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AB313954

PE Anti-CD1c antibody [EPR23189-305]

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Rabbit Recombinant Monoclonal CD1C antibody - conjugated to PE. Suitable for Flow Cyt and reacts with Human samples.

View Alternative Names

CD1c, T-cell surface glycoprotein CD1c, CD1C

2 Images
Flow Cytometry - PE Anti-CD1c antibody [EPR23189-305] (AB313954)
  • Flow Cyt

Lab

Flow Cytometry - PE Anti-CD1c antibody [EPR23189-305] (AB313954)

Flow cytometry overlay histogram showing wild-type Jurkat (green line) and CD1C knockout Jurkat stained with ab313954 (red line). The cells were incubated in 1x PBS containing 10% normal goat serum to block non-specific protein-protein interaction followed by the antibody (ab313954) (1x 106 in 100 μl at 0.04 μg/ml (1/12500)) for 30 min on ice.

Isotype control antibody Rabbit IgG (monoclonal) Phycoerythrin Isotype Control (ab209478) was used at the same concentration and conditions as the primary antibody (wild-type Jurkat - black line, CD1C knockout Jurkat - grey line). Unlabelled sample was also used as a control (this line is not shown for the purpose of simplicity).

Acquisition of >5000 events were collected using a 50 mW Yellow/Green laser (561nm) and 585/42 bandpass filter.

Flow Cytometry - PE Anti-CD1c antibody [EPR23189-305] (AB313954)
  • Flow Cyt

Lab

Flow Cytometry - PE Anti-CD1c antibody [EPR23189-305] (AB313954)

Flow cytometry overlay histogram showing left Jurkat positive cells and right negative THP-1 stained with ab313954 (red line). The cells were incubated in 1x PBS containing 10 μg/ml human IgG and 10% normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody (ab313954) (1x 106 in 100 μl at 0.2 μg/ml (1/2500)) for 30 min on ice.

Isotype control antibody (black line) was Rabbit IgG (monoclonal) Phycoerythrin Isotype Control (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

Acquisition of >5000 events were collected using a 50 mW Yellow/Green laser (561nm) and 585/42 bandpass filter.

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-CD1c antibody [EPR23189-305]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-CD1c antibody [EPR23189-305]

  • 660 APC

    APC Anti-CD1c antibody [EPR23189-305]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR23189-305

Isotype

IgG

Conjugation

PE

Excitation/Emission

Ex: 480;565nm, Em: 578nm

Carrier free

No

Reacts with

Human

Applications

Flow Cyt

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/2500 - 1/12500", "FlowCyt-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD1c also known as CD1c molecule or R7 is a transmembrane glycoprotein with a molecular weight of approximately 43 kDa. It belongs to the CD1 family of glycoproteins which share structural similarity to major histocompatibility complex (MHC) proteins. CD1c presents lipid and glycolipid antigens to T cells. It is expressed on professional antigen-presenting cells (APCs) like dendritic cells B cells and some monocytes. This expression allows CD1c to function in the immune response by presenting specialized antigens to T cells.
Biological function summary

The function of CD1c involves the presentation of lipid antigens which plays a role in immune recognition. CD1c is a part of a complex that includes β2-microglobulin which stabilizes its structure for antigen presentation. CD1c contributes to the immune surveillance by enabling the recognition of microbial lipid antigens as well as self-lipids by specific T cell subsets. This function supports the body's immune defense by identifying and reacting to potential pathogenic lipids.

Pathways

CD1c is involved in immune response pathways that center around antigen presentation. It interacts with other proteins like CD1a CD1b and CD1d expanding the range of antigens that T cells can recognize. CD1c participates in the lipid antigen presentation pathway influencing both innate and adaptive immunity. The presentation of lipid antigens by CD1c to T cells can trigger signaling pathways that affect both immune activation and resolution.

CD1c is implicated in autoimmune diseases and infections. Its role in antigen presentation makes it a potential factor in autoimmune conditions where immune recognition errors might occur. Additionally CD1c may relate to infectious diseases where mycobacteria or other pathogens present unique lipid antigens. Through its interaction with T cell receptors CD1c can impact how well immune responses are mounted against these challenges making it a notable target for therapeutic development and research.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Antigen-presenting protein that binds self and non-self lipid and glycolipid antigens and presents them to T-cell receptors on natural killer T-cells.
See full target information CD1C
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com