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Mouse Monoclonal CD22 antibody - conjugated to PE. Suitable for Flow Cyt, Flow Cyt (Intra) and reacts with Human samples. Immunogen corresponding to Cell preparation containing CD22 protein.

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Images

Flow Cytometry - PE Anti-CD22 antibody [S-HCL-1] (AB234253), expandable thumbnail
  • Flow Cytometry - PE Anti-CD22 antibody [S-HCL-1] (AB234253), expandable thumbnail

Key facts

Isotype
IgG2b
Host species
Mouse
Conjugation
PE
Excitation/Emission
Ex: 480;565nm, Em: 578nm
Storage buffer

pH: 7.4
Preservative: 0.0975% Sodium azide
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • Cell preparation containing CD22 protein. The exact immunogen used to generate this antibody is proprietary information. Database link P20273

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow CytFlow Cyt (Intra)
Human
Tested
Expected

Tested
Tested

Species
Human
Dilution info
10 µL for 106 Cells
Notes

(or 100 μl of whole blood).

Expected
Expected

Species
Human
Dilution info
10 µL for 106 Cells
Notes

(or 100 μl of whole blood).

Associated Products

Select an associated product type

3 products for Alternative Version

7 products for Alternative Product

Target data

Function

Most highly expressed siglec (sialic acid-binding immunoglobulin-like lectin) on B-cells that plays a role in various aspects of B-cell biology including differentiation, antigen presentation, and trafficking to bone marrow (PubMed:34330755, PubMed:8627166). Binds to alpha 2,6-linked sialic acid residues of surface molecules such as CD22 itself, CD45 and IgM in a cis configuration. Can also bind to ligands on other cells as an adhesion molecule in a trans configuration (PubMed:20172905). Acts as an inhibitory coreceptor on the surface of B-cells and inhibits B-cell receptor induced signaling, characterized by inhibition of the calcium mobilization and cellular activation. Mechanistically, the immunoreceptor tyrosine-based inhibitory motif domain is phosphorylated by the Src kinase LYN, which in turn leads to the recruitment of the protein tyrosine phosphatase 1/PTPN6, leading to the negative regulation of BCR signaling (PubMed:8627166). If this negative signaling from is of sufficient strength, apoptosis of the B-cell can be induced (PubMed:20516366).

Alternative names

CD22, SIGLEC2, B-cell receptor CD22, B-lymphocyte cell adhesion molecule, Sialic acid-binding Ig-like lectin 2, T-cell surface antigen Leu-14, BL-CAM, Siglec-2

Recommended products

Mouse Monoclonal CD22 antibody - conjugated to PE. Suitable for Flow Cyt, Flow Cyt (Intra) and reacts with Human samples. Immunogen corresponding to Cell preparation containing CD22 protein.

Key facts

Isotype
IgG2b
Conjugation
PE
Excitation/Emission
Ex: 480;565nm, Em: 578nm
Form
Liquid
Clonality
Monoclonal
Immunogen
  • Cell preparation containing CD22 protein. The exact immunogen used to generate this antibody is proprietary information. Database link P20273
Clone number
S-HCL-1
Purification technique
Size-exclusion chromatography
Concentration
Loading...
Purification notes

Purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Store in the dark

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

CD22 also known as Siglec-2 or B-lymphocyte cell adhesion molecule (BL-CAM) is a transmembrane protein with a mass of around 140 kDa. It is selectively expressed on the surface of mature B cells and some immature B cells. This protein plays an important role in the immune system particularly in the regulation of B cell function. CD22 functions by modulating signal transduction processes through its cytoplasmic domain which contains immunoreceptor tyrosine-based inhibitory motifs (ITIMs).

Biological function summary

CD22 acts mainly as a negative regulator of B cell receptor (BCR) signaling. It is part of a complex that interacts with the BCR and other accessory molecules. CD22 helps maintain B cell tolerance by inhibiting overactive signaling and preventing autoimmune responses. By binding to sialic acid-containing glycans CD22 modulates interactions between B cells and other cells aiding in the maintenance of immune homeostasis.

Pathways

CD22 plays a critical role in the BCR signaling pathway and the regulation of phosphatidylinositol 3-kinase (PI3K) signaling. It works together with proteins like CD19 and SHP-1 that are key components of these pathways. By influencing these signaling cascades CD22 contributes to the modulation of B cell activation proliferation and apoptosis ensuring proper functioning of the immune response.

Associated diseases and disorders

CD22 is associated with autoimmune conditions and hematological malignancies such as hairy cell leukemia (HCL). Therapeutic approaches targeting CD22 such as anti-CD22 antibodies are being explored for treating these diseases. CD22's role in these conditions is connected with proteins involved in the autoimmune pathways or cancer development offering potential therapeutic targets for intervention.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Flow Cytometry - PE Anti-CD22 antibody [S-HCL-1] (ab234253), expandable thumbnail

    Flow Cytometry - PE Anti-CD22 antibody [S-HCL-1] (ab234253)

    Flow cytometric analysis of human peripheral blood labeling CD22 with ab234253. Gated on leukocytes, surface staining.

  • Flow Cytometry - PE Anti-CD22 antibody [S-HCL-1] (ab234253), expandable thumbnail

    Flow Cytometry - PE Anti-CD22 antibody [S-HCL-1] (ab234253)

    Flow cytometric analysis of human peripheral whole blood showing separation of human CD22 positive lymphocytes (red-filled) from human neutrophil granulocytes (black-dashed) (surface staining) using anti-human CD22 PE antibody ab234253 (10 μl / 100 μl of peripheral whole blood).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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