AB1168

PE Anti-CD19 antibody [CB19]

Name: PE Anti-CD19 antibody [CB19] (ab1168) | Abcam
Brand: Abcam Limited
SKU: AB1168
Rating: 5 (1 reviews)

Lab Essentials
What is this?

(1 Review)

(5 Publications)

Anti-CD19 antibody [CB19] - PE conjugated (ab1168) is a mouse monoclonal antibody detecting CD19 in Flow Cytometry. Suitable for Chimpanzee, Human, Macaque monkey, Rhesus monkey.

- Trusted since 2002

View Alternative Names

CD19, B-lymphocyte antigen CD19, B-lymphocyte surface antigen B4, Differentiation antigen CD19, T-cell surface antigen Leu-12

1 Images

Flow Cytometry - PE Anti-CD19 antibody [CB19] (AB1168)

Flow Cyt

Supplier Data

Flow Cytometry - PE Anti-CD19 antibody [CB19] (AB1168)

Flow cytometry histogram showing 10µL of PE Anti-CD19 antibody [CB19] – (ab1168) used to stain 100µL of stabilized whole blood from healthy donor. A positive procedural control for T-lymphocyte, B-lymphocyte and NK cell enumeration (stabilized blood) was stained using mouse anti human CD19 RPE. Cells were gated on lymphocytes (using SSC/FSC dot plot). The first peak (near to the axis origin) shows negative cells (CD19-), the second peak shows the positive population, B cells according to the antibody specificity (CD19+).

Unconjugated

Anti-CD19 antibody [CB19]
660 APC

APC Anti-CD19 antibody [CB19]
519 FITC

FITC Anti-CD19 antibody [CB19]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

CB19

Isotype

IgG1

Light chain type

unknown

Conjugation

Excitation/Emission

Ex: 480;565nm, Em: 578nm

Carrier free

Reacts with

Human, Rhesus monkey, Macaque monkey, Chimpanzee

Applications

Flow Cyt

applications

Immunogen

Cell preparation containing CD19 protein. The exact immunogen used to generate this antibody is proprietary information.

P15391

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "Characterization of leukemias and lymphomas in human lysed whole peripheral blood or mononuclear cells separated by density gradient. CD19 (PE) immunofluorescence analysis can be performed on a flow cytometerequipped with an excitation source of 488nm and fitted with logarithmic amplifiers. 10ul of CD19 (PE) is sufficient for labelling of 1x10^6 cells. <a href='/en-us/products/primary-antibodies/pe-mouse-igg1-b11-6-isotype-control-ab91357'>ab91357</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody." }, "Chimpanzee": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "Characterization of leukemias and lymphomas in human lysed whole peripheral blood or mononuclear cells separated by density gradient. CD19 (PE) immunofluorescence analysis can be performed on a flow cytometerequipped with an excitation source of 488nm and fitted with logarithmic amplifiers. 10ul of CD19 (PE) is sufficient for labelling of 1x10^6 cells. <a href='/en-us/products/primary-antibodies/pe-mouse-igg1-b11-6-isotype-control-ab91357'>ab91357</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody." }, "Macaque monkey": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "Characterization of leukemias and lymphomas in human lysed whole peripheral blood or mononuclear cells separated by density gradient. CD19 (PE) immunofluorescence analysis can be performed on a flow cytometerequipped with an excitation source of 488nm and fitted with logarithmic amplifiers. 10ul of CD19 (PE) is sufficient for labelling of 1x10^6 cells. <a href='/en-us/products/primary-antibodies/pe-mouse-igg1-b11-6-isotype-control-ab91357'>ab91357</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody." }, "Rhesus monkey": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "Characterization of leukemias and lymphomas in human lysed whole peripheral blood or mononuclear cells separated by density gradient. CD19 (PE) immunofluorescence analysis can be performed on a flow cytometerequipped with an excitation source of 488nm and fitted with logarithmic amplifiers. 10ul of CD19 (PE) is sufficient for labelling of 1x10^6 cells. <a href='/en-us/products/primary-antibodies/pe-mouse-igg1-b11-6-isotype-control-ab91357'>ab91357</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody." } } }

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Product details

What is this antibody validated in?
PE Anti-CD19 antibody [CB19] (ab1168) is a mouse monoclonal antibody and is validated for use in Flow Cytometry (Flow Cyt) in Chimpanzee, Human, Macaque monkey, Rhesus monkey samples.

Related products
Antibody clone CB19 is also available pre-conjugated to a variety of labels for your convenience – Anti-CD19 PE [CB19] (ab1168).

