Anti-CD19 [CB19] PE conjugated antibody (ab1168) mouse monoclonal antibody that is used to detect CD19 in Flow Cytometry. Suitable for Chimpanzee, Human, Macaque monkey, Rhesus monkey samples.
- Trusted since 2002
Preservative: 0.1% Sodium azide
Constituents: 0.5% BSA
| Flow Cyt | |
|---|---|
| Human | Expected |
| Chimpanzee | Expected |
| Macaque monkey | Expected |
| Rhesus monkey | Expected |
| Species | Dilution info | Notes |
|---|---|---|
Species Macaque monkey | Dilution info - | Notes Characterization of leukemias and lymphomas in human lysed whole peripheral blood or mononuclear cells separated by density gradient. CD19 (PE) immunofluorescence analysis can be performed on a flow cytometerequipped with an excitation source of 488nm and fitted with logarithmic amplifiers. 10ul of CD19 (PE) is sufficient for labelling of 1x10^6 cells. ab91357 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Species Rhesus monkey | Dilution info - | Notes Characterization of leukemias and lymphomas in human lysed whole peripheral blood or mononuclear cells separated by density gradient. CD19 (PE) immunofluorescence analysis can be performed on a flow cytometerequipped with an excitation source of 488nm and fitted with logarithmic amplifiers. 10ul of CD19 (PE) is sufficient for labelling of 1x10^6 cells. ab91357 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Species Chimpanzee | Dilution info - | Notes Characterization of leukemias and lymphomas in human lysed whole peripheral blood or mononuclear cells separated by density gradient. CD19 (PE) immunofluorescence analysis can be performed on a flow cytometerequipped with an excitation source of 488nm and fitted with logarithmic amplifiers. 10ul of CD19 (PE) is sufficient for labelling of 1x10^6 cells. ab91357 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Species Human | Dilution info - | Notes Characterization of leukemias and lymphomas in human lysed whole peripheral blood or mononuclear cells separated by density gradient. CD19 (PE) immunofluorescence analysis can be performed on a flow cytometerequipped with an excitation source of 488nm and fitted with logarithmic amplifiers. 10ul of CD19 (PE) is sufficient for labelling of 1x10^6 cells. ab91357 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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Functions as a coreceptor for the B-cell antigen receptor complex (BCR) on B-lymphocytes (PubMed:29523808). Decreases the threshold for activation of downstream signaling pathways and for triggering B-cell responses to antigens (PubMed:1373518, PubMed:16672701, PubMed:2463100). Activates signaling pathways that lead to the activation of phosphatidylinositol 3-kinase and the mobilization of intracellular Ca(2+) stores (PubMed:12387743, PubMed:16672701, PubMed:9317126, PubMed:9382888). Is not required for early steps during B cell differentiation in the blood marrow (PubMed:9317126). Required for normal differentiation of B-1 cells (By similarity). Required for normal B cell differentiation and proliferation in response to antigen challenges (PubMed:1373518, PubMed:2463100). Required for normal levels of serum immunoglobulins, and for production of high-affinity antibodies in response to antigen challenge (PubMed:12387743, PubMed:16672701, PubMed:9317126).
CD19, B-lymphocyte antigen CD19, B-lymphocyte surface antigen B4, Differentiation antigen CD19, T-cell surface antigen Leu-12
Anti-CD19 [CB19] PE conjugated antibody (ab1168) mouse monoclonal antibody that is used to detect CD19 in Flow Cytometry. Suitable for Chimpanzee, Human, Macaque monkey, Rhesus monkey samples.
- Trusted since 2002
Preservative: 0.1% Sodium azide
Constituents: 0.5% BSA
CD19 is a transmembrane protein also known as B4 or B-lymphocyte antigen CD19 with an approximate molecular weight of 95 kDa. It is expressed on the surface of B cells throughout their development from pro-B cells to mature B cells. CD19 functions mechanically as a coreceptor enhancing the sensitivity of B cell receptor (BCR) signaling. This protein plays a significant role in promoting the activation and proliferation of B cells by lowering the threshold for antigen receptor engagement.
CD19 acts as an essential player in B cell signaling. CD19 integrates signals from the BCR with other coreceptors acting as a signaling hub. It participates in forming a signaling complex that includes proteins like CD21 and CD81 which further amplifies BCR-mediated signaling. This complex plays a critical role in B cell activation differentiation and survival ensuring that immune responses are efficiently mounted against pathogens.
The CD19 protein fits into key immune response pathways such as the BCR signaling pathway and the PI3K-Akt signaling pathway. CD19 collaborates with other proteins like CD21 CD81 and PI3K within these pathways. Its interaction in the PI3K-Akt pathway for instance supports critical processes including B cell activation and homeostasis contributing to the adaptive immune response.
CD19 is connected to B cell malignancies and autoimmune diseases. B cell acute lymphoblastic leukemia (ALL) often shows aberrant CD19 expression making it a valuable target for therapeutic interventions like anti-CD19 monoclonal antibodies. Additionally overexpression or mutations of CD19 may contribute to autoimmune diseases by disrupting normal B cell regulation. CD19's connection with proteins like CD22 and CD20 in these pathological contexts highlights its relevance in developing targeted therapies.
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This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
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