Mouse Monoclonal CD163 antibody - conjugated to PE. Suitable for Flow Cyt and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Cell preparation containing CD163 protein.
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![Flow Cytometry - PE Anti-CD163 antibody [GHI/61] (AB95613), expandable thumbnail](https://content.abcam.com/products/images/pe-cd163-antibody-ghi-61-ab95613--flow-cytometry-img58643.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - PE Anti-CD163 antibody [GHI/61] (AB95613)")
Publications
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- Nature communications 14:76392023Acoustofluidic Interfaces for the Mechanobiological Secretome of MSCs.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYe He,Shujie Yang,Pengzhan Liu,Ke Li,Ke Jin,Ryan Becker,Jinxin Zhang,Chuanchuan Lin,Jianping Xia,Zhehan Ma,Zhiteng Ma,Ruoyu Zhong,Luke P Lee,Tony Jun HuangPubMed 37993431
- Aging 12:6928-69462020AKT3 deficiency in M2 macrophages impairs cutaneous wound healing by disrupting tissue remodeling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSong Gu et. al.PubMed 32291381
- American journal of physiology. Heart and circulat 299:H656-632010Adiponectin primes human monocytes into alternative anti-inflammatory M2 macrophages.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFina Lovren et. al.PubMed 20622108
- European journal of immunology 23:2320-51993A new macrophage differentiation antigen which is a member of the scavenger receptor superfamily.Applications:Unspecified applicationReactive species:Unspecified reactive speciesS K Law,K J Micklem,J M Shaw,X P Zhang,Y Dong,A C Willis,D Y MasonPubMed 8370408
- Immunology 75:588-951992A monocyte/macrophage antigen recognized by the four antibodies GHI/61, Ber-MAC3, Ki-M8 and SM4.Applications:Flow CytReactive species:Unspecified reactive speciesK Pulford,K Micklem,S McCarthy,J Cordell,M Jones,D Y MasonPubMed 1592433
Key facts
- Isotype
IgG1
- Host species
Mouse
- Conjugation
PE
- Excitation/Emission
Ex: 480;565nm, Em: 578nm
- Storage buffer
pH: 7.2
Preservative: 0.09% Sodium azide- Form
Liquid
- Clonality
Monoclonal
Immunogen
- Cell preparation containing CD163 protein. The exact immunogen used to generate this antibody is proprietary information. Database link Q86VB7
Reactivity data
Select an application| Flow Cyt | |
|---|---|
| Human | Tested |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 5 µL for 106 Cells | Notes ab91357 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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Target data
Function
Acute phase-regulated receptor involved in clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages and may thereby protect tissues from free hemoglobin-mediated oxidative damage. May play a role in the uptake and recycling of iron, via endocytosis of hemoglobin/haptoglobin and subsequent breakdown of heme. Binds hemoglobin/haptoglobin complexes in a calcium-dependent and pH-dependent manner. Exhibits a higher affinity for complexes of hemoglobin and multimeric haptoglobin of HP*1F phenotype than for complexes of hemoglobin and dimeric haptoglobin of HP*1S phenotype. Induces a cascade of intracellular signals that involves tyrosine kinase-dependent calcium mobilization, inositol triphosphate production and secretion of IL6 and CSF1. Isoform 3 exhibits the higher capacity for ligand endocytosis and the more pronounced surface expression when expressed in cells.After shedding, the soluble form (sCD163) may play an anti-inflammatory role, and may be a valuable diagnostic parameter for monitoring macrophage activation in inflammatory conditions.
Alternative names
M130, M130, CD163, Scavenger receptor cysteine-rich type 1 protein M130, Hemoglobin scavenger receptor
Recommended products
Mouse Monoclonal CD163 antibody - conjugated to PE. Suitable for Flow Cyt and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Cell preparation containing CD163 protein.
Key facts
- Isotype
IgG1
- Host species
Mouse
- Conjugation
PE
- Excitation/Emission
Ex: 480;565nm, Em: 578nm
- Storage buffer
pH: 7.2
Preservative: 0.09% Sodium azide- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- Cell preparation containing CD163 protein. The exact immunogen used to generate this antibody is proprietary information. Database link Q86VB7
- Clone number
GHI/61
- Purification technique
Affinity purification Protein G
- Light chain type
kappa
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
+4°C
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
![Flow Cytometry - PE Anti-CD163 antibody [GHI/61] (ab95613), expandable thumbnail](https://content.abcam.com/products/images/pe-cd163-antibody-ghi-61-ab95613--flow-cytometry-img58643.jpg "Flow Cytometry - PE Anti-CD163 antibody [GHI/61] (ab95613)")
Flow Cytometry - PE Anti-CD163 antibody [GHI/61] (ab95613)
Staining of normal Human peripheral blood cells with Mouse IgG1 ? Isotype Control PE (open histogram) or ab95613 (filled histogram).
Cells in the monocyte gate were used for analysis.
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