Mouse Monoclonal CD226 antibody - conjugated to PE. Suitable for Flow Cyt and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Cell preparation containing CD226 protein.
Images
![Flow Cytometry - PE Anti-CD226 antibody [DX11] (AB33337), expandable thumbnail](https://content.abcam.com/products/images/pe-cd226-antibody-dx11-ab33337--flow-cytometry-img47634.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - PE Anti-CD226 antibody [DX11] (AB33337)")
Publications
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- The Journal of biological chemistry 278:36748-532003CD226 mediates platelet and megakaryocytic cell adhesion to vascular endothelial cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHiroshi Kojima et. al.PubMed 12847109
Key facts
- Isotype
IgG1
- Host species
Mouse
- Conjugation
PE
- Excitation/Emission
Ex: 480;565nm, Em: 578nm
- Storage buffer
pH: 7.4
Preservative: 0.09% Sodium azide
Constituents: PBS, 1% BSA- Form
Liquid
- Clonality
Monoclonal
Immunogen
- Cell preparation containing CD226 protein. The exact immunogen used to generate this antibody is proprietary information. Database link Q15762
Reactivity data
Select an application| Flow Cyt | |
|---|---|
| Human | Tested |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1 | Notes Use 10μl neat antibody to label 1 x 106 cells in 100μl. ab91357 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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2 products for Alternative Version
Target data
Function
Involved in intercellular adhesion, lymphocyte signaling, cytotoxicity and lymphokine secretion mediated by cytotoxic T-lymphocyte (CTL) and NK cell (PubMed:8673704). Cell surface receptor for NECTIN2. Upon ligand binding, stimulates T-cell proliferation and cytokine production, including that of IL2, IL5, IL10, IL13, and IFNG. Competes with PVRIG for NECTIN2-binding (PubMed:26755705).
Alternative names
CD226 antigen, DNAX accessory molecule 1, DNAM-1, CD226, DNAM1
Recommended products
Mouse Monoclonal CD226 antibody - conjugated to PE. Suitable for Flow Cyt and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Cell preparation containing CD226 protein.
Key facts
- Isotype
IgG1
- Host species
Mouse
- Conjugation
PE
- Excitation/Emission
Ex: 480;565nm, Em: 578nm
- Storage buffer
pH: 7.4
Preservative: 0.09% Sodium azide
Constituents: PBS, 1% BSA- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- Cell preparation containing CD226 protein. The exact immunogen used to generate this antibody is proprietary information. Database link Q15762
- Clone number
DX11
- Purification technique
Affinity purification Protein G
- Concentration
- Purification notes
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
Notes
ab33337 is reported to inhibit T- and NK cell mediated cytotoxicity against tumour cell targets and to block TNF alpha and IFN gamma secretion by alloantigen-specific T-cells.
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Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CD226 also known as DNAM-1 (DNAX Accessory Molecule-1) is a transmembrane protein expressed on the surface of various immune cells such as T cells natural killer (NK) cells and some B cells. The protein has a molecular weight of approximately 65 kDa. CD226 is part of the Ig superfamily and plays a role in the adhesion and signaling processes of immune cells. It interacts with its ligands CD112 (PVRL2) and CD155 (PVR) on target cells facilitating immune synapse formation and cytotoxic activity.
- Biological function summary
CD226 is involved in immune cell activation and receptor-mediated signaling. It aids the recognition and destruction of target cells by NK cells and cytotoxic T lymphocytes through its interaction with ligand-expressing cells. CD226 does not work alone but functions as part of a complex system of co-stimulatory and inhibitory receptors balancing immune responses. Its cooperation with receptors such as CD96 and TIGIT (T cell immunoreceptor with Ig and ITIM domains) modulates the activity of immune effector cells.
- Pathways
CD226 takes part in immune signaling pathways that regulate cell-mediated cytotoxicity and inflammation. It is an important component of the immune checkpoint axis interacting closely with proteins like CD96 and TIGIT within these pathways. This interaction helps determine the fate of immune responses influencing the progression or resolution of inflammatory and autoimmune conditions. CD226 plays a role in the NK cell-mediated cytotoxicity pathway and the adaptive immune response.
- Associated diseases and disorders
CD226 has been linked to autoimmune diseases such as multiple sclerosis and type 1 diabetes. Abnormal expression or function of CD226 can influence the pathogenesis of these conditions often through interactions with related proteins like TIGIT impacting immune tolerance and effector cell function. CD226's involvement in regulating immune responses highlights its importance as a potential therapeutic target in managing autoimmune and other immune-related disorders.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
![Flow Cytometry - PE Anti-CD226 antibody [DX11] (ab33337), expandable thumbnail](https://content.abcam.com/products/images/pe-cd226-antibody-dx11-ab33337--flow-cytometry-img47634.jpg "Flow Cytometry - PE Anti-CD226 antibody [DX11] (ab33337)")
Flow Cytometry - PE Anti-CD226 antibody [DX11] (ab33337)
Flow Cytometry / FACS staining of human peripheral blood lymphocytes with ab33337
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