AB176502

PE Anti-CD28 antibody [JJ319]

Name: Anti-CD28 PE antibody [JJ319] (ab176502) Mouse monoclonal IgG1 | Abcam
Brand: Abcam Limited
SKU: AB176502
Price: 195 USD
Availability: InStock

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(2 Publications)

Mouse Monoclonal CD28 antibody - conjugated to PE. Suitable for Flow Cyt and reacts with Rat samples. Cited in 2 publications. Immunogen corresponding to Cell preparation containing Cd28 protein.

View Alternative Names

CD28, T-cell-specific surface glycoprotein CD28, Cd28

2 Images

Flow Cytometry - PE Anti-CD28 antibody [JJ319] (AB176502)

Flow Cyt

Unknown

Flow Cytometry - PE Anti-CD28 antibody [JJ319] (AB176502)

Flow cytometry surface staining pattern of rat splenocytes stained using ab176502

Flow Cyt

Unknown

Flow Cytometry - PE Anti-CD28 antibody [JJ319] (AB176502)

Separation of rat CD28 positive splenocytes (red-filled) from CD28 negative splenocytes (black-dashed) in flow cytometry analysis (surface staining) of murine splenocyte suspension stained using ab176502

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

JJ319

Isotype

IgG1

Conjugation

Excitation/Emission

Ex: 480;565nm, Em: 578nm

Carrier free

Reacts with

Rat

Applications

Flow Cyt

applications

Immunogen

Cell preparation containing Cd28 protein. The exact immunogen used to generate this antibody is proprietary information.

P31041

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Rat": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1-5 µg/mL", "FlowCyt-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Size-exclusion chromatography

Storage buffer

pH: 7.4 Preservative: 0.098% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Store in the dark

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD28 also known as T-cell-specific surface glycoprotein CD28 or TP44 plays an important role as a co-stimulatory receptor on T cells. This glycoprotein has a molecular mass of about 44 kDa and is located mainly on the cell surface of T cells. CD28 expression also occurs on thymocytes and some B cells. It forms part of the immunoglobulin superfamily featuring in significant antibody interactions. Researchers often use anti-CD28 antibodies for various immunological assays including CD28 ELISA to study its function.

Biological function summary

CD28 co-stimulates T cells after antigen presentation. It enhances T cell proliferation survival and cytokine production. CD28 functions alongside the T cell receptor (TCR) complex mediating signals that promote full T cell activation. This interaction amplifies the immune response forming an important axis of immune system communication. CD28 interacts with its ligands B7-1 (CD80) and B7-2 (CD86) found on antigen-presenting cells (APCs) illustrating its role in immune system dynamics.

Pathways

CD28 is integral to the PI3K/Akt and MAPK/ERK signaling pathways. These pathways conduct signals critical for cellular growth and survival linking CD28 to downstream transcription factors. The interaction with another protein CTLA-4 acts as a regulatory balance for CD28 signaling preventing over-activation of T cells. This balance influences immune homeostasis and tolerance providing a framework for understanding costimulatory and inhibitory signaling.

CD28's role becomes evident in autoimmune diseases and transplant rejection. Abnormal CD28 signaling links to rheumatoid arthritis where inappropriate T cell activation occurs. Similarly CD28 activity contributes to organ transplant rejection by stimulating T cell-mediated responses against transplanted tissues. CTLA-4 through regulatory feedback can modify these effects providing therapeutic target strategies for managing such disorders. Efforts to modulate CD28 activity involve developing therapeutic agents such as anti-CD28 monoclonal antibodies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Receptor that plays a role in T-cell activation, proliferation, survival and the maintenance of immune homeostasis (PubMed : 7688139). Functions not only as an amplifier of TCR signals but delivers unique signals that control intracellular biochemical events that alter the gene expression program of T-cells. Stimulation upon engagement of its cognate ligands CD80 or CD86 increases proliferation and expression of various cytokines in particular IL2 production in both CD4(+) and CD8(+) T-cell subsets. Mechanistically, ligation induces recruitment of protein kinase C-theta/PRKCQ and GRB2 leading to NF-kappa-B activation via both PI3K/Akt-dependent and -independent pathways. In conjunction with TCR/CD3 ligation and CD40L costimulation, enhances the production of IL4 and IL10 in T-cells.

See full target information Cd28

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Nutrients 16: PubMed39683500

2024

Omega-3 Fatty Acids Weaken Lymphocyte Inflammatory Features and Improve Glycemic Control in Nonobese Diabetic Goto-Kakizaki Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Tiago Bertola Lobato,Elvirah Samantha de Sousa Santos,Patrícia Nancy Iser-Bem,Henrique de Souza Falcão,Gabriela Mandú Gimenes,Janaina Ribeiro Barbosa Pauferro,Glayce Tavares Rodrigues,Ilana Souza Correa,Ana Carolina Gomes Pereira,Maria Elizabeth Pereira Passos,João Carlos de Oliveira Borges,Amara Cassandra Dos Anjos Alves,Camila Soares Dos Santos,Maria Janaina Leite de Araújo,Vinícius Leonardo Sousa Diniz,Adriana Cristina Levada-Pires,Tânia Cristina Pithon-Curi,Laureane Nunes Masi,Rui Curi,Sandro Massao Hirabara,Renata Gorjão

Science advances 7: PubMed33637530

2021

Tumor-specific cytolytic CD4 T cells mediate immunity against human cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Amélie Cachot,Mariia Bilous,Yen-Cheng Liu,Xiaokang Li,Margaux Saillard,Mara Cenerenti,Georg Alexander Rockinger,Tania Wyss,Philippe Guillaume,Julien Schmidt,Raphaël Genolet,Giuseppe Ercolano,Maria Pia Protti,Walter Reith,Kalliopi Ioannidou,Laurence de Leval,Joseph A Trapani,George Coukos,Alexandre Harari,Daniel E Speiser,Alexander Mathis,David Gfeller,Hatice Altug,Pedro Romero,Camilla Jandus

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

PE Anti-CD28 antibody [JJ319]

Flow Cytometry - PE Anti-CD28 antibody [JJ319] (AB176502)

Flow Cytometry - PE Anti-CD28 antibody [JJ319] (AB176502)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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