Facilitates Tumorigenesis of Gastric Cancer Through Promoting KDM1A-Mediated Stem Cell-Like Properties.

Applications

Unspecified application

Species

Unspecified reactive species

Guangming Liu,Wei Guo,Junjie Qin,Zhiliang Lin
View all publications
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