Rabbit Recombinant Monoclonal CD34 antibody - conjugated to PE. Suitable for Flow Cyt (Intra) and reacts with Human samples. Cited in 7 publications.
Images
![Flow Cytometry (Intracellular) - PE Anti-CD34 antibody [EP373Y] (AB223930), expandable thumbnail](https://content.abcam.com/products/images/pe-cd34-antibody-ep373y-ab223930--flow-cytometry-intracellular-img25971.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - PE Anti-CD34 antibody [EP373Y] (AB223930)")
Publications
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- Scientific reports 13:150412023Combined effect of DBM, PRP, and bone marrow fluid on bone union in a rat posterolateral fusion model.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTakuma Otagiri,Yasuhiro Shiga,Takashi Hozumi,Yusuke Matsuura,Ikuko Tajiri,Naoya Takayama,Maria Alejandra,Yuki Shiko,Sumihisa Orita,Kazuhide Inage,Yawara Eguchi,Takane Suzuki,Miyako Suzuki-Narita,Michiaki Mukai,Tomohito Mukaihata,Ryuto Tsuchiya,Soichiro Tokeshi,Kohei Okuyama,Takahito Arai,Noriyasu Toshi,Takeo Furuya,Satoshi Maki,Yasuchika Aoki,Seiji OhtoriPubMed 37699916
- European journal of histochemistry : EJH 66:2022Extracellular vesicles from bone mesenchymal stem cells transport microRNA-206 into osteosarcoma cells and target NRSN2 to block the ERK1/2-Bcl-xL signaling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAlimu Keremu,Pazila Aila,Aikebaier Tusun,Maimaitiaili Abulikemu,Xiaoguang ZouPubMed 35730574
- Experimental and therapeutic medicine 22:14152021Adipose mesenchymal stem cells and gingival mesenchymal stem cells have a comparable effect in endothelium repair.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKe Yang,Dongmei Xie,Wanwen Lin,Peng Xiang,Chaoquan PengPubMed 34676008
- Journal of nanobiotechnology 19:1942021Mesenchymal stem cell-derived extracellular vesicles prevent the development of osteoarthritis via the circHIPK3/miR-124-3p/MYH9 axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShenglong Li,Jie Liu,Siyu Liu,Weijie Jiao,Xiaohong WangPubMed 34193158
- Cell cycle (Georgetown, Tex.) 19:3608-36212020MiR-17-5p promotes the endothelialization of endothelial progenitor cells to facilitate the vascular repair of aneurysm by regulating PTEN-mediated PI3K/AKT/VEGFA pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYe Tian,Xinxi Li,Chao Bai,Zhenwei Yang,Lei Zhang,Jun LuoPubMed 33315517
- Molecular medicine reports 20:664-6702019Substance P enhances BMSC osteogenic differentiation via autophagic activation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWen Geng et. al.PubMed 31115537
- Scientific reports 7:441332017A potential regulatory network underlying distinct fate commitment of myogenic and adipogenic cells in skeletal muscle.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWenjuan Sun et. al.PubMed 28276486
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Conjugation
- PE
- Excitation/Emission
- Ex: 480;565nm, Em: 578nm
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| Flow Cyt (Intra) | |
|---|---|
| Human | Tested |
| Mouse | Predicted |
| Rat | Predicted |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500 | Notes The cellular localisation of this product has been verified in ICC/IF. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
15 products for Alternative Product
3 products for Alternative Version
Target data
Function
Possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins.
Alternative names
CD34, Hematopoietic progenitor cell antigen CD34
Recommended products
Rabbit Recombinant Monoclonal CD34 antibody - conjugated to PE. Suitable for Flow Cyt (Intra) and reacts with Human samples. Cited in 7 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Conjugation
- PE
- Excitation/Emission
- Ex: 480;565nm, Em: 578nm
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EP373Y
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Do Not Freeze, Store in the dark
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
![Flow Cytometry (Intracellular) - PE Anti-CD34 antibody [EP373Y] (ab223930), expandable thumbnail](https://content.abcam.com/products/images/pe-cd34-antibody-ep373y-ab223930--flow-cytometry-intracellular-img25971.jpg "Flow Cytometry (Intracellular) - PE Anti-CD34 antibody [EP373Y] (ab223930)")
Flow Cytometry (Intracellular) - PE Anti-CD34 antibody [EP373Y] (ab223930)
Overlay histogram showing HUVEC cells stained with ab223930 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab223930, 1/500 dilution) for 30 min at 22°C.
Isotype control antibody (black line) was Rabbit IgG (monoclonal) Phycoerythrin (PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5,000 events were collected using a 50 mW Yellow/Green laser (561nm) and 586/15 bandpass filter.
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