Other related products
We have a range of other formats of antibody clone [CB19] also available for your convenience: ab254170, Carrier free - ab255925, Alexa Fluor® 647 - ab307266, FITC - ab1167, PE - ab1168

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Properties and storage information

Form

Liquid

Storage buffer

Preservative: 0.1% Sodium azide Constituents: 0.5% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD19 is a transmembrane protein also known as B4 or B-lymphocyte antigen CD19 with an approximate molecular weight of 95 kDa. It is expressed on the surface of B cells throughout their development from pro-B cells to mature B cells. CD19 functions mechanically as a coreceptor enhancing the sensitivity of B cell receptor (BCR) signaling. This protein plays a significant role in promoting the activation and proliferation of B cells by lowering the threshold for antigen receptor engagement.

Biological function summary

CD19 acts as an essential player in B cell signaling. CD19 integrates signals from the BCR with other coreceptors acting as a signaling hub. It participates in forming a signaling complex that includes proteins like CD21 and CD81 which further amplifies BCR-mediated signaling. This complex plays a critical role in B cell activation differentiation and survival ensuring that immune responses are efficiently mounted against pathogens.

Pathways

The CD19 protein fits into key immune response pathways such as the BCR signaling pathway and the PI3K-Akt signaling pathway. CD19 collaborates with other proteins like CD21 CD81 and PI3K within these pathways. Its interaction in the PI3K-Akt pathway for instance supports critical processes including B cell activation and homeostasis contributing to the adaptive immune response.

CD19 is connected to B cell malignancies and autoimmune diseases. B cell acute lymphoblastic leukemia (ALL) often shows aberrant CD19 expression making it a valuable target for therapeutic interventions like anti-CD19 monoclonal antibodies. Additionally overexpression or mutations of CD19 may contribute to autoimmune diseases by disrupting normal B cell regulation. CD19's connection with proteins like CD22 and CD20 in these pathological contexts highlights its relevance in developing targeted therapies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Functions as a coreceptor for the B-cell antigen receptor complex (BCR) on B-lymphocytes (PubMed : 29523808). Decreases the threshold for activation of downstream signaling pathways and for triggering B-cell responses to antigens (PubMed : 1373518, PubMed : 16672701, PubMed : 2463100). Activates signaling pathways that lead to the activation of phosphatidylinositol 3-kinase and the mobilization of intracellular Ca(2+) stores (PubMed : 12387743, PubMed : 16672701, PubMed : 9317126, PubMed : 9382888). Is not required for early steps during B cell differentiation in the blood marrow (PubMed : 9317126). Required for normal differentiation of B-1 cells (By similarity). Required for normal B cell differentiation and proliferation in response to antigen challenges (PubMed : 1373518, PubMed : 2463100). Required for normal levels of serum immunoglobulins, and for production of high-affinity antibodies in response to antigen challenge (PubMed : 12387743, PubMed : 16672701, PubMed : 9317126).

See full target information CD19

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 15:5037 PubMed39934157

2025

The antidiabetic effect of safflower yellow by regulating the GOAT/ghrelin/GHS-R1a/cAMP/TRPM2 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yunxiao Ma,Haifeng Zhang,Qihui Yan,Ping Wang,Weiying Guo,Lu Yu

Circulation 138:1144-1154 PubMed29593014

2018

Ryanodine Receptor Calcium Leak in Circulating B-Lymphocytes as a Biomarker in Heart Failure.

Applications

Unspecified application

Species

Unspecified reactive species

Alexander Kushnir,Gaetano Santulli,Steven R Reiken,Ellie Coromilas,Sarah J Godfrey,Danielle L Brunjes,Paolo C Colombo,Melana Yuzefpolskaya,Seth I Sokol,Richard N Kitsis,Andrew R Marks

Nature communications 8:14930 PubMed28374737

2017

Extinct type of human parvovirus B19 persists in tonsillar B cells.

Applications

Flow Cyt

Species

Unspecified reactive species

Lari Pyöriä,Mari Toppinen,Elina Mäntylä,Lea Hedman,Leena-Maija Aaltonen,Maija Vihinen-Ranta,Taru Ilmarinen,Maria Söderlund-Venermo,Klaus Hedman,Maria F Perdomo

Journal of immunology (Baltimore, Md. : 1950) 149:2841-50 PubMed1383329

1992

The CD19/CD21 signal transducing complex of human B lymphocytes includes the target of antiproliferative antibody-1 and Leu-13 molecules.

Applications

Unspecified application

Species

Unspecified reactive species

L E Bradbury,G S Kansas,S Levy,R L Evans,T F Tedder

Science (New York, N.Y.) 256:105-7 PubMed1373518

1992

CD19: lowering the threshold for antigen receptor stimulation of B lymphocytes.

Applications

Unspecified application

Species

Unspecified reactive species

R H Carter,D T Fearon

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

PE Anti-CD19 antibody [CB19]

Flow Cytometry - PE Anti-CD19 antibody [CB19] (AB1168)

Related conjugates and formulations (3)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

Product promise