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AB22268

PE Anti-CD3 antibody [KT3]

2

(8 Reviews)

|

(8 Publications)

Rat Monoclonal CD3d antibody - conjugated to PE. Suitable for Flow Cyt and reacts with Mouse samples. Cited in 8 publications.

View Alternative Names

CD3d, T3D, CD3D, T-cell surface glycoprotein CD3 delta chain, T-cell receptor T3 delta chain

3 Images
Flow Cytometry - PE Anti-CD3 antibody [KT3] (AB22268)
  • Flow Cyt

AbReview26831****

Flow Cytometry - PE Anti-CD3 antibody [KT3] (AB22268)

Flow cytometry analysis of human peripheral blood mononuclear cells (PBMC) labelling CD3 with ab22268 at a dilution of (1/100 in PBS + 0.5% BSA) for 1 hour at 4°C. Cell separation was achieved by FICOLL medium with PBS + 0.5% BSA.
Gating Strategy : Lymphocytes

This image is courtesy of an anonymous Abreview

Flow Cytometry - PE Anti-CD3 antibody [KT3] (AB22268)
  • Flow Cyt

AbReview25711****

Flow Cytometry - PE Anti-CD3 antibody [KT3] (AB22268)

ab22268 used in Flow Cytometry.

Canine blood leucocytes were harvested in PBS, 2% BSA, 0.01% sodium azide after red blood cell lysis, then fixed with paraformaldehyde. ab22268 used at a 1/100 dilution for 1 hour at 4°C.

The gating strategy used was for lymphocytes.

Image courtesy of an anonymous Abreview.

Flow Cytometry - PE Anti-CD3 antibody [KT3] (AB22268)
  • Flow Cyt

Unknown

Flow Cytometry - PE Anti-CD3 antibody [KT3] (AB22268)

ab22268 staining CD3 in mouse spleen cells by Flow Cytometry analysis.

Key facts

Host species

Rat

Clonality

Monoclonal

Clone number

KT3

Isotype

IgG2a

Conjugation

PE

Excitation/Emission

Ex: 480;565nm, Em: 578nm

Carrier free

No

Reacts with

Mouse

Applications

Flow Cyt

applications

Specificity

This antibody recognises the mouse CD3 antigen which is expressed by mature T cells.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "10 µL for 10^6 Cells", "FlowCyt-species-notes": "<p><a href='/en-us/products/unavailable/rat-igg2a-kappa-monoclonal-klhg2a-1-1-pe-isotype-control-ab134674'>ab134674</a> - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.</p>" } } }
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Product details

Purified IgG conjugated to R. Phycoerythrin (RPE).
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Preservative: 0.09% Sodium azide Constituents: PBS, 5% Sucrose, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD247/CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways (PubMed : 2470098). In addition of this role of signal transduction in T-cell activation, CD3D plays an essential role in thymocyte differentiation. Indeed, participates in correct intracellular TCR-CD3 complex assembly and surface expression. In absence of a functional TCR-CD3 complex, thymocytes are unable to differentiate properly. Interacts with CD4 and CD8 and thus serves to establish a functional link between the TCR and coreceptors CD4 and CD8, which is needed for activation and positive selection of CD4 or CD8 T-cells (PubMed : 12215456).
See full target information CD3D
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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Journal of nanobiotechnology 23:469 PubMed40598231

2025

Targeting YTHDF2 with pH-responsive siRNA nanoparticles suppresses MYC m6A modification and restores antitumor immunity in hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Ziqi Guo,Qiuling Huang,Zhenzhen Cui,Cheng Yang,Liu Yang

Cell biology and toxicology 41:82 PubMed40335763

2025

ELF4 improves sepsis-induced myocardial injury by regulating STING signaling-mediated T cells differentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Yawen Zheng,Xiongjun Peng,Yusha Zhang,Ruilin Liu,Junke Long

Communications biology 8:514 PubMed40155473

2025

Defining gastric cancer ecology: the crucial roles of TREM2 macrophages and fibroblasts in tumor microenvironments.

Applications

Unspecified application

Species

Unspecified reactive species

Qianqian Cao,Dianshui Sun,Can Tu,Jihua Wang,Runjia Fu,Rumei Gong,Yueying Xiao,Qin Liu,Xiaomei Li

Immunotherapy 16:975-985 PubMed39115961

2024

The development of anti-PD-1 antibody-induced spinal cord injury in bone marrow transplant C57BL/6 mouse model.

Applications

Unspecified application

Species

Unspecified reactive species

Huachun Chen,Zhouxiao Lu,Xiaowei Ni,Hui Zhang,Guiyuan Chen,Xiaoyu Wu,Mingxing Ding

International immunopharmacology 138:112546 PubMed38917522

2024

MC1R regulates T regulatory cell differentiation through metabolic reprogramming to promote colon cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Shaoliang Zhu,Mengjie Zou,Chunxing Li,Yuntian Tang,Honglin Luo,Xiaofeng Dong

Cell death & disease 15:69 PubMed38238314

2024

The P286R mutation of DNA polymerase ε activates cancer-cell-intrinsic immunity and suppresses endometrial tumorigenesis via the cGAS-STING pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Ming Tang,Shasha Yin,Hongliang Zeng,Ao Huang,Yujia Huang,Zhiyi Hu,Ab Rauf Shah,Shuyong Zhang,Haisen Li,Guofang Chen

Nature nanotechnology 17:531-540 PubMed35410368

2022

A nanovaccine for antigen self-presentation and immunosuppression reversal as a personalized cancer immunotherapy strategy.

Applications

Unspecified application

Species

Unspecified reactive species

Chao Liu,Xue Liu,Xinchu Xiang,Xin Pang,Siyuan Chen,Yunming Zhang,En Ren,Lili Zhang,Xuan Liu,Peng Lv,Xiaoyong Wang,Wenxin Luo,Ningshao Xia,Xiaoyuan Chen,Gang Liu

BMC veterinary research 14:119 PubMed29606140

2018

Identification of immunologic and clinical characteristics that predict inflammatory response to C. Novyi-NT bacteriolytic immunotherapy.

Applications

Unspecified application

Species

Dog

Amy E DeClue,Sandra M Axiak-Bechtel,Yan Zhang,Saurabh Saha,Linping Zhang,David D Tung,Jeffrey N Bryan
View all publications
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Product promise

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