Mouse Monoclonal CD137 antibody - conjugated to PE. Suitable for Flow Cyt and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human TNFRSF9.
Images
![Flow Cytometry - PE Anti-CD137 antibody [4B4-1] (AB200561), expandable thumbnail](https://content.abcam.com/products/images/pe-cd137-antibody-4b4-1-ab200561--flow-cytometry-img74974.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - PE Anti-CD137 antibody [4B4-1] (AB200561)")
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Conjugation
- PE
- Excitation/Emission
- Ex: 480;565nm, Em: 578nm
- Storage buffer
pH: 7.4
Preservative: 0.098% Sodium azide
Constituents: 99% PBS, 0.2% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Recombinant Fragment Protein within Human TNFRSF9. The exact immunogen used to generate this antibody is proprietary information. Database link Q07011
Reactivity data
Select an application| Flow Cyt | |
|---|---|
| Human | Tested |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 10 µL for 106 Cells | Notes 10 μl / 100 μl of whole blood |
Associated Products
Select an associated product type
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Target data
Function
Receptor for TNFSF9/4-1BBL. Conveys a signal that enhances CD8(+) T-cell survival, cytotoxicity, and mitochondrial activity, thereby promoting immunity against viruses and tumors (Probable).
Alternative names
CD137, ILA, TNFRSF9, Tumor necrosis factor receptor superfamily member 9, 4-1BB ligand receptor, CDw137, T-cell antigen 4-1BB homolog, T-cell antigen ILA
Recommended products
Mouse Monoclonal CD137 antibody - conjugated to PE. Suitable for Flow Cyt and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human TNFRSF9.
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Conjugation
- PE
- Excitation/Emission
- Ex: 480;565nm, Em: 578nm
- Storage buffer
pH: 7.4
Preservative: 0.098% Sodium azide
Constituents: 99% PBS, 0.2% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Recombinant Fragment Protein within Human TNFRSF9. The exact immunogen used to generate this antibody is proprietary information. Database link Q07011
- Clone number
- 4B4-1
- Purification technique
- Size-exclusion chromatography
- Concentration
- Purification notes
ab200561 is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze, Store in the dark
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CD137 also known as 4-1BB or TNFRSF9 is a protein that functions as a costimulatory receptor in the immune system. It belongs to the tumor necrosis factor receptor (TNFR) superfamily. CD137 has a mass of approximately 30 kDa. Expression of CD137 occurs mainly on activated T lymphocytes including both CD8+ and CD4+ T cells as well as natural killer (NK) cells dendritic cells and monocytes. The CD137 ligand (CD137L) is the counterpart that engages this receptor transmitting signals important for immune response regulation.
- Biological function summary
CD137 plays an important role in T cell activation and survival. It is a member of a receptor-ligand system that enhances immune cell functions. CD137 is part of a receptor complex that when bound by its ligand CD137L promotes cell proliferation and cytokine production. It also supports the development of memory T cells contributing to the body's ability to mount long-term immune responses.
- Pathways
CD137 takes part in the NF-kB signaling and MAPK pathways. These pathways are vital for regulating immune responses and inflammation. CD137 signaling through these pathways involves interaction with proteins such as TRAF1 and TRAF2 which help mediate downstream effects critical for cell survival and proliferation. These interactions enhance the body's defense against pathogens and tumors.
- Associated diseases and disorders
CD137 is associated with cancer immunotherapy and autoimmune diseases. In cancer CD137 enhances the anti-tumor activity of immune cells making it a target for immunotherapy strategies. For autoimmune diseases abnormal CD137 expression can lead to excessive immune responses contributing to disease pathogenesis. The protein is also connected to TNF-alpha through its involvement in inflammation suggesting potential intervention points for modulating immune response in related diseases.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
![Flow Cytometry - PE Anti-CD137 antibody [4B4-1] (ab200561), expandable thumbnail](https://content.abcam.com/products/images/pe-cd137-antibody-4b4-1-ab200561--flow-cytometry-img74974.jpg "Flow Cytometry - PE Anti-CD137 antibody [4B4-1] (ab200561)")
Flow Cytometry - PE Anti-CD137 antibody [4B4-1] (ab200561)
CD137 Flow Cytometry staining using mouse Anti-CD137 antibody
Flow cytometry analysis (surface staining) of CD137 in PHA activated PBMC (peripheral blood mononuclear cell) with ab200561 (red), and mouse IgG1 control isotope (blue).
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