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AB305817

PE Anti-CD3G antibody [EPR4517]

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Rabbit Recombinant Monoclonal CD3G antibody - conjugated to PE. Suitable for Flow Cyt (Intra), ICC/IF, IHC-P and reacts with Human, Mouse samples.

View Alternative Names

CD3g, T3G, CD3G, T-cell surface glycoprotein CD3 gamma chain, T-cell receptor T3 gamma chain

3 Images
Flow Cytometry (Intracellular) - PE Anti-CD3G antibody [EPR4517] (AB305817)
  • Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - PE Anti-CD3G antibody [EPR4517] (AB305817)

Flow cytometry staining of human peripheral blood mononuclear cells (PBMCs) with ab305817 (right) or Rabbit IgG (monoclonal) Phycoerythrin Isotype Control (ab209478) (left). Cells were fixed and permeabilised with BD Cytofix/Cytoperm™ for 20 min. PBMCs were incubated for 30 min at 4°C in 1x PBS containing 10 μg/ml human IgG and 10% normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody ab305817 or Rabbit IgG (monoclonal) Phycoerythrin Isotype Control (ab209478) (1x 106 in 100 μl at 0.04 μg/ml (1/12500)) for 30 min at 4°C . The cells were simultaneously stained with CD19.

Acquisition of >30000 events were collected using a 50mW Yellow/Green laser (561nm) and 585/42 bandpass filter. Events were gated on viable cells.

Flow Cytometry (Intracellular) - PE Anti-CD3G antibody [EPR4517] (AB305817)
  • Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - PE Anti-CD3G antibody [EPR4517] (AB305817)

Flow cytometry staining of human peripheral blood mononuclear cells (PBMCs) with ab305817 (right) or Rabbit IgG (monoclonal) Phycoerythrin Isotype Control (ab209478) (left). Cells were fixed and permeabilised with BD Cytofix/Cytoperm™ for 20 min. PBMCs were incubated for 30 min at 4°C in 1x PBS containing 10 μg/ml human IgG and 10% normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody ab305817 or Rabbit IgG (monoclonal) Phycoerythrin Isotype Control (ab209478) (1x 106 in 100 μl at 0.04 μg/ml (1/12500)) for 30 min at 4°C . The cells were simultaneously stained with CD3.

Acquisition of >30000 events were collected using a 50mW Yellow/Green laser (561nm) and 585/42 bandpass filter. Events were gated on viable cells.

Flow Cytometry (Intracellular) - PE Anti-CD3G antibody [EPR4517] (AB305817)
  • Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - PE Anti-CD3G antibody [EPR4517] (AB305817)

Flow cytometry staining of human peripheral blood mononuclear cells (PBMCs) with ab305817 (right) or Rabbit IgG (monoclonal) Phycoerythrin Isotype Control (ab209478) (left). Cells were fixed and permeabilised with BD Cytofix/Cytoperm™ for 20 min. PBMCs were incubated for 30 min at 4°C in 1x PBS containing 10 μg/ml human IgG and 10% normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody ab305817 or Rabbit IgG (monoclonal) Phycoerythrin Isotype Control (ab209478) (1x 106 in 100 μl at 0.04 μg/ml (1/12500)) for 30 min at 4°C . The cells were simultaneously stained with CD14.

Acquisition of >30000 events were collected using a 50mW Yellow/Green laser (561nm) and 585/42 bandpass filter. Events were gated on viable cells.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR4517

Isotype

IgG

Conjugation

PE

Excitation/Emission

Ex: 480;565nm, Em: 578nm

Carrier free

No

Reacts with

Human, Human, Mouse

Applications

Flow Cyt (Intra), ICC/IF, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/12500", "FlowCytIntra-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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Product details

This conjugated primary antibody is "made to order" and it is released using a quantitative quality control method that ensures binding affinity and labelling efficiency of the conjugate. Via leveraging the power of the Lightning-Link® conjugation technology, Abcam will deliver highly consistent recombinant conjugates in <2 weeks, giving you access to an ever growing portfolio of antibody-label combinations.

For suitable applications and species reactivity, please refer to the unconjugated version of this clone.

How are conjugated primary antibodies validated?
This conjugated primary antibody is released using a quantitative quality control method that evaluates binding affinity post-conjugation and efficiency of antibody labeling.
For suitable applications and species reactivity, please refer to the unconjugated version of this clone.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD247/CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways (PubMed : 2470098). In addition to this role of signal transduction in T-cell activation, CD3G plays an essential role in the dynamic regulation of TCR expression at the cell surface (PubMed : 8187769). Indeed, constitutive TCR cycling is dependent on the di-leucine-based (diL) receptor-sorting motif present in CD3G.
See full target information CD3G
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com