AB77219

PE Anti-CD24 antibody [SN3]

Name: PE Anti-CD24 antibody [SN3] (ab77219) | Abcam
Brand: Abcam Limited
SKU: AB77219
Price: 110 USD
Availability: InStock
Rating: 5 (1 reviews)

(1 Review)

(11 Publications)

Mouse Monoclonal CD24 antibody - conjugated to PE. Suitable for Flow Cyt and reacts with Human samples. Cited in 11 publications.

View Alternative Names

CD24, CD24A, Signal transducer CD24, Small cell lung carcinoma cluster 4 antigen

1 Images

Flow Cytometry - PE Anti-CD24 antibody [SN3] (AB77219)

Flow Cyt

Supplier Data

Flow Cytometry - PE Anti-CD24 antibody [SN3] (AB77219)

Flow cytometry analysis (surface staining) of human peripheral blood cells with anti-human CD24 (SN3) PE.

Unconjugated

Anti-CD24 antibody [SN3]
660 APC

APC Anti-CD24 antibody [SN3]
785 APC/Cy7®

APC/Cy7® Anti-CD24 antibody [SN3]
723 Alexa Fluor® 700

Alexa Fluor® 700 Anti-CD24 antibody [SN3]
519 FITC

FITC Anti-CD24 antibody [SN3]
578 PE

PE Anti-CD24 antibody [SN3]
695 PerCP/Cy5.5®

PerCP/Cy5.5® Anti-CD24 antibody [SN3]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

SN3

Isotype

IgG1

Conjugation

Excitation/Emission

Ex: 480;565nm, Em: 578nm

Carrier free

Reacts with

Human

Applications

Flow Cyt

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "20 µL for 10^6 Cells", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/pe-mouse-igg1-b11-6-isotype-control-ab91357'>ab91357</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.Use 20μl per 100μl of whole blood or 106 cells in suspension." } } }

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Product details

Purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use.

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Properties and storage information

Form

Liquid

Purification technique

Size-exclusion chromatography

Storage buffer

pH: 7.4 Preservative: 0.097% Sodium azide Constituents: 0.2% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD24 also known as heat-stable antigen or CD24A is a glycosylphosphatidylinositol (GPI)-anchored membrane protein with a molecular mass of approximately 30-50 kDa. It is expressed on the surface of many cell types including B cells T cells neutrophils and epithelial cells. CD24 plays a role in cell adhesion and signaling processes impacting immune response and cancer progression. Its expression is often used as a marker in identification and classification procedures such as flow cytometry.

Biological function summary

CD24 functions like a molecular "switch" modulating signals sent between cells. It acts as a ligand for multiple receptors and participates in cellular communication especially influencing the immune system. Part of its biological role involves interaction within cellular complexes impacting immune modulation and inflammatory responses. CD24's location at the interface of cellular microenvironments makes it critical for cellular response adaptation to varied external stimuli.

Pathways

CD24 participates in both immune signaling and cancer-related pathways. It plays a significant role in the suppression of the immune response often interacting with proteins like Siglec-10 in these pathways. CD24's involvement in these pathways contributes to cell survival and proliferation particularly within cancer development. The protein’s interaction within specified signaling pathways allows it to influence downstream targets that regulate gene expression and cellular functions.

CD24 has significant connections to cancer and inflammatory diseases. It is highly expressed in various cancers including breast and prostate cancer where it correlates with poor prognosis due to its role in apoptosis evasion and metastasis. CD24 interacts with proteins like HMGB1 and is implicated in inflammatory conditions where it modulates immune cell activity. Extensive research into CD24's involvement in these disorders may lead to targeted therapies including the development of anti-CD24 antibodies for therapeutic interventions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

May have a pivotal role in cell differentiation of different cell types. Signaling could be triggered by the binding of a lectin-like ligand to the CD24 carbohydrates, and transduced by the release of second messengers derived from the GPI-anchor. Modulates B-cell activation responses. Promotes AG-dependent proliferation of B-cells, and prevents their terminal differentiation into antibody-forming cells (PubMed : 11313396). In association with SIGLEC10 may be involved in the selective suppression of the immune response to danger-associated molecular patterns (DAMPs) such as HMGB1, HSP70 and HSP90. Plays a role in the control of autoimmunity (By similarity).

