Mouse Monoclonal CD31 antibody - conjugated to PE. Suitable for Flow Cyt and reacts with Human samples. Immunogen corresponding to Cell preparation containing PECAM1 protein.
Images
![Flow Cytometry - PE Anti-CD31 antibody [MEM-05] (AB269307), expandable thumbnail](https://content.abcam.com/products/images/pe-cd31-antibody-mem-05-ab269307--flow-cytometry-img94728.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - PE Anti-CD31 antibody [MEM-05] (AB269307)")
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Conjugation
- PE
- Excitation/Emission
- Ex: 480;565nm, Em: 578nm
- Storage buffer
pH: 7.4
Preservative: 0.1% Sodium azide
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Cell preparation containing PECAM1 protein. The exact immunogen used to generate this antibody is proprietary information. Database link P16284
Reactivity data
Select an application| Flow Cyt | |
|---|---|
| Human | Tested |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 20 µL for 106 Cells | Notes Or 100 μL of whole blood. |
Associated Products
Select an associated product type
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7 products for Alternative Product
Target data
Function
Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions (PubMed:17580308, PubMed:19342684). Tyr-690 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes (PubMed:19342684). Trans-homophilic interaction may play a role in endothelial cell-cell adhesion via cell junctions (PubMed:27958302). Heterophilic interaction with CD177 plays a role in transendothelial migration of neutrophils (PubMed:17580308). Homophilic ligation of PECAM1 prevents macrophage-mediated phagocytosis of neighboring viable leukocytes by transmitting a detachment signal (PubMed:12110892). Promotes macrophage-mediated phagocytosis of apoptotic leukocytes by tethering them to the phagocytic cells; PECAM1-mediated detachment signal appears to be disabled in apoptotic leukocytes (PubMed:12110892). Modulates bradykinin receptor BDKRB2 activation (PubMed:18672896). Regulates bradykinin- and hyperosmotic shock-induced ERK1/2 activation in endothelial cells (PubMed:18672896). Induces susceptibility to atherosclerosis (By similarity). Isoform Delta15. Does not protect against apoptosis.
Alternative names
CD31, Platelet endothelial cell adhesion molecule, PECAM-1, EndoCAM, GPIIA', PECA1, PECAM1
Recommended products
Mouse Monoclonal CD31 antibody - conjugated to PE. Suitable for Flow Cyt and reacts with Human samples. Immunogen corresponding to Cell preparation containing PECAM1 protein.
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Conjugation
- PE
- Excitation/Emission
- Ex: 480;565nm, Em: 578nm
- Storage buffer
pH: 7.4
Preservative: 0.1% Sodium azide
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Cell preparation containing PECAM1 protein. The exact immunogen used to generate this antibody is proprietary information. Database link P16284
- Clone number
- MEM-05
- Purification technique
- Size-exclusion chromatography
- Concentration
- Purification notes
The purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Store in the dark
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
![Flow Cytometry - PE Anti-CD31 antibody [MEM-05] (ab269307), expandable thumbnail](https://content.abcam.com/products/images/pe-cd31-antibody-mem-05-ab269307--flow-cytometry-img94728.jpg "Flow Cytometry - PE Anti-CD31 antibody [MEM-05] (ab269307)")
Flow Cytometry - PE Anti-CD31 antibody [MEM-05] (ab269307)
Flow cytometry analysis (surface staining) of human peripheral blood labeling CD31 with ab269307.
Downloads
Product protocols
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