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AB326505

PE Anti-CD1A antibody [HI149]

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Mouse Monoclonal CD1A antibody - conjugated to PE. Suitable for Flow Cyt and reacts with Human samples.

View Alternative Names

CD1a, T-cell surface glycoprotein CD1a, T-cell surface antigen T6/Leu-6, hTa1 thymocyte antigen, CD1A

7 Images
Flow Cytometry - PE Anti-CD1A antibody [HI149] (AB326505)
  • Flow Cyt

Supplier Data

Flow Cytometry - PE Anti-CD1A antibody [HI149] (AB326505)

Flow cytometry surface staining pattern of human stimulated (GM-CSF + IL-4) peripheral blood monocytes stained using ab326505 (20 μl reagent per million cells in 100 μl of cell suspension).

Flow Cytometry - PE Anti-CD1A antibody [HI149] (AB326505)
  • Flow Cyt

Supplier Data

Flow Cytometry - PE Anti-CD1A antibody [HI149] (AB326505)

Expression profiling on peripheral blood subsets using ab326505. Innate panel

HCDM CDMaps standardized procedures (Kuzilkova D et al. Front Immunol. 2022;13 : 827898) were used for cell isolation and surface staining of blood leukocytes, with the modification of staining protocol using cytometry test tubes.
Suspension of blood leukocytes isolated from buffy coats (2 x 10^6 cells) was added to the mixture of ab326505 (1.6 µg/ml in stained blood sample) and Monocyte Blocking Buffer, vortexed and incubated for 20 min. Next, optimized backbone antibody panel (HLDA Innate) was added to test tubes, vortexed and incubated for 20 min. The residual erythrocytes were lysed with 2 ml of 10Ч diluted EXCELLYSE Easy solution and incubated for 10 min. Finally, samples were centrifuged (670 g, 5 min.), supernatant removed and the cell pellet was resuspended in 200 µl of PBS for acquisition.

Flow Cytometry - PE Anti-CD1A antibody [HI149] (AB326505)
  • Flow Cyt

Supplier Data

Flow Cytometry - PE Anti-CD1A antibody [HI149] (AB326505)

Flow cytometry multicolor surface staining of human stimulated (GM-CSF + IL-4) peripheral blood monocytes stained using ab326505 (20 μl reagent per million cells in 100 μl of cell suspension) and anti-human CD11c (BU15) APC antibody (10 μl reagent per million cells in 100 μl of cell suspension).

Flow Cytometry - PE Anti-CD1A antibody [HI149] (AB326505)
  • Flow Cyt

Supplier Data

Flow Cytometry - PE Anti-CD1A antibody [HI149] (AB326505)

Separation of human CD1a positive CD11c positive dendritic cells differentiated upon monocyte stimulation (GM-CSF + IL-4) (red-filled) from CD11c negative CD1a negative events (black-dashed) in flow cytometry analysis (surface staining) of human stimulated (GM-CSF + IL-4) peripheral blood monocytes stained using ab326505 (20 μl reagent per million cells in 100 μl of cell suspension).

Flow Cytometry - PE Anti-CD1A antibody [HI149] (AB326505)
  • Flow Cyt

Supplier Data

Flow Cytometry - PE Anti-CD1A antibody [HI149] (AB326505)

Expression profiling on peripheral blood subsets using ab326505. Adaptive panel

HCDM CDMaps standardized procedures (Kuzilkova D et al. Front Immunol. 2022;13 : 827898) were used for cell isolation and surface staining of blood leukocytes, with the modification of staining protocol using cytometry test tubes.
Suspension of blood leukocytes isolated from buffy coats (2 x 10^6 cells) was added to the mixture ofab326505 (1.6 µg/ml in stained blood sample) and Monocyte Blocking Buffer, vortexed and incubated for 20 min. Next, optimized backbone antibody panel (HLDA Adaptive) was added to test tubes, vortexed and incubated for 20 min. The residual erythrocytes were lysed with 2 ml of 10Ч diluted EXCELLYSE Easy solution and incubated for 10 min. Finally, samples were centrifuged (670 g, 5 min.), supernatant removed and the cell pellet was resuspended in 200 µl of PBS for acquisition.

Flow Cytometry - PE Anti-CD1A antibody [HI149] (AB326505)
  • Flow Cyt

Supplier Data

Flow Cytometry - PE Anti-CD1A antibody [HI149] (AB326505)

Analysis of the antibody staining profile was performed on blood leukocytes isolated from buffy coats. HCDM CDMaps standardized procedures (Kuzilkova D et al. Front Immunol. 2022;13 : 827898) were used for cell isolation and surface staining of blood leukocytes, with the modification of staining protocol using cytometry test tubes.
ab326505 was used in concentration 1.6 µg/ml in stained blood sample (2 x 10^6 cells).

Flow Cytometry - PE Anti-CD1A antibody [HI149] (AB326505)
  • Flow Cyt

Supplier Data

Flow Cytometry - PE Anti-CD1A antibody [HI149] (AB326505)

Separation of MOLT-4 cells stained using ab326505 (concentration in sample 4 μg/m, red-filled) from MOLT-4 cells stained using mouse IgG1 isotype control (MOPC-21) PE antibody (concentration in sample 4 μg/ml, same as CD1a PE concentration, black-dashed) in flow cytometry analysis (surface staining).

  • 660 APC

    APC Anti-CD1A antibody [HI149]

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    FITC Anti-CD1a antibody [HI149]

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    mFluor™ UV375 Anti-CD1a antibody [HI149]

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    mFluor™ Violet 450 Anti-CD1a antibody [HI149]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

HI149

Isotype

IgG1

Conjugation

PE

Excitation/Emission

Ex: 480;565nm, Em: 578nm

Carrier free

No

Reacts with

Human

Applications

Flow Cyt

applications

Specificity

The antibody HI149 reacts with an extracellular epitope of CD1a (T6), a 49 kDa polypeptide associated with beta2-microglobulin expressed on cortical thymocytes (strongly), Langerhans cells, dendritic cells and some T cell leukemias and lymphomas. The antibody does not react with peripheral blood T and B lymphocytes, monocytes, granulocytes, platelets and erythrocytes.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p>Flow cytometry: The reagent is designed for analysis of human blood cells using 20 μl reagent / 100 μl of whole blood or 10^6 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Size-exclusion chromatography
Purification notes
Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Storage buffer
pH: 7.4 Preservative: 0.098% Sodium azide Constituents: PBS, 0.2% BSA
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze|Store in the dark
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Antigen-presenting protein that binds self and non-self lipid and glycolipid antigens and presents them to T-cell receptors on natural killer T-cells.
See full target information CD1A
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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