See full target information CD24

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Publications (11)

Recent publications for all applications. Explore the full list and refine your search

Cellular signalling 84:110025 PubMed33915247

2021

microRNA-873 inhibits self-renewal and proliferation of pancreatic cancer stem cells through pleckstrin-2-dependent PI3K/AKT pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao-Li Yang,Yu-Shui Ma,Yu-Shan Liu,Xiao-Hui Jiang,Hua Ding,Yi Shi,Cheng-You Jia,Gai-Xia Lu,Dan-Dan Zhang,Hui-Min Wang,Pei-Yao Wang,Zhong-Wei Lv,Fei Yu,Ji-Bin Liu,Da Fu

International journal of molecular sciences 21: PubMed33260837

2020

EGFR Expression in HER2-Driven Breast Cancer Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Florian Weinberg,Diana B Peckys,Niels de Jonge

Clinical & experimental metastasis 35:831-846 PubMed30377878

2018

Chemotherapy resistance and stemness in mitotically quiescent human breast cancer cells identified by fluorescent dye retention.

Applications

Unspecified application

Species

Unspecified reactive species

Lewis A Quayle,Penelope D Ottewell,Ingunn Holen

Molecular biology of the cell : PubMed28794264

2017

Liquid-phase electron microscopy of molecular drug response in breast cancer cells reveals irresponsive cell subpopulations related to lack of HER2 homodimers.

Applications

Unspecified application

Species

Unspecified reactive species

Diana B Peckys,Ulrike Korf,Stefan Wiemann,Niels de Jonge

PloS one 11:e0164250 PubMed27723829

2016

Intrinsic Resistance to 5-Fluorouracil in a Brain Metastatic Variant of Human Breast Cancer Cell Line, MDA-MB-231BR.

Applications

Unspecified application

Species

Unspecified reactive species

Atsunobu Sagara,Katsuhide Igarashi,Maky Otsuka,Takeshi Karasawa,Noriko Gotoh,Michiko Narita,Naoko Kuzumaki,Minoru Narita,Yoshinori Kato

Stem cells and development 22:444-58 PubMed23050522

2012

Human mid-trimester amniotic fluid stem cells cultured under embryonic stem cell conditions with valproic acid acquire pluripotent characteristics.

Applications

Flow Cyt

Species

Human

Dafni Moschidou,Sayandip Mukherjee,Michael P Blundell,Gemma N Jones,Anthony J Atala,Adrian J Thrasher,Nicholas M Fisk,Paolo De Coppi,Pascale V Guillot

Gastroenterology 143:1375-1384.e5 PubMed22819865

2012

Redirected T cells that target pancreatic adenocarcinoma antigens eliminate tumors and metastases in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Amit Maliar,Charlotte Servais,Tova Waks,Markus Chmielewski,Ron Lavy,Peter Altevogt,Hinrich Abken,Zelig Eshhar

The Journal of experimental medicine 175:397-404 PubMed1370683

1992

The human fetal omentum: a site of B cell generation.

Applications

Unspecified application

Species

Unspecified reactive species

N Solvason,J F Kearney

Journal of immunology (Baltimore, Md. : 1950) 144:638-41 PubMed2153173

1990

Signal transduction in lymphocytic and myeloid cells via CD24, a new member of phosphoinositol-anchored membrane molecules.

Applications

Unspecified application

Species

Unspecified reactive species

G F Fischer,O Majdic,S Gadd,W Knapp

Hematological oncology 4:251-9 PubMed3493967

1986

Follicular mantle zone cell subpopulations detected by monoclonal antibody SN3.

Applications

Unspecified application

Species

Unspecified reactive species

M Barcos,C Pollard,T Fukukawa,B K Seon

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

PE Anti-CD24 antibody [SN3]

Flow Cytometry - PE Anti-CD24 antibody [SN3] (AB77219)

Related conjugates and formulations (7)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (11)

Product promise