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AB326524

PE Anti-CD1c antibody [L161]

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Mouse Monoclonal CD1C antibody - conjugated to PE. Suitable for Flow Cyt and reacts with Human samples.

View Alternative Names

CD1c, T-cell surface glycoprotein CD1c, CD1C

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Flow Cytometry - PE Anti-CD1c antibody [L161] (AB326524)
  • Flow Cyt

Supplier Data

Flow Cytometry - PE Anti-CD1c antibody [L161] (AB326524)

Expression profiling on peripheral blood subsets using ab326524. Adaptive panel

HCDM CDMaps standardized procedures (Kuzilkova D et al. Front Immunol. 2022;13 : 827898) were used for cell isolation and surface staining of blood leukocytes, with the modification of staining protocol using cytometry test tubes.
Suspension of blood leukocytes isolated from buffy coats (2 x 10^6 cells) was added to the mixture of ab326524 (1 µg/ml in stained blood sample) and Monocyte Blocking Buffer, vortexed and incubated for 20 min. Next, optimized backbone antibody panel (HLDA Adaptive) was added to test tubes, vortexed and incubated for 20 min. The residual erythrocytes were lysed with 2 ml of 10X diluted EXCELLYSE Easy solution and incubated for 10 min. Finally, samples were centrifuged (670 g, 5 min.), supernatant removed and the cell pellet was resuspended in 200 µl of PBS for acquisition.

Flow Cytometry - PE Anti-CD1c antibody [L161] (AB326524)
  • Flow Cyt

Supplier Data

Flow Cytometry - PE Anti-CD1c antibody [L161] (AB326524)

Expression profiling on peripheral blood subsets using ab326524. Innate panel

HCDM CDMaps standardized procedures (Kuzilkova D et al. Front Immunol. 2022;13 : 827898) were used for cell isolation and surface staining of blood leukocytes, with the modification of staining protocol using cytometry test tubes.
Suspension of blood leukocytes isolated from buffy coats (2 x 10^6 cells) was added to the mixture of ab326524 (1 µg/ml in stained blood sample) and Monocyte Blocking Buffer, vortexed and incubated for 20 min. Next, optimized backbone antibody panel (HLDA Innate) was added to test tubes, vortexed and incubated for 20 min. The residual erythrocytes were lysed with 2 ml of 10X diluted EXCELLYSE Easy solution and incubated for 10 min. Finally, samples were centrifuged (670 g, 5 min.), supernatant removed and the cell pellet was resuspended in 200 µl of PBS for acquisition.

Flow Cytometry - PE Anti-CD1c antibody [L161] (AB326524)
  • Flow Cyt

Supplier Data

Flow Cytometry - PE Anti-CD1c antibody [L161] (AB326524)

Analysis of the antibody staining profile was performed on blood leukocytes isolated from buffy coats. HCDM CDMaps standardized procedures (Kuzilkova D et al. Front Immunol. 2022;13 : 827898) were used for cell isolation and surface staining of blood leukocytes, with the modification of staining protocol using cytometry test tubes.
ab326524 was used in concentration 1 µg/ml in stained blood sample (2 x 10^6 cells).

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

L161

Isotype

IgG1

Light chain type

kappa

Conjugation

PE

Excitation/Emission

Ex: 480;565nm, Em: 578nm

Carrier free

No

Reacts with

Human

Applications

Flow Cyt

applications

Specificity

The mouse monoclonal antibody L161 recognizes an extracellular epitope of CD1c, (R7), a 43 kDa type I glycoprotein associated with beta2-microglobulin. It is expressed on cortical thymocytes (strongly), Langerhans cells, dendritic cells, B and some T cells.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p>Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 10^6 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Size-exclusion chromatography
Purification notes
Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Storage buffer
pH: 7.4 Preservative: 0.098% Sodium azide Constituents: PBS, 0.2% BSA
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze|Store in the dark
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Antigen-presenting protein that binds self and non-self lipid and glycolipid antigens and presents them to T-cell receptors on natural killer T-cells.
See full target information CD1C
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Product promise

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For full details, please see our Terms & Conditions

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