Mouse Monoclonal CD35 antibody - conjugated to PE. Suitable for Flow Cyt and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Cell preparation containing CR1 protein.
Images
![Flow Cytometry - PE Anti-CD35 antibody [E11] (AB178451), expandable thumbnail](https://content.abcam.com/products/images/pe-cd35-antibody-e11-ab178451--flow-cytometry-img438216.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - PE Anti-CD35 antibody [E11] (AB178451)")
![Flow Cytometry - PE Anti-CD35 antibody [E11] (AB178451), expandable thumbnail](https://content.abcam.com/products/images/pe-cd35-antibody-e11-ab178451--flow-cytometry-img438217.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - PE Anti-CD35 antibody [E11] (AB178451)")
Publications
Filter by
- Cytometry. Part A : the journal of the International Society for Analytical Cytology 81:165-82011Identification of a cross-reacting, monoclonal anti-human CD233 antibody for identification and sorting of rhesus macaque erythrocytes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesColleen Byrnes,Y Terry Lee,Robert E Donahue,Jeffery L MillerPubMed 22170815
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Conjugation
- PE
- Excitation/Emission
- Ex: 480;565nm, Em: 578nm
- Storage buffer
pH: 7.4
Preservative: 0.1% Sodium azide
Constituents: 99% PBS, 0.2% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Cell preparation containing CR1 protein. The exact immunogen used to generate this antibody is proprietary information. Database link P17927
Reactivity data
Select an application| Flow Cyt | |
|---|---|
| Human | Tested |
| Primates | Predicted |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Primates | Dilution info - | Notes - |
Associated Products
Select an associated product type
7 products for Alternative Product
1 product for Alternative Version
Target data
Function
Membrane immune adherence receptor that plays a critical role in the capture and clearance of complement-opsonized pathogens by erythrocytes and monocytes/macrophages (PubMed:2963069). Mediates the binding by these cells of particles and immune complexes that have activated complement to eliminate them from the circulation (PubMed:2963069). Acts also in the inhibition of spontaneous complement activation by impairing the formation and function of the alternative and classical pathway C3/C5 convertases, and by serving as a cofactor for the cleavage by factor I of C3b to iC3b, C3c and C3d,g, and of C4b to C4c and C4d (PubMed:2972794, PubMed:8175757). Also plays a role in immune regulation by contributing, upon ligand binding, to the generation of regulatory T cells from activated helper T cells (PubMed:25742728). (Microbial infection) Acts as a receptor for Epstein-Barr virus.
Alternative names
CD35, C3BR, CR1, Complement receptor type 1, C3b/C4b receptor
Recommended products
Mouse Monoclonal CD35 antibody - conjugated to PE. Suitable for Flow Cyt and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Cell preparation containing CR1 protein.
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Conjugation
- PE
- Excitation/Emission
- Ex: 480;565nm, Em: 578nm
- Storage buffer
pH: 7.4
Preservative: 0.1% Sodium azide
Constituents: 99% PBS, 0.2% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Cell preparation containing CR1 protein. The exact immunogen used to generate this antibody is proprietary information. Database link P17927
- Clone number
- E11
- Purification technique
- Size-exclusion chromatography
- Specificity
This antibody recognizes CD35, a type I glycoprotein expressed on granulocytes, monocytes, B cells, folicular dendritic cells, erythrocytes, NK and T cell subsets, as well as e.g. on glomerulal podocytes.
- Concentration
- Purification notes
The purified antibody is conjugated with R-Phycoerythrin (PE) under optimumconditions. The conjugate is purified by size-exclusion chromatography andadjusted for direct use.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze, Store in the dark
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Flow Cytometry - PE Anti-CD35 antibody [E11] (ab178451), expandable thumbnail](https://content.abcam.com/products/images/pe-cd35-antibody-e11-ab178451--flow-cytometry-img438216.jpg "Flow Cytometry - PE Anti-CD35 antibody [E11] (ab178451)")
Flow Cytometry - PE Anti-CD35 antibody [E11] (ab178451)
Flow cytometric analysis of Human peripheral whole blood cells labelling CD35 with ab178451 at 0.56 μg/ml.
![Flow Cytometry - PE Anti-CD35 antibody [E11] (ab178451), expandable thumbnail](https://content.abcam.com/products/images/pe-cd35-antibody-e11-ab178451--flow-cytometry-img438217.jpg "Flow Cytometry - PE Anti-CD35 antibody [E11] (ab178451)")
Flow Cytometry - PE Anti-CD35 antibody [E11] (ab178451)
Flow cytometric analysis of Human peripheral whole blood cells labelling CD35 with ab178451 at 0.56 μg/ml.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com