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AB326514

PE Anti-CD20 antibody [2H7]

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Mouse Monoclonal CD20 antibody - conjugated to PE. Suitable for Flow Cyt and reacts with Human samples.

View Alternative Names

CD20, MS4A1, B-lymphocyte antigen CD20, B-lymphocyte surface antigen B1, Bp35, Leukocyte surface antigen Leu-16, Membrane-spanning 4-domains subfamily A member 1

3 Images
Flow Cytometry - PE Anti-CD20 antibody [2H7] (AB326514)
  • Flow Cyt

Supplier Data

Flow Cytometry - PE Anti-CD20 antibody [2H7] (AB326514)

Expression profiling on peripheral blood subsets using ab326514. Innate panel

HCDM CDMaps standardized procedures (Kuzilkova D et al. Front Immunol. 2022;13 : 827898) were used for cell isolation and surface staining of blood leukocytes, with the modification of staining protocol using cytometry test tubes.
Suspension of blood leukocytes isolated from buffy coats (2 x 10^6 cells) was added to the mixture of ab326514 (2 µg/ml in stained blood sample) and Monocyte Blocking Buffer, vortexed and incubated for 20 min. Next, optimized backbone antibody panel (HLDA Innate) was added to test tubes, vortexed and incubated for 20 min. The residual erythrocytes were lysed with 2 ml of 10Ч diluted EXCELLYSE Easy solution and incubated for 10 min. Finally, samples were centrifuged (670 g, 5 min.), supernatant removed and the cell pellet was resuspended in 200 µl of PBS for acquisition.

Flow Cytometry - PE Anti-CD20 antibody [2H7] (AB326514)
  • Flow Cyt

Supplier Data

Flow Cytometry - PE Anti-CD20 antibody [2H7] (AB326514)

ab326514 works in flow cytometry application.

Analysis of the antibody staining profile was performed on blood leukocytes isolated from buffy coats. HCDM CDMaps standardized procedures (Kuzilkova D et al. Front Immunol. 2022;13 : 827898) were used for cell isolation and surface staining of blood leukocytes, with the modification of staining protocol using cytometry test tubes.
ab326514 was used in concentration 2 µg/ml in stained blood sample (2 x 10^6 cells).

Flow Cytometry - PE Anti-CD20 antibody [2H7] (AB326514)
  • Flow Cyt

Supplier Data

Flow Cytometry - PE Anti-CD20 antibody [2H7] (AB326514)

Expression profiling on peripheral blood subsets using ab326514. Adaptive panel

HCDM CDMaps standardized procedures (Kuzilkova D et al. Front Immunol. 2022;13 : 827898) were used for cell isolation and surface staining of blood leukocytes, with the modification of staining protocol using cytometry test tubes.
Suspension of blood leukocytes isolated from buffy coats (2 x 10^6 cells) was added to the mixture of ab326514 (2 µg/ml in stained blood sample) and Monocyte Blocking Buffer, vortexed and incubated for 20 min. Next, optimized backbone antibody panel (HLDA Adaptive) was added to test tubes, vortexed and incubated for 20 min. The residual erythrocytes were lysed with 2 ml of 10Ч diluted EXCELLYSE Easy solution and incubated for 10 min. Finally, samples were centrifuged (670 g, 5 min.), supernatant removed and the cell pellet was resuspended in 200 µl of PBS for acquisition.

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Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

2H7

Isotype

IgG2b

Light chain type

kappa

Conjugation

PE

Excitation/Emission

Ex: 480;565nm, Em: 578nm

Carrier free

No

Reacts with

Human

Applications

Flow Cyt

applications

Specificity

The mouse monoclonal antibody 2H7 recognizes an extracellular epitope on CD20 (B1, Bp35), a 33-37 kDa non-glycosylated membrane receptor with four transmembrane domains, expressed on pre-B lymphocytes, resting and activated B cells (not plasma cells), follicular dendritic cells, and at low levels on peripheral blood T lymphocytes.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p>Flow cytometry: The reagent is designed for analysis of human blood cells using 20 μl reagent / 100 μl of whole blood or 10^6 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Size-exclusion chromatography
Purification notes
Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Storage buffer
pH: 7.4 Preservative: 0.098% Sodium azide Constituents: PBS, 0.2% BSA
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze|Store in the dark
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

B-lymphocyte-specific membrane protein that plays a role in the regulation of cellular calcium influx necessary for the development, differentiation, and activation of B-lymphocytes (PubMed : 12920111, PubMed : 3925015, PubMed : 7684739). Functions as a store-operated calcium (SOC) channel component promoting calcium influx after activation by the B-cell receptor/BCR (PubMed : 12920111, PubMed : 18474602, PubMed : 7684739).
See full target information MS4A1
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

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