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Rabbit Recombinant Monoclonal CD300a antibody - conjugated to PE. Suitable for Flow Cyt and reacts with Human samples.

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Images

Flow Cytometry - PE Anti-CD300a antibody [EPR27995-84] (AB323732), expandable thumbnail
  • Flow Cytometry - PE Anti-CD300a antibody [EPR27995-84] (AB323732), expandable thumbnail
  • Flow Cytometry - PE Anti-CD300a antibody [EPR27995-84] (AB323732), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Conjugation
PE
Excitation/Emission
Ex: 480;565nm, Em: 578nm
Storage buffer

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98% PBS, 1% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow Cyt
Human
Tested

Tested
Tested

Species
Human
Dilution info
1/500
Notes

-

Associated Products

Select an associated product type

1 product for Isotype control

Target data

Function

Inhibitory receptor which may contribute to the down-regulation of cytolytic activity in natural killer (NK) cells, and to the down-regulation of mast cell degranulation (PubMed:10746781, PubMed:16339535, PubMed:9701027). Negatively regulates the Toll-like receptor (TLR) signaling mediated by MYD88 but not TRIF through activation of PTPN6 (PubMed:22043923).

Alternative names

CD300a, CMRF35H, IGSF12, HSPC083, CD300A, CMRF35-like molecule 8, CLM-8, CD300 antigen-like family member A, CMRF-35-H9, CMRF35-H, IRC1/IRC2, Immunoglobulin superfamily member 12, Inhibitory receptor protein 60, NK inhibitory receptor, CMRF35-H9, IgSF12, IRp60

Rabbit Recombinant Monoclonal CD300a antibody - conjugated to PE. Suitable for Flow Cyt and reacts with Human samples.

Key facts

Isotype
IgG
Conjugation
PE
Excitation/Emission
Ex: 480;565nm, Em: 578nm
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR27995-84
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle, Store in the dark

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Flow Cytometry - PE Anti-CD300a antibody [EPR27995-84] (ab323732), expandable thumbnail

    Flow Cytometry - PE Anti-CD300a antibody [EPR27995-84] (ab323732)

    CD300a Flow Cytometry staining of Human PBMC (human peripheral blood mononuclear cell) using rabbit Anti-CD300a antibody

    Flow cytometric analysis of Human PBMC (human peripheral blood mononuclear cell) cells labelling CD300a with ab323732 at 1/500 dilution (0.1ug) / Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Phycoerythrin) (PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab209478) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

    Cells were co-stained with anti human CD19 conjugated to Alexa Fluor® 647.

    Gated on viable cells.

  • Flow Cytometry - PE Anti-CD300a antibody [EPR27995-84] (ab323732), expandable thumbnail

    Flow Cytometry - PE Anti-CD300a antibody [EPR27995-84] (ab323732)

    CD300a Flow Cytometry staining of Human PBMC (human peripheral blood mononuclear cell) using rabbit Anti-CD300a antibody

    Flow cytometric analysis of Human PBMC (human peripheral blood mononuclear cell) cells labelling CD300a with ab323732 at 1/500 dilution (0.1ug) / Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Phycoerythrin) (PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab209478) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

    Cells were co-stained with anti human CD14 conjugated to Brilliant Violet 421.

    Gated on viable cells.

  • Flow Cytometry - PE Anti-CD300a antibody [EPR27995-84] (ab323732), expandable thumbnail

    Flow Cytometry - PE Anti-CD300a antibody [EPR27995-84] (ab323732)

    CD300a Flow Cytometry staining using rabbit Anti-CD300a antibody

    Flow cytometric analysis of 293T (human embryonic kidney epithelial cell, Left) / THP-1 (human monocytic leukemia monocyte, Right) cells labelling CD300a with ab323732 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Phycoerythrin) (PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab209478) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

    Gated on viable cells.

    Negative control: 293T (PMID: 22043923)

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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