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Rabbit Recombinant Monoclonal CD3G antibody - conjugated to PE. Suitable for Flow Cyt (Intra) and reacts with Human samples.

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Images

Flow Cytometry (Intracellular) - PE Anti-CD3G antibody [EPR4517] (AB305817), expandable thumbnail
  • Flow Cytometry (Intracellular) - PE Anti-CD3G antibody [EPR4517] (AB305817), expandable thumbnail
  • Flow Cytometry (Intracellular) - PE Anti-CD3G antibody [EPR4517] (AB305817), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Conjugation

PE

Excitation/Emission

Ex: 480;565nm, Em: 578nm

Storage buffer

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98% PBS, 1% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow Cyt (Intra)
Human
Tested

Tested
Tested

Species

Human

Dilution info

1/12500

Notes

-

Associated Products

Select an associated product type

2 products for Alternative Version

Target data

Function

Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways (PubMed:2470098). In addition to this role of signal transduction in T-cell activation, CD3G plays an essential role in the dynamic regulation of TCR expression at the cell surface (PubMed:8187769). Indeed, constitutive TCR cycling is dependent on the di-leucine-based (diL) receptor-sorting motif present in CD3G.

Alternative names

T3G, T3G, CD3G, T-cell surface glycoprotein CD3 gamma chain, T-cell receptor T3 gamma chain

Recommended products

Rabbit Recombinant Monoclonal CD3G antibody - conjugated to PE. Suitable for Flow Cyt (Intra) and reacts with Human samples.

Key facts

Isotype

IgG

Conjugation

PE

Excitation/Emission

Ex: 480;565nm, Em: 578nm

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR4517

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle, Store in the dark

Notes

This conjugated primary antibody is “made to order” and it is released using a quantitative quality control method that ensures binding affinity and labelling efficiency of the conjugate. Via leveraging the power of the Lightning-Link® conjugation technology, Abcam will deliver highly consistent recombinant conjugates in <2 weeks, giving you access to an ever growing portfolio of antibody-label combinations.

For suitable applications and species reactivity, please refer to the unconjugated version of this clone.

This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

This conjugated primary antibody is released using a quantitative quality control method that evaluates binding affinity post-conjugation and efficiency of antibody labeling.
For suitable applications and species reactivity, please refer to the unconjugated version of this clone. This conjugated antibody is eligible for the Abcam trial program.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Flow Cytometry (Intracellular) - PE Anti-CD3G antibody [EPR4517] (ab305817), expandable thumbnail

    Flow Cytometry (Intracellular) - PE Anti-CD3G antibody [EPR4517] (ab305817)

    Flow cytometry staining of human peripheral blood mononuclear cells (PBMCs) with ab305817 (right) or Rabbit IgG (monoclonal) Phycoerythrin Isotype Control (PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab209478) (left). Cells were fixed and permeabilised with BD Cytofix/Cytoperm™ for 20 min. PBMCs were incubated for 30 min at 4°C in 1x PBS containing 10 μg/ml human IgG and 10% normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody ab305817 or Rabbit IgG (monoclonal) Phycoerythrin Isotype Control (PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab209478) (1x 106 in 100 μl at 0.04 μg/ml (1/12500)) for 30 min at 4°C . The cells were simultaneously stained with CD14.

    Acquisition of >30000 events were collected using a 50mW Yellow/Green laser (561nm) and 585/42 bandpass filter. Events were gated on viable cells.

  • Flow Cytometry (Intracellular) - PE Anti-CD3G antibody [EPR4517] (ab305817), expandable thumbnail

    Flow Cytometry (Intracellular) - PE Anti-CD3G antibody [EPR4517] (ab305817)

    Flow cytometry staining of human peripheral blood mononuclear cells (PBMCs) with ab305817 (right) or Rabbit IgG (monoclonal) Phycoerythrin Isotype Control (PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab209478) (left). Cells were fixed and permeabilised with BD Cytofix/Cytoperm™ for 20 min. PBMCs were incubated for 30 min at 4°C in 1x PBS containing 10 μg/ml human IgG and 10% normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody ab305817 or Rabbit IgG (monoclonal) Phycoerythrin Isotype Control (PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab209478) (1x 106 in 100 μl at 0.04 μg/ml (1/12500)) for 30 min at 4°C . The cells were simultaneously stained with CD19.

    Acquisition of >30000 events were collected using a 50mW Yellow/Green laser (561nm) and 585/42 bandpass filter. Events were gated on viable cells.

  • Flow Cytometry (Intracellular) - PE Anti-CD3G antibody [EPR4517] (ab305817), expandable thumbnail

    Flow Cytometry (Intracellular) - PE Anti-CD3G antibody [EPR4517] (ab305817)

    Flow cytometry staining of human peripheral blood mononuclear cells (PBMCs) with ab305817 (right) or Rabbit IgG (monoclonal) Phycoerythrin Isotype Control (PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab209478) (left). Cells were fixed and permeabilised with BD Cytofix/Cytoperm™ for 20 min. PBMCs were incubated for 30 min at 4°C in 1x PBS containing 10 μg/ml human IgG and 10% normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody ab305817 or Rabbit IgG (monoclonal) Phycoerythrin Isotype Control (PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab209478) (1x 106 in 100 μl at 0.04 μg/ml (1/12500)) for 30 min at 4°C . The cells were simultaneously stained with CD3.

    Acquisition of >30000 events were collected using a 50mW Yellow/Green laser (561nm) and 585/42 bandpass filter. Events were gated on viable cells.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com