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Rat Monoclonal CD3d antibody - conjugated to PE. Suitable for Flow Cyt and reacts with Mouse samples. Cited in 2 publications.


Images

Flow Cytometry - PE Anti-CD3 antibody [KT3] (AB22268), expandable thumbnail
  • Flow Cytometry - PE Anti-CD3 antibody [KT3] (AB22268), expandable thumbnail
  • Flow Cytometry - PE Anti-CD3 antibody [KT3] (AB22268), expandable thumbnail

Publications

Key facts

Isotype
IgG2a
Host species
Rat
Conjugation
PE
Excitation/Emission
Ex: 480;565nm, Em: 578nm
Storage buffer

Preservative: 0.09% Sodium azide
Constituents: PBS, 5% Sucrose, 1% BSA

Form
Liquid
Clonality
Monoclonal

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow Cyt
Mouse
Tested

Tested
Tested

Species
Mouse
Dilution info
10 µL for 106 Cells
Notes

ab134674 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

Associated Products

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7 products for Alternative Product

Target data

Function

Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways (PubMed:2470098). In addition of this role of signal transduction in T-cell activation, CD3D plays an essential role in thymocyte differentiation. Indeed, participates in correct intracellular TCR-CD3 complex assembly and surface expression. In absence of a functional TCR-CD3 complex, thymocytes are unable to differentiate properly. Interacts with CD4 and CD8 and thus serves to establish a functional link between the TCR and coreceptors CD4 and CD8, which is needed for activation and positive selection of CD4 or CD8 T-cells (PubMed:12215456).

Alternative names

Recommended products

Rat Monoclonal CD3d antibody - conjugated to PE. Suitable for Flow Cyt and reacts with Mouse samples. Cited in 2 publications.

Key facts

Isotype
IgG2a
Conjugation
PE
Excitation/Emission
Ex: 480;565nm, Em: 578nm
Form
Liquid
Clonality
Monoclonal
Clone number
KT3
Purification technique
Affinity purification Protein A
Specificity

This antibody recognises the mouse CD3 antigen which is expressed by mature T cells.

Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C

Notes

Purified IgG conjugated to R. Phycoerythrin (RPE).

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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3 product images

  • Flow Cytometry - PE Anti-CD3 antibody [KT3] (ab22268), expandable thumbnail
    Image courtesy of an anonymous customer review.

    Flow Cytometry - PE Anti-CD3 antibody [KT3] (ab22268)

    ab22268 used in Flow Cytometry.

    Canine blood leucocytes were harvested in PBS, 2% BSA, 0.01% sodium azide after red blood cell lysis, then fixed with paraformaldehyde. ab22268 used at a 1/100 dilution for 1 hour at 4°C.

    The gating strategy used was for lymphocytes.

  • Flow Cytometry - PE Anti-CD3 antibody [KT3] (ab22268), expandable thumbnail

    Flow Cytometry - PE Anti-CD3 antibody [KT3] (ab22268)

    ab22268 staining CD3 in mouse spleen cells by Flow Cytometry analysis.

  • Flow Cytometry - PE Anti-CD3 antibody [KT3] (ab22268), expandable thumbnail
    This image is courtesy of an anonymous customer review

    Flow Cytometry - PE Anti-CD3 antibody [KT3] (ab22268)

    Flow cytometry analysis of human peripheral blood mononuclear cells (PBMC) labelling CD3 with ab22268 at a dilution of (1/100 in PBS + 0.5% BSA) for 1 hour at 4°C. Cell separation was achieved by FICOLL medium with PBS + 0.5% BSA.
    Gating Strategy: Lymphocytes

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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