AB69777

PE Anti-CD177 antibody [MEM-166]

Name: Anti-CD177 PE antibody [MEM-166] (ab69777) Mouse monoclonal IgG1 | Abcam
Brand: Abcam Limited
SKU: AB69777
Price: 525 USD
Availability: InStock

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(1 Publication)

Mouse Monoclonal CD177 antibody - conjugated to PE. Suitable for Flow Cyt and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Cell preparation containing CD177 protein.

View Alternative Names

CD177, NB1, PRV1, UNQ595/PRO1181, CD177 antigen, Human neutrophil alloantigen 2a, NB1 glycoprotein, Polycythemia rubra vera protein 1, HNA-2a, NB1 GP, PRV-1

2 Images

Flow Cytometry - PE Anti-CD177 antibody [MEM-166] (AB69777)

Flow Cyt

Unknown

Flow Cytometry - PE Anti-CD177 antibody [MEM-166] (AB69777)

Flow cytometry surface staining pattern of human peripheral whole blood stained using ab69777 (20 μl reagent / 100 μl of peripheral whole blood).

Flow Cyt

Unknown

Flow Cytometry - PE Anti-CD177 antibody [MEM-166] (AB69777)

Separation of human CD177 positive neutrophil granulocytes (red-filled) from lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using ab69777 (20 μl reagent / 100 μl of peripheral whole blood).

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

MEM-166

Isotype

IgG1

Conjugation

Excitation/Emission

Ex: 480;565nm, Em: 578nm

Carrier free

Reacts with

Human

Applications

Flow Cyt

applications

Immunogen

Cell preparation containing CD177 protein. The exact immunogen used to generate this antibody is proprietary information.

Q8N6Q3

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p>Use 20 μl per 100 μl of whole blood or 10<sup>6</sup> cells in a suspension</p>" }, "Primates": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Size-exclusion chromatography

Purification notes

The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions.

Storage buffer

pH: 7.4 Preservative: 0.097% Sodium azide Constituents: PBS, 0.2% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD177 also known as neutrophil antigen NB1 is a glycoprotein with an approximate mass of 58 kDa. It is primarily expressed on the surface of neutrophils a type of white blood cell involved in immune response. The expression of CD177 can vary among individuals where some people might have a higher or lower presence on their neutrophils. CD177 has been identified to play a role in cell adhesion and signaling functions contributing to various cellular processes within the immune system.

Biological function summary

CD177 functions by mediating interactions between neutrophils and endothelial cells during inflammation. This protein is not known to be part of a multi-protein complex but it plays a significant role in facilitating neutrophil transmigration from blood vessels to sites of tissue damage or infection. This process supports the body's defense mechanisms by enabling neutrophils to quickly respond to external threats and participate in the inflammatory response.

Pathways

The involvement of CD177 in chemotactic activity and cell migration highlights its contribution to both the immune response and inflammation pathways. Its interaction with proteins such as PECAM-1 (Platelet Endothelial Cell Adhesion Molecule-1) enhances neutrophil transmigration across the endothelium. CD177 acts within these pathways to ensure a precise and rapid movement of neutrophils which is essential for an effective immune response.

CD177 levels have associations with conditions like polycythemia vera and anti-neutrophil cytoplasmic antibody (ANCA)-associated vasculitis. In polycythemia vera CD177 can affect the proliferation of blood cells due to its role in neutrophil function. In ANCA-associated vasculitis CD177's interaction with proteins such as PR3 (Proteinase 3) on the surface of neutrophils could contribute to the pathophysiology of the disease leading to inappropriate activation of the immune system and subsequent tissue damage.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

In association with beta-2 integrin heterodimer ITGAM/CD11b and ITGB2/CD18, mediates activation of TNF-alpha primed neutrophils including degranulation and superoxide production (PubMed : 21193407). In addition, by preventing beta-2 integrin internalization and attenuating chemokine signaling favors adhesion over migration (PubMed : 28807980). Heterophilic interaction with PECAM1 on endothelial cells plays a role in neutrophil transendothelial migration in vitro (PubMed : 17580308). However, appears to be dispensable for neutrophil recruitment caused by bacterial infection in vivo (PubMed : 23461681). Acts as a receptor for the mature form of protease PRTN3 allowing its display at the cell surface of neutrophils (PubMed : 17244676, PubMed : 18462208). By displaying PRTN3 at the neutrophil cell surface, may play a role in enhancing endothelial cell junctional integrity and thus vascular integrity during neutrophil diapedesis (PubMed : 23202369).

See full target information CD177

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of leukocyte biology 97:181-9 PubMed25387833

2014

Olfactomedin-4 autoantibodies give unusual c-ANCA staining patterns with reactivity to a subpopulation of neutrophils.

Applications

Unspecified application

Species

Unspecified reactive species

Firoozeh Amirbeagi,Pontus Thulin,Rille Pullerits,Bo Pedersen,Bengt A Andersson,Claes Dahlgren,Amanda Welin,Johan Bylund

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

PE Anti-CD177 antibody [MEM-166]

Flow Cytometry - PE Anti-CD177 antibody [MEM-166] (AB69777)

Flow Cytometry - PE Anti-CD177 antibody [MEM-166] (AB69777)